Artificial hybridization between Robinia pseudoacacia L. and R. pseudoacacia var. monophylla Carr.

Dini-Papanastasi, O.; Aravanopoulos, Filippos A.

doi:10.1093/forestry/cpm047

Cited by 10 publications

(10 citation statements)

References 13 publications

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“…Важливим компонентом міських екосистем є Robinia pseudoacacia L. (відома як «чорна сарана» або «несправжня акація»), яка, з одного боку, є інвазивним видом (активно витісняє місцеву флору в природному середовищі існування завдяки швидкому проростанню і росту тощо), а з іншого -корисною багатоцільовою деревною породою, яку використовують на пиломатеріали, стовпи, паливо, в меліорації, тваринництві, бджільництві, для отримання деревних волокон та кормів (Bongarten et al, 1992;Dini-Papanastasi and Aravanopoulos, 2008;Wojda et al, 2015). Вважається, що R. pseudoacacia швидко натуралізувалася завдяки невеликій кількості природних ворогів у нових умовах існування (Європа), а також через високу адаптивність цього виду (Enescu and Dӑnescu, 2013;Vítková et al, 2017;Wilkaniec et al, 2021).…”

Section: вступunclassified

Peroxidase activity and isoenzyme composition in Robinia pseudoacacia L. leaves under the influence of Parectopa robiniella Clemens, 1863

Шупранова¹,

Holoborodko²,

Huslystyi³

et al. 2021

Agro

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Іn recent times R. pseudoacacia has been suffering from populations of new pests, which cause serious economic damage to this strategic species. Therefore, studying the features of plant defense responses against harmful insects is of great importance for biodiversity conservation for preserving this species in natural and artificial plantings. The aim of the study was to analyze the peculiarities of the activity and isoenzyme composition of peroxidase in R. pseudoacacia leaves during attacks by the invading insect P. robiniella. In the green plantations of Dnipro city, as the object of research, six groups of Robinia pseudoacacia L. model trees of different ages (5 and 35–40 years of age) with the existing leaf damages by P. robiniella leaf blotch miner were identified. Differences in benzidine-peroxidase activity were found in the leaves of 5- and 40-year-old R. pseudoacacia trees. In 35‒40-year-old trees with the presence of leaf blotch miners, the leaves showed a tendency to increase BPOD activity by 24.6% vs control. While in young plants, a significant increase in peroxidase activity was found by 3.8 times due to mechanical damage of the leaves by the miner compared to the control. The revealed significant increase (by 63.2%) in the activity of guaiacol-peroxidase of black locust indicates its active participation in enhancing the barrier functions of leaf cells against P. robiniella, and it indicates the promising use of the enzyme as a biomarker for pest influence. The composition of benzidine-peroxidase isoenzymes was analyzed by electrofocusing. The main pattern of changes in the expressiveness of benzidine-peroxidase due to damage of leaves by a pest was a quantitative redistribution of activity between different molecular forms of peroxidase. Several enzyme isoforms from the highly acidic pH zone showed a significant activity increase in R. pseudoacacia leaves damaged by P. robiniella; this especially applies to the peroxidase system of young black locust plants. The increase in bensidine- and guajacol-peroxidase activity under the influence of leaf miner may be explained by a change in the gene expression of individual isoforms of peroxidases, which is confirmed by the results of studying the relative share of peroxidase molecular forms with isoelectrofocusing. Calculation of the internal diversity index (KD) of IEF benzidine-peroxidase spectra showed that the studied peroxidase spectra of young and medieval trees of control and experimental samples of R. pseudoacacia leaves differ significantly in the level of complexity. Under the influence of the invader the level of complexity increased. The study of the reaction of black locust and the mechanisms of its protection against mechanical damage by phytophages with the involvement of a larger range of enzymes in further research can serve as a theoretical basis for species selection and management to restore it under biotic stress.

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Section: вступunclassified

Peroxidase activity and isoenzyme composition in Robinia pseudoacacia L. leaves under the influence of Parectopa robiniella Clemens, 1863

Шупранова¹,

Holoborodko²,

Huslystyi³

et al. 2021

Agro

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“…Dini-Papanastasi et al [39] and Surles et al [11] reported that black locust is a highly outcrossing tree species, and no seeds can be obtained by selfing. However, Sun [10] and Yuan [12] showed that black locust has a mating system based on outcrossing, but selfing can also produce offspring.…”

Section: Mating System Of Black Locustmentioning

confidence: 99%

“…Due to its low seed-setting rate and the damage caused by castration, hybridization of black locust is problematic [9][10][11][12]39]. Therefore, we conducted controlled pollination without castration.…”

Section: Controlled Pollination Of Black Locust Without Castrationmentioning

confidence: 99%

Pollen Competition and Paternal Contribution during Artificially Controlled Pollination of Black Locust (Robinia pseudoacacia L.) without Castration

Sun

Dong

et al. 2021

Forests

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(1) Background: Considering the serious damage caused by castration and the extremely high outcrossing rate in nature, we hypothesized that artificial controlled pollination of black locust without castration could be conducted for hybridization breeding. (2) Methods: The study conducted controlled pollination on 20 mating combinations of black locust without castration using a single or mixed male parent. Offspring of different developmental stages and the leaves of parents were collected to extract DNA and perform paternity analysis using SSR molecular markers. (3) Results: The contribution rate of each male parent differed according to developmental stage after pollination using different pollens mixed in equal proportions. There were significant correlations between the genetic similarity between each male parent and female parent and contribution rate of each male parent at three different developmental stages after pollination. (4) Conclusions: The composition of offspring pollen donors showed no bias toward selfing or outcrossing when artificially pollinated without castration. Hybrid breeding of black locust by artificially controlled pollination without castration may not be feasible, given that our manual method resulted in a large number of abortive and abnormal offspring. Introduction of honeybees in a limited space to conduct controlled pollination of black locust for hybrid breeding may be feasible.

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“…Robinia pseudoacacia L. has become an important pioneer afforestation tree in northwest China because of it can withstand slight saline-alkaline and dry soils and has grown well in barren mountains [ 25 ]. In addition, the black locust is an economically valuable tree: bees feed on its flowers to produce honey, the forest rapidly growths, and the durability and strength its timbers make it suitable for building [ 26 – 28 ]. However, the haphazard introduction of black locust into many areas of China and a related lack of records about introduced samples have led to confusion concerning the black locust germplasm resources in China.…”

Section: Introductionmentioning

confidence: 99%

Variation of phenotypic and physiological traits of Robinia pseudoacacia L. from 20 provenances

et al. 2022

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To select elite Robinia pseudoacacia L. germplasm resources for production, 13 phenotypes and three physiological indicators of 214 seedlings from 20 provenances were systematically evaluated and analyzed. The leaf phenotypic and physiological coefficients of variation among the genotypes ranged from 3.741% to 19.599% and from 8.260% to 42.363%, respectively. The Kentucky provenance had the largest coefficient of variation (18.541%). The average differentiation coefficients between and within provenances were 34.161% and 38.756%, respectively. These close percentages showed that R. pseudoacacia presented high genetic variation among and within provenances, which can be useful for assisted migration and breeding programs. Furthermore, based on the results of correlations, principal component analysis and cluster analysis, breeding improvements targeting R. pseudoacacia’s ornamental value, food value, and stress resistance of were performed. Forty and 30 excellent individuals, accounting for 18.692% and 14.019%, respectively, of the total resources. They were ultimately screened, after comprehensively taking into considering leaf phenotypic traits including compound leaf length, leaflet number and leaflet area and physiological characteristics including proline and soluble protein contents. These selected individuals could provide a base material for improved variety conservation and selection.

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Artificial hybridization between Robinia pseudoacacia L. and R. pseudoacacia var. monophylla Carr.

Cited by 10 publications

References 13 publications

Peroxidase activity and isoenzyme composition in Robinia pseudoacacia L. leaves under the influence of Parectopa robiniella Clemens, 1863

Peroxidase activity and isoenzyme composition in Robinia pseudoacacia L. leaves under the influence of Parectopa robiniella Clemens, 1863

Pollen Competition and Paternal Contribution during Artificially Controlled Pollination of Black Locust (Robinia pseudoacacia L.) without Castration

Variation of phenotypic and physiological traits of Robinia pseudoacacia L. from 20 provenances

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