Artificial controlled model of blood circulation system for adhesive evaluation

Abstract

Since there are several casualties due to uncontrolled bleeding resulting from simple injury to surgery, effective styptic or vessel adhesives are important; however, their development is limited by the lack of standardized systems to evaluate potential compounds. The current study outlines the development of an aorta styptic evaluation system, comprising of decellularized swine aorta tissue and a heart pump-mimicking system. Although the cells in the swine aorta were removed, the structural stability of the aorta was sustained due to the maintenance of the extracellular matrix. Using a control adhesive, Cyanoacrylate, the developed model was found to have similar adhesive efficacy to intact aorta. The circulatory-mimicking system was designed to mimic the beat rate and strength of blood-flow from the heart, which was necessary to evaluate the adherent efficacy. The decellularized aorta improves instabilities of intact tissues, which occurs on account of storage and origin, thereby allowing for a more standardized system. The system was able to simulate several symptoms of circulation, according to patient age and health, by adjusting pumping frequency and intensity. Therefore, this system can be used as a standardized evaluation system for screening adhesives. Further, it would also evaluate other medical devices, such as stent or medications.

Recent advances in soluble decellularized extracellular matrix for heart tissue engineering and organ modeling

“…These results prove the efficacy of the decellularized matrix to serve as a perfect model system. 28 Hogan et al have wrapped a fibrin patch containing active cardiomyocyte cells around decellularized adult rat hearts to fabricate a functional bioartificial heart muscle model. 29 Their results show that after 1-2 days of culture, the construct was beating normally with a contractile rate of 4.5 Hz.…”

Reconstruction of the Physiological Behavior of Real and Synthetic Vessels in Controlled Conditions

“…Human organs are complex structures composed of specialized cells that collaboratively execute unique functions that are crucial for survival [1]. Ethical considerations necessitate the utilization of bio-tissue and mechanical systems that can replicate the physiological and pathophysiological aspects of organs [2]. Such systems hold the potential to provide enhanced predictive capabilities for human tissue responses compared to existing testing methodologies.…”