Artificial Control of Cell Signaling and Growth by Magnetic Nanoparticles

Lee, Jae Hyun; Kim, Eun Sook; Cho, Mi Hyeon; Son, Mina; Yeon, Soo In; Shin, Jeon Soo; Cheon, Jinwoo

doi:10.1002/anie.201001149

Cited by 71 publications

(25 citation statements)

References 24 publications

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“…Endothelial cells in the regeneration compartment play a pivotal role in tissue regeneration and organogenesis by secreting autocrine and paracrine factors that are critical for healing, and contribute to angiogenesis by becoming the main components of the new blood vessels [63,64]. Angiogenesis can be stimulated by manipulating integrins through magnetic twisting ( Figure 1B) or the endothelial receptor cell TIE Tyrosine Kinase 2 (TIE2) through magnetic clustering ( Figure 1C) [65,66]. Manipulation through magnetic twisting is achieved using magnetic beads functionalised with RGD-peptide sequences to bind endothelial integrins.…”

Section: Magnetic Actuation In the Regeneration Compartmentmentioning

confidence: 99%

See 1 more Smart Citation

Harnessing magnetic-mechano actuation in regenerative medicine and tissue engineering

Santos¹,

Reis²,

Gomes³

2015

Trends in Biotechnology

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Section: Magnetic Actuation In the Regeneration Compartmentmentioning

confidence: 99%

“…Manipulation through clustering is done using magnetic beads functionalised with TIE2 antibodies to target the receptor [65]. Application of a magnetic field clusters the targeted receptors and activates them, resulting in an increase in the tube-length of vessel-like structures [65].…”

Section: Magnetic Actuation In the Regeneration Compartmentmentioning

confidence: 99%

Harnessing magnetic-mechano actuation in regenerative medicine and tissue engineering

Santos¹,

Reis²,

Gomes³

2015

Trends in Biotechnology

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“…[1][2][3][4][5][6][7][8][9][10] Because of its attractive potential to enable highly sensitive MRI application, many research efforts have been dedicated on developing nanoparticulate MNPs-based contrast agent. These included magnetically guided and targeted drug delivery of drug and gene payloads under remote fields, carrier for magnetic bio-separation agent, heat generation agent for magnetic hyperthermia cancer therapy, advanced cellular control over the cellular activities as well as providing contrast enhancement effect in magnetic resonance imaging (MRI).…”

Section: Introductionmentioning

confidence: 99%

Engineered water-soluble two-dimensional magnetic nanocomposites: towards highly magnetic relaxometric properties

et al. 2015

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Water dispersible two-dimensional magnetic nanocomposites are formed by phase-transferring hydrophobic manganese-doped ferrite nanoparticles (MFPs) into aqueous solvent using a one-step simple approach involving only graphene oxide (GO) as the phase transfer agent. The resultant hydrophilic magnetic nanocomposites (MFNs) are surprisingly stable in the aqueous phase despite its large hydrodynamic size (dhyd). Because of its unique construct that promotes water accessibility towards the MFP core, large MFNs loaded with an 18 nm MFP core (MFN-18; dhyd = 577.9 nm) exhibits transverse relaxivity (r2) up to ∼6.8 times (r2 = 800.8 mM [Mn + Fe](-1) s(-1)) higher than the typical individually coated MFP-18 with amphiphilic brush copolymers (r2 = 117.3 mM [Mn + Fe](-1) s(-1)). Meanwhile, the overall nanocomposites dhyd can be further reduced by employing a smaller pre-sonicated GO sheet phase transfer agent. As a result of using small GO sheets with enhanced hydrophilicity, the r2 of small MFN-18* nanocomposites (dhyd = 224.9 nm) increases by approximately 37% (r2 = 1097.4 mM [Mn + Fe](-1) s(-1)) as compared to larger MFN-18. From a simple comparative study among various magnetic nanocomposites involving a MFP-18 core, the high MFN-18 r2 relaxivity value can be attributed to enhanced water diffusion and exchange due to the GO sheet, allowing better interaction between magnetic the MFP core and water protons. The proposed method can be readily extended to convert other types of hydrophobic nanoparticles into water-dispersible nanocomposites.

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“…In this context, magnetic actuation of nanoparticles provides a novel strategy for controlling cells that is complementary to genetic, chemical and optogenetic tools 14 . For instance, recent key experiments have demonstrated the magnetic actuation of signalling pathways using nanoparticles conjugated to antibodies by inducing the clustering of extracellular receptors 15,16 or through endocytic internalization 17 . In these studies, nanoparticles are no longer passive reporters of biomolecules, but on interaction with a magnetic field actuate a biological response.…”

mentioning

confidence: 99%

Spatiotemporal control of microtubule nucleation and assembly using magnetic nanoparticles

et al. 2013

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Decisions on the fate of cells and their functions are dictated by the spatiotemporal dynamics of molecular signalling networks. However, techniques to examine the dynamics of these intracellular processes remain limited. Here, we show that magnetic nanoparticles conjugated with key regulatory proteins can artificially control, in time and space, the Ran/RCC1 signalling pathway that regulates the cell cytoskeleton. In the presence of a magnetic field, RanGTP proteins conjugated to superparamagnetic nanoparticles can induce microtubule fibres to assemble into asymmetric arrays of polarized fibres in Xenopus laevis egg extracts. The orientation of the fibres is dictated by the direction of the magnetic force. When we locally concentrated nanoparticles conjugated with the upstream guanine nucleotide exchange factor RCC1, the assembly of microtubule fibres could be induced over a greater range of distances than RanGTP particles. The method shows how bioactive nanoparticles can be used to engineer signalling networks and spatial self-organization inside a cell environment.

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Artificial Control of Cell Signaling and Growth by Magnetic Nanoparticles

Cited by 71 publications

References 24 publications

Harnessing magnetic-mechano actuation in regenerative medicine and tissue engineering

Harnessing magnetic-mechano actuation in regenerative medicine and tissue engineering

Engineered water-soluble two-dimensional magnetic nanocomposites: towards highly magnetic relaxometric properties

Spatiotemporal control of microtubule nucleation and assembly using magnetic nanoparticles

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