Artificial chromosome formation in maize (Zea mays L.)

Ananiev, E. V.; Wu, Chun; Chamberlin, Mark A.; Svitashev, Sergei; Schwartz, Chris; Gordon‐Kamm, William; Tingey, Scott V.

doi:10.1007/s00412-008-0191-3

Cited by 78 publications

(62 citation statements)

References 95 publications

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“…Treatments included (1) moGFP alone, (2) moGFP plus Wus2, (3) moGFP plus Bbm, or (4) moGFP plus both morphogenic genes. Gold particle preparation, attachment of the DNA mixture to the 0.6-mm gold particles, and bombardment parameters were described by Ananiev et al (2009), with the following changes in the attachment protocol. The DNA was coated onto 0.6-mm (average diameter) gold particles using a water-soluble cationic lipid Tfx-50 (Promega) as follows: A DNA mixture was prepared by adding separate plasmids, containing 50 ng GFP plasmid plus 100 ng GUS plasmid (Trt.…”

Section: Particle Gun Transient Phenotype Observationsmentioning

confidence: 99%

Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation

et al. 2016

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While transformation of the major monocot crops is currently possible, the process typically remains confined to one or two genotypes per species, often with poor agronomics, and efficiencies that place these methods beyond the reach of most academic laboratories. Here, we report a transformation approach involving overexpression of the maize (Zea mays) Baby boom (Bbm) and maize Wuschel2 (Wus2) genes, which produced high transformation frequencies in numerous previously nontransformable maize inbred lines. For example, the Pioneer inbred PHH5G is recalcitrant to biolistic and Agrobacterium tumefaciens transformation. However, when Bbm and Wus2 were expressed, transgenic calli were recovered from over 40% of the starting explants, with most producing healthy, fertile plants. Another limitation for many monocots is the intensive labor and greenhouse space required to supply immature embryos for transformation. This problem could be alleviated using alternative target tissues that could be supplied consistently with automated preparation. As a major step toward this objective, we transformed Bbm and Wus2 directly into either embryo slices from mature seed or leaf segments from seedlings in a variety of Pioneer inbred lines, routinely recovering healthy, fertile T0 plants. Finally, we demonstrated that the maize Bbm and Wus2 genes stimulate transformation in sorghum (Sorghum bicolor) immature embryos, sugarcane (Saccharum officinarum) callus, and indica rice (Oryza sativa ssp indica) callus.

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Section: Particle Gun Transient Phenotype Observationsmentioning

confidence: 99%

Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation

et al. 2016

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“…The CentC/CRM arrays span several megabases of DNA in some maize centromeres (Jin et al 2004;Ananiev et al 2009). The CENH3-binding domains of these heavily repetitive centromeres generally cannot be delineated by sequencing-based approaches.…”

Section: Mapping the Cenh3-binding Domain Of Neom3 (Ncenm3 )mentioning

confidence: 99%

Maize centromeres expand and adopt a uniform size in the genetic background of oat

Wang

Zhang

et al. 2013

Genome Res.

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Most existing centromeres may have originated as neocentromeres that activated de novo from noncentromeric regions. However, the evolutionary path from a neocentromere to a mature centromere has been elusive. Here we analyzed the centromeres of nine chromosomes that were transferred from maize into oat as the result of an inter-species cross. Centromere size and location were assayed by chromatin immunoprecipitation for the histone variant CENH3, which is a defining feature of functional centromeres. Two isolates of maize chromosome 3 proved to contain neocentromeres in the sense that they had moved from the original site, whereas the remaining seven centromeres (1, 2, 5, 6, 8, 9, and 10) were retained in the same area in both species. In all cases, the CENH3-binding domains were dramatically expanded to encompass a larger area in the oat background (∼3.6 Mb) than the average centromere size in maize (∼1.8 Mb). The expansion of maize centromeres appeared to be restricted by the transcription of genes located in regions flanking the original centromeres. These results provide evidence that (1) centromere size is regulated; (2) centromere sizes tend to be uniform within a species regardless of chromosome size or origin of the centromere; and (3) neocentromeres emerge and expand preferentially in gene-poor regions. Our results suggest that centromere size expansion may be a key factor in the survival of neocentric chromosomes in natural populations.

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“…However, there is no such system for plants. Recently three reports have been published on the production of plant artificial chromosomes (Carlson et al, 2007;Yu et al, 2007;Ananiev et al, 2009). However, there is still no report of utilizing them for plant transformation with large DNA fragments.…”

Section: Fig 3 Schematic Diagram Of a Bead-makermentioning

confidence: 99%