Artificial carbon assimilation: From unnatural reactions and pathways to synthetic autotrophic systems

Yang, Xue; Zhang, Yanfei; Zhao, Guoping

doi:10.1016/j.biotechadv.2023.108294

Cited by 3 publications

(1 citation statement)

References 193 publications

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“…[ 18,19 ] These advances have made the biological assimilation of methanol and formate increasingly equivalent to the direct assimilation of CO 2 in terms of technological feasibility. [ 20 ] Formate, in addition to its efficient production through CO 2 hydrogenation reduction, is distinguished by its low toxicity and the ability to exist as a liquid or solid at room temperature, rendering it an easily accessible fermentation feedstock. Currently, the primary natural assimilation pathways for formate include the serine cycle and reductive glycine pathway.…”

Section: Introductionmentioning

confidence: 99%

Unlocking the formate utilization of wild‐type Yarrowia lipolytica through adaptive laboratory evolution

Chen,

Hou

et al. 2024

Biotechnology Journal

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Synthetic biology is contributing to the advancement of the global net‐negative carbon economy, with emphasis on formate as a member of the one‐carbon substrate garnering substantial attention. In this study, we employed base editing tools to facilitate adaptive evolution, achieving a formate tolerance of Yarrowia lipolytica to 1 M within 2 months. This effort resulted in two mutant strains, designated as M25‐70 and M25‐14, both exhibiting significantly enhanced formate utilization capabilities. Transcriptomic analysis revealed the upregulation of nine endogenous genes encoding formate dehydrogenases when cultivated utilizing formate as the sole carbon source. Furthermore, we uncovered the pivotal role of the glyoxylate and threonine‐based serine pathway in enhancing glycine supply to promote formate assimilation. The full potential of Y. lipolytica to tolerate and utilize formate establishing the foundation for pyruvate carboxylase‐based carbon sequestration pathways. Importantly, this study highlights the existence of a natural formate metabolic pathway in Y. lipolytica.

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Section: Introductionmentioning

confidence: 99%

Unlocking the formate utilization of wild‐type Yarrowia lipolytica through adaptive laboratory evolution

Chen,

Hou

et al. 2024

Biotechnology Journal

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Recent Progress in the Synthesis of α‐Hydroxy Carbonyl Compounds with ThDP‐dependent Carboligases

Dobiašová,

Jurkaš,

Both

et al. 2024

ChemCatChem

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Carboligases catalyze the thiamine diphosphate (ThDP) dependent formation of carbon‐carbon bonds. These enzymes are prominent biocatalysts for the production of valuable a‐hydroxy carbonyl compounds, which serve as key building blocks in the synthesis of various pharmaceuticals and fine chemicals. Carboligases act in selective manner to afford regio‐ and stereochemically defined products from simple starting materials. This review explores the catalytic prowess of carboligases for synthetic purposes and is aimed at providing a selection tool of enzymes for particular sets of substrates or desired products. The comprehensive overview encompasses structural insights, relationships of the currently known sequence space and practical applications with a focus on recent literature, showcasing carboligases as potent tools for sustainable and efficient synthesis.

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Uncovering the baccatin III biosynthetic pathway for sustainable taxoid supply

Tan,

Chen

2024

Science Bulletin

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Artificial carbon assimilation: From unnatural reactions and pathways to synthetic autotrophic systems

Cited by 3 publications

References 193 publications

Unlocking the formate utilization of wild‐type Yarrowia lipolytica through adaptive laboratory evolution

Unlocking the formate utilization of wild‐type Yarrowia lipolytica through adaptive laboratory evolution

Recent Progress in the Synthesis of α‐Hydroxy Carbonyl Compounds with ThDP‐dependent Carboligases

Uncovering the baccatin III biosynthetic pathway for sustainable taxoid supply

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