Artifactual changes in hematological variables in equine blood samples stored at different temperatures and various anticoagulants

Sharif, Meysam Tehrani; Mahabadi, Mehrdad Ameri; Moshfeghi, S.; Sharifi, Hamid; Hoseini, Seyed Javad; Alavi, Seyed Mohsen

doi:10.1007/s00580-010-1116-0

Cited by 6 publications

(4 citation statements)

References 13 publications

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“…On the contrary, when using sodium citrate, most hematological parameters significantly decrease, compared with the other anticoagulants (Sharif et al, 2010).…”

Section: Sampling Handlingmentioning

confidence: 79%

“…The most common change associated with storage is an increase in the RBC size, a fact that artefactually leads to increased MCV and PCV (Allen et al, 1988). However, within limitations in some hematological parameters, equine blood samples stored in EDTA at 4ºC for a maximum of 72 hrs may be adequate for blood tests (Sharif et al, 2010).…”

Section: Sampling Handlingmentioning

confidence: 99%

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Physiological Factors in the Interpretation of Equine Hematological Profile

Satué¹,

Hernández²,

Muñoz³

2012

Hematology - Science and Practice

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“…On the contrary, when using sodium citrate, most hematological parameters significantly decrease, compared with the other anticoagulants (Sharif et al, 2010).…”

Section: Sampling Handlingmentioning

confidence: 79%

Section: Sampling Handlingmentioning

confidence: 99%

Physiological Factors in the Interpretation of Equine Hematological Profile

Satué¹,

Hernández²,

Muñoz³

2012

Hematology - Science and Practice

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“…The samples were always taken on the same day of the week and analysed the next day, after being kept at 4 °C overnight. Therefore, all samples were treated in the same way and so were suitable for haematological analysis and comparable between sampling dates [ 58 ]. This method of sample collection and handling reduced the reliability of platelet values [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Repeated Blood Harvesting from Pregnant Mares on Haematological Variables

Oddsdóttir,

Jónsdóttir,

Sturludóttir

et al. 2024

Animals

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Studies have been carried out on the effect of large-volume blood harvesting from horses, but they were performed on nonpregnant horses of various breeds other than Icelandic horses. This study aimed to investigate the effect on the haematological variables indicating erythropoiesis of repeated blood harvesting from pregnant mares. To account for regional variation, two herds of mares were chosen, both kept under free-range conditions. Sequential EDTA blood samples were collected weekly from 160 mares and analysed for haematological variables in an automated analyser. Serum samples from 115 mares were analysed for total protein. In both herds, after three harvests, mares began to measure below the minimum value for erythrocyte numbers, and mild anaemia was present in up to 37% at one time. Mares in only one herd had moderate or marked anaemia, 14.3% of the herd. Both herds showed evidence of increased erythropoiesis, but there was a difference between the herds in the intensity of the response. In both herds, however, all mares had reached the minimum normal haematocrit value three weeks after the last harvest. It is important to investigate the causes for the differences between these herds, which might be addressed to reduce the risk of anaemia.

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“…Moreover, there are significant differences in total white blood count and total lymphocyte count between sodium citrate and EDTA. It has been reported that those parameters are decreased in blood samples drawn onto sodium citrate [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Influence of blood sample storage and different types of anticoagulants on results of NK cytotoxicity tests

Popko,

Paskudzka,

Pieśniewska

et al. 2023

cejoi

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Introduction Natural killer (NK) cells are important players in the human immune response. Impaired NK function may lead to serious, life-threatening conditions. Defects may be consequences of genetic mutations or results of secondary factors such as infections, malignancies and autoimmune diseases. The cytotoxicity test is very useful, but its accessibility is limited to special immunological laboratories. Blood samples are often transported to remote centers, which takes time and requires special conditions. The aim of this study was to compare cytotoxicity assay results between samples preserved with three different anticoagulants to standardize the diagnostic procedure. Material and methods Peripheral blood from healthy donors was taken with three anticoagulants: heparin, K 2 EDTA and citrate. Peripheral blood mononuclear cells (PBMC) were isolated and tested directly after blood drawing and after 24-hour storage. Cytotoxic abilities of NK cells were tested in 4 h co-culture with K562. NK cytotoxicity was measured by flow cytometry. Results In most cases of analyzed healthy donors, cytotoxicity results were similar regardless of type of anticoagulant. However, the highest mean values were obtained in samples with citrate. There was a significant decrease in cytotoxicity after 24 hours of storage of the whole blood at ambient temperature. The mean drop in cytotoxicity results was substantial for all anticoagulants: 76% for heparin, 67% for citrate and 70% for EDTA. Conclusions Results of spontaneous NK cytotoxicity seem to be affected by the anticoagulants used for blood protection. Commercial instant cytotoxicity testing and delayed analysis after blood storage gave the highest results in blood with sodium citrate.

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Artifactual changes in hematological variables in equine blood samples stored at different temperatures and various anticoagulants

Cited by 6 publications

References 13 publications

Physiological Factors in the Interpretation of Equine Hematological Profile

Physiological Factors in the Interpretation of Equine Hematological Profile

The Effect of Repeated Blood Harvesting from Pregnant Mares on Haematological Variables

Influence of blood sample storage and different types of anticoagulants on results of NK cytotoxicity tests

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