Artifacts in histology: A 1-year retrospective study

Igho, Odokuma Emmanuel; Aimakhume, Aifuobhokhan

doi:10.4103/2315-7992.210253

Cited by 5 publications

(9 citation statements)

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“…Most of the tissue sections had more than one pattern of artifacts which is similar to the present study. They observed artifacts in 94.58% of sections similar to the present study and fold artifact accounted for majority of the cases [5] . Though the use of DPX an mountant media has an advantage that it can be peeled off early, it can give a foggy appearance.…”

Section: Discussionsupporting

confidence: 86%

Artifacts: A menace in histopathology

Satapute¹,

Shashikala²,

Gu³

2020

Int. J. Clin. Diagn. Pathol.

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Introduction: The word 'artifact' is derived from the latin words, 'Ars' (art) & 'factum'(made). The study was undertaken to know the prevalence and patterns of artifacts. Materials & methods: This is a cross-sectional observational study. 250 consecutive hematoxylin and eosin (H&E) stained sections were retrieved from the archives and analyzed for artifacts under light microscope. Results: Among 250 slides, 227(90.8%) showed the presence of artifacts. The folding artifact was the most prevalent constituting 98(43.17%) and excess mountant 6(2.64%) was least common. Conclusion: Folding artifacts were the most prevalent pattern in this study. Proper technical measures need to be employed in order to prevent/minimize the occurrence of artifacts in a skillful manner, as they may pose diagnostic difficulties.

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Section: Discussionsupporting

confidence: 86%

Artifacts: A menace in histopathology

Satapute¹,

Shashikala²,

Gu³

2020

Int. J. Clin. Diagn. Pathol.

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“…In this study, the majority of histopathological H&E-stained sections had more than one type of microtomy artefacts. This finding differs from a study done by Igho et al in Nigeria which showed that the majority of H&E-stained slides had one kind of artefacts [10]. The probable reason for the low number of histopathological sections to have more than one type of artefacts compared to our study was due to low number of tissue slides assessed (388 histological tissue slides)…”

Section: Discussioncontrasting

confidence: 99%

“…Microtomy artefacts were introduced into the tissue during the techniques of tissue sectioning, trimming, and tissue flotation [6,9]. The commonest reason for microtomy artefacts to occur in histopathology laboratories were lack of regular training for technical staff, overuse of microtomy machines without maintenance, lack of supervision in the analytical phase of histopathology laboratory, and a low number of technical staff, and overwork [8,10]. Microtomy artefacts varied from the smaller ones to the largest ones, due to this some artefacts may be able to make the tissue architecture non-diagnostic and finally difficult to the interpretation of slides result and delay in turnaround time [4].…”

Section: Discussionmentioning

confidence: 99%

Histopathological Evaluation of the Microtomy Artifacts on Haematoxylin and Eosin Section; Hospital Based Cross-Sectional Study

Ottoman

Urassa

Elias

et al. 2022

East Afr. j. health sci.

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Background information: Microtomy artifacts are abnormal structures or features in histological slides resulting from tissue sectioning by microtome. Objective: To determine the type and prevalence of microtomy artifacts found in histopathological tissue sections slides at Bugando Medical Centre (BMC). Methodology: This was a cross-sectional observational study that involved 547 consecutive hematoxylins and eosin (H&E) stained sections of histological archived tissue slides of January 2021. The slides were retrieved from the archives of the histopathology laboratory at BMC, Mwanza Tanzania and analysed for artifacts under a light microscope. Results: A total number of 547 histopathological slides were retrieved for the study and 412 (75.3%) slides had microtomy artifacts present while the remaining 135 (24.7%) histopathological slides had no microtomy artifacts. Of 412 slides with microtomy artifacts, 204(49.5%) slides had only one type of microtomy artifacts while the remaining 208 (50.5%) slides had more than one type of microtomy artifacts. There was a total of 672 microtomy artifacts, and the majority 576 (85.7%) were due to section cutting, followed by trimming artifacts in 92 (13.69%) of the slides. The least artifact was floatation which was seen in 4 (0.6%) of the slides. For the floatation artifact, the folding artifact was the most commonly seen in 300(54.8%) of the slides. Conclusion: Higher prevalence of microtomy artifacts at BMC reflects the problem of interpretation of histopathological slides in our setting. Section folding artifacts were the most prevalent pattern of artifact observed in this study.

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“…The most widely used stain for both diagnostic and research histology is H&E. Artifacts in stained slides may result from improper tissue fixation, poor dehydration, poor microtome sectioning, and variable stain composition. 39,40 Artifacts may cause different anatomical structures to look abnormally similar, or for some structures to disappear or become highly salient from one section to another. This complicates spatial alignment between histology sections, especially when a validation process extends in three dimensions.…”

Section: Tissue Stainingmentioning

confidence: 99%

“…Unstained tissue sections are nearly invisible with light microscopy, so staining is used to create contrast. The most widely used stain for both diagnostic and research histology is H&E. Artifacts in stained slides may result from improper tissue fixation, poor dehydration, poor microtome sectioning, and variable stain composition 39,40 . Artifacts may cause different anatomical structures to look abnormally similar, or for some structures to disappear or become highly salient from one section to another.…”

Section: Ref Tissue Similarity Based On: Transform Space Modification...mentioning

confidence: 99%

Histological Validation of MRI: A Review of Challenges in Registration of Imaging and Whole‐Mount Histopathology

Alyami

Kyme

Bourne

2020

Magnetic Resonance Imaging

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Rigorous validation with ground truth information such as histology is needed to reliably assess the current and potential value of MRI techniques to characterize tissue and identify disease‐related tissue alterations. Commonly used methods that aim to directly correlate histology and MRI data generally fall short of this goal due to spatial errors that preclude direct matching. Errors result from tissue deformation, differences in spatial resolution and slice thickness, non‐coplanar and/or nonintersecting plane orientations, and different image contrast mechanisms. Some of these problems arise from limitations in standard protocols for clinical tissue processing and histology‐based pathology reporting, and to some extent can be addressed by modifications to standard protocols without compromising the clinical process. Typical modifications include ex vivo specimen MRI, block‐face photography, addition of fiducial markers, and 3D printed molds to constrain tissue deformation and guide sectioning. This review summarizes the advantages and limitations of MRI validation techniques based on coregistration of MRI with whole‐mount histology of tissue specimens. Level of Evidence 4 Technical Efficacy Stage 1

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Artifacts in histology: A 1-year retrospective study

Cited by 5 publications

References 1 publication

Artifacts: A menace in histopathology

Artifacts: A menace in histopathology

Histopathological Evaluation of the Microtomy Artifacts on Haematoxylin and Eosin Section; Hospital Based Cross-Sectional Study

Histological Validation of MRI: A Review of Challenges in Registration of Imaging and Whole‐Mount Histopathology

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