Artifact-reduced simultaneous MRI of multiple rats with liver cancer using PROPELLER

Abstract

PROPELLER allowed simultaneous acquisition of artifact-reduced T2 - and diffusion-weighted images of multiple hepatoma-bearing rats. This technique can promote high-throughput preclinical MR research for liver cancer.

Establishing Magnetic Resonance Imaging as an Accurate and Reliable Tool to Diagnose and Monitor Esophageal Cancer in a Rat Model

“…[24], [25]. Since MRI had recently been utilized successfully in small animal translational models for other types of cancers [20]–[22], we aimed to test the ability of MRI to effectively diagnose esophageal tumor in the Levrat model as compared to endoscopy. As noted, we found MRI to be a very valid and reliable method for detection of esophageal tumors as compared to endoscopy.…”

“…Recently, magnetic resonance imaging (MRI) has been successfully utilized as a non-invasive imaging method in breast and liver cancer animal models [20]–[22]. With post-mortem histological analysis being the gold standard for EAC, our objective was to examine whether MRI would prove to be a valid tool to detect the presence of esophageal cancer in the Levrat model as compared to visual inspection with endoscopy.…”

Persistent T2*-hypointensity of the liver parenchyma after irradiation to the SPIO-accumulated liver: An imaging marker for responses to radiotherapy in hepatic malignancies

“…Room temperature and humidity were maintained at 22 ± 2 °C and 50%, respectively. After 1 week of acclimatization, 4 × 10 6 N1‐S1 hepatoma cells were inoculated into the left liver lobe as described previously . This N1‐S1 hepatoma model has been commonly used as a liver cancer animal model .…”

Magnetic Resonance-Based Visualization of Thermal Ablative Margins Around Hepatic Tumors by Means of Systemic Ferucarbotran Administration Before Radiofrequency Ablation

“…We inoculated N1-S1 hepatoma cells (CRL-1604; American Type Culture Collection, Manassas, VA; 4 Â 10 6 cells per head) into the left lateral lobes of the liver in 8 rats by following the technique described in the literature. 13,15,16 Seven days later, we acquired ferucarbotran-enhanced T2*-WIs of the liver (20-μmol iron /kg BW) and subsequently performed RFA in the hepatic tumor lesions by following the same technique as mentioned previously except for the length of electrode of 5 to 10 mm depending on tumor size. In addition, we carried out multi-RFA sessions for larger tumors.…”

“…13 Anesthesia was induced by inhalation of 5% isoflurane in the gas mixture of oxygen and nitrous oxide (1:1) and subsequently maintained with 1% to 2% isoflurane in the gas mixture. We then placed 2 to 4 rats separately on acrylic animal beds in either the prone or supine position; inserted the rats to the coil with their abdominal surfaces close to the coils; and simultaneously acquired transverse T2-weighted (T2WIs), T2*-weighted (T2*WIs), and proton-density-weighted images of the upper abdomen with the parameters as shown in Table 1.…”