Artichoke induces genetic toxicity in the cytokinesis-block micronucleus (CBMN) cytome assay

Jacociunas, Laura Vicedo; Andrade, Heloı́sa Helena Rodrigues de; Lehmann, Maurı́cio; Abreu, Bianca Regina Ribas de; Ferraz, Alexandre de Barros Falcão; Silva, Juliana da; Grivicich, Ivana; Dihl, Rafael Rodrigues

doi:10.1016/j.fct.2012.12.024

Cited by 19 publications

(10 citation statements)

References 29 publications

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“…The toxicity assessment is obligatory due to the potential toxic effects of compounds naturally present in the prepared infusions. 24 All infusions were prepared according to common practices. The concentrations of each component are shown in Table 2.…”

Section: Antioxidant Activity and Hepatotoxicitymentioning

confidence: 99%

Is honey able to potentiate the antioxidant and cytotoxic properties of medicinal plants consumed as infusions for hepatoprotective effects?

et al. 2015

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aDue to the enormous variety of phytochemicals present in plants, their extracts have been used for centuries in the treatment of innumerable diseases, being perceived as an invaluable source of medicines for humans. Furthermore, the combination of different plants was reported as inducing an improved effect (synergism) in comparison with the additive activity of the plants present in those mixtures. Nevertheless, information regarding the effects of plant infusions added with honey is still rather scarce. Accordingly, the aim of this study was to evaluate the interaction between chestnut honey, a natural product with wellreported beneficial properties, and three medicinal plants (either as a single plant or as combinations of two and three plants), with regard to their antioxidant activity and hepatotoxicity. Antioxidant activity was evaluated by comparing the results from four different assays; hepatotoxicity was assessed in two different cell lines. Results were compared by analysis of variance and linear discriminant analysis. The addition of honey to the infusions had a beneficial result in both cases, producing a synergistic effect in all samples, except β-carotene bleaching inhibition for artichoke + milk thistle + honey preparation and also preparations with lower hepatotoxicity, except in the case of artichoke + honey. Moreover, from the discriminant linear analysis output, it became obvious that the effect of honey addition overcame that resulting from using single plant or mixed plant based infusions. Also, the enhanced antioxidant activity of infusions containing honey was confirmed by lower hepatotoxicity.

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Section: Antioxidant Activity and Hepatotoxicitymentioning

confidence: 99%

Is honey able to potentiate the antioxidant and cytotoxic properties of medicinal plants consumed as infusions for hepatoprotective effects?

et al. 2015

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“…In fact, since some potential effects of compounds naturally in plants are difficult to anticipate, the assessment of the safety of a plant extract used as a food or a medicine by the population is completely mandatory. 9 Three plant species, namely artichoke (A), borututu (B) and milk thistle (M), which are commonly present in nutraceutical formulations/dietary supplements, were selected due to their availability in different formulations. Besides aiming studying the inuence of the formulation type on the bioactivity and phenolic compound content, this study was designed also to evaluate supposed differences resulting from using different percentages of the plant species in each formulation.…”

Section: Antioxidant Properties and Cytotoxicity For Hepatocellular T...mentioning

confidence: 99%

“…7 The genetic, cultural and environmental factors that explain this variability make their use rather challenging and frequently problematic because the active principles are diverse and may be unknown. 7,8 In some cases, these effects might even be harmful; for instance, the leaf extracts of artichoke caused chromosomal instability and cytotoxicity in hamster ovary cells, 9 while milk thistle extracts, at 15 µg/mL, showed toxicity against the activity of hepatic P450 cytochrome. 10 The consumption of supplements to provide the beneficial effects of certain plants has raised several controversial questions, such as those pointed out by Halliwell, who stated that "we cannot just pull out one or two individual molecules and expect pills containing high doses of them to protect us", 11 suggesting the whole herbal medicine as one active ingredient, i.e., a set of multi-component parts self-organized into an indivisible whole.…”

Section: Introductionmentioning

confidence: 99%

New insights into the effects of formulation type and compositional mixtures on the antioxidant and cytotoxic activities of dietary supplements based-on hepatoprotective plants

et al. 2014

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Artichoke (A), borututu (B) and milk thistle (M) are included in several supplements to provide beneficial effects. Different formulations (infusions, pills and syrups), with different proportions of A, B and M (1 : 1 : 1, 2 : 1 : 1, 1 : 2: 1, 1 : 1 : 2) within each formulation, were assayed to optimize the desired benefits. The antioxidant activity, anti-hepatocellular carcinoma activity, hepatotoxicity and bioactive compound contents were evaluated. Syrups tended to be the formulation with highest antioxidant activity and total phenolic and flavonoid content; otherwise, pills were the worst formulation. In what concerns A : B : M ratios, the results did not reveal so pronounced differences. None of the assayed mixtures resulted to be toxic (up to the maximum assayed dose) for liver primary cells (PLP2), but some samples, especially infusions, showed toxicity for the hepatocellular carcinoma cell line (HepG2). With no exception, the mixtures for all formulations showed synergistic effects on antioxidant activity, when compared to the activity of single plants.

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“…In contrast, although ECC is not mutagenic in Salmonella typhimurium TA98, it significantly increased the mutagenic effect of 2-aminofluorene and enhanced the cytotoxic/cytostatic effect of cis-Pt (Miadokova et al , 2006). We have also recently demonstrated the genotoxic and antigenotoxic potential of C. scolymus L. leaf extract in vitro (Jacociunas et al , 2012, 2013). …”

Section: Introductionmentioning

confidence: 97%

Effects of artichoke (Cynara scolymus) leaf and bloom head extracts on chemically induced DNA lesions in Drosophila melanogaster

et al. 2014

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The genotoxicity of bloom head (BHE) and leaf (LE) extracts from artichoke (Cynara scolymus L.), and their ability to modulate the mutagenicity and recombinogenicity of two alkylating agents (ethyl methanesulfonate – EMS and mitomycin C – MMC) and the intercalating agent bleomycin (BLM), were examined using the somatic mutation and recombination test (SMART) in Drosophila melanogaster. Neither the mutagenicity nor the recombinogenicity of BLM or MMC was modified by co- or post-treatment with BHE or LE. In contrast, co-treatment with BHE significantly enhanced the EMS-induced genotoxicity involving mutagenic and/or recombinant events. Co-treatment with LE did not alter the genotoxicity of EMS whereas post-treatment with the highest dose of LE significantly increased this genotoxicity. This enhancement included a synergistic increase restricted to somatic recombination. These results show that artichoke extracts promote homologous recombination in proliferative cells of D. melanogaster.

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Artichoke induces genetic toxicity in the cytokinesis-block micronucleus (CBMN) cytome assay

Cited by 19 publications

References 29 publications

Is honey able to potentiate the antioxidant and cytotoxic properties of medicinal plants consumed as infusions for hepatoprotective effects?

Is honey able to potentiate the antioxidant and cytotoxic properties of medicinal plants consumed as infusions for hepatoprotective effects?

New insights into the effects of formulation type and compositional mixtures on the antioxidant and cytotoxic activities of dietary supplements based-on hepatoprotective plants

Effects of artichoke (Cynara scolymus) leaf and bloom head extracts on chemically induced DNA lesions in Drosophila melanogaster

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