Arthus type inflammation with rat immunoglobulins

Tolone, G.; Bonasera, L.; Bruno, Roberto

doi:10.1007/bf02154298

Cited by 1 publication

(1 citation statement)

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“…An attempt to define the molecular class(es) of antibody affected is in progress. However, the Arthus reactions measured in the present study may be presumed to represent slow IgG, probably IgGa (12,13), the formation of which was obviously suppressed in appropriately treated animals.…”

Section: Figs 1-5 Suppression Of Immune Reactivity In Recipients Ofmentioning

confidence: 43%

The Thymic Suppressor Cell

Waksman

Treffers

1974

The Journal of Experimental Medicine

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We showed in an earlier study that thymocytes, taken from Lewis rats within a few days after a large systemic dose of bovine "),-globulin (BGG) 1 and transferred alive to normal syngeneic recipients, inhibit both antibody formation and cell-mediated immunity when the recipient is subsequently challenged with the same antigen in complete Freund's adjuvant (1). The inhibition is specific and partial rather than total, and it affects functions normally ascribed to both B and T lymphocytes. It appears to represent one of the rapidly growing groups of phenomena ascribed to "suppressor T cells" (reviewed in references 1 and 2). The present paper describes certain properties of the thymocyte subpopulation responsible for the observed inhibition. Materials and MethodsProtocoL--Adult, male Lewis rats (Microbiological Associates, Bethesda, Md.) (donors) received a single intraperitoneal dose of 100 mg of BGG (Cohn fraction II, Mann Research Laboratories, New York) or the control antigen OA (crystalline hen ovalbumin, Nutritional Biochemicals Corp., Cleveland, Ohio). In one group of experiments, 25 mg of hydrocortisone acetate (Nutritional Biochemicals Corp.) was injected intramuscularly at the same time.At 2 days, thymocytes were harvested by mincing donor thymus, gently squeezing the fragments between sterile slides in ice-cold medium (M199, prepared by the Laboratory of Epidemiology and Public Health, Yale University), expelling several times through a 23-gauge needle, filtering through nylon mesh, and finally washing five times with medium. Viability was usually greater than 90%. The cells from three donors were then injected intravenously into a single normal syngeneic recipient or were separated on density gradients (see below) and the subpopulations so obtained were injected into separate recipients.Recipients were challenged with 10 #g of BGG in complete Freund adjuvant in a footpad 24 h after cell transfer, were bled and skin tested with 30 pg of BGG and 30 pg of PPD (Parke, Davis and Co., Detroit, Mich.) 10 and 20 days after challenge, received an intraperitoneal booster dose of 1 mg of BGG at 25 days, and were subjected to a final bleeding at 32 days.

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Section: Figs 1-5 Suppression Of Immune Reactivity In Recipients Ofmentioning

confidence: 43%