Arthropoda: Pattern Recognition Proteins in Crustacean Immunity

Cerenius, Lage; Söderhäll, Kenneth

doi:10.1007/978-3-319-76768-0_10

Cited by 9 publications

(10 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(1,3)-glucan, which binds to the specific GNBP located on the haemocyte cell membrane [17]. The GNBPs play an essential role in the activation of proPO cascade [66]. Our findings indicate the expression of proPO in both susceptible and resistant crayfish can be altered in response to pathogen stimulation.…”

Section: Activation Of Prophenoloxidase Cascadementioning

confidence: 74%

Comparative transcriptome analysis of noble crayfish and marbled crayfish immune response to Aphanomyces astaci challenges

Boštjančić

Francesconi

Rutz

et al. 2021

Preprint

View full text Add to dashboard Cite

Introduction of invasive North American crayfish species and their pathogen Aphanomyces astaci has significantly contributed to the decline of European freshwater crayfish populations. In this study, noble crayfish, a susceptible native European species, and marbled crayfish, an invasive disease-resistant species, were challenged with haplogroup A (low virulence) and haplogroup B (high virulence) strain of A. astaci. Hepatopancreatic tissue was isolated 3 and 21 days post-challenge. Our results revealed strong up-regulation in expression levels of the prophenoloxidase cascade immune-related genes in the haplogroup B challenged noble crayfish 3 days post-challenge. In the marbled crayfish, we observed an up-regulation of immune system relevant genes (DSCAM, AP, ALFs, CTLs and hemocyanin) 3 days post-challenge. This response highlights the marbled crayfish capability of building the immune tolerance. Furthermore, we successfully characterised several novel immune related gene groups in both crayfish species, contributing to our current understanding of crayfish immune related genes landscape.

show abstract

Section: Activation Of Prophenoloxidase Cascadementioning

confidence: 74%

Comparative transcriptome analysis of noble crayfish and marbled crayfish immune response to Aphanomyces astaci challenges

Boštjančić

Francesconi

Rutz

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Lack of acquired (specific) immune response in crayfish is due to the lack of myeloid lineage cells being able to produce immunoglobulins (specific antibodies) against specific pathogens in the event of repetitive infections (lack of memory). This lack of acquired immune response was found to be balanced by the enrichment of different cell-mediated strategies and several multi-type humoral factors, both constituting the innate defenses of the crayfish immunity ( Figure 1 ) [e.g., multiple pathogen recognition receptors (PRRs)] ( 28 , 42 ). These defenses are activated in a cooperative way in order to initiate effective defensive actions and can be classified according to two main responses: a humoral-mediated response involving humoral factors produced and released by the immune cells (antimicrobial peptides, hydrolytic enzymes, and molecules of the ProPO system, etc.…”

Section: General Description Of Crayfish Immunitymentioning

confidence: 99%

“… Schematic presentation of crayfish immune response. Presentation of the main constituents of crayfish immune response alongside the cooperative action of the cellular-mediated and humoral-mediated responses and their corresponding factors ( 4 , 5 , 14 , 17 , 18 , 26 , 28 – 30 , 42 – 54 ). …”

Section: General Description Of Crayfish Immunitymentioning

confidence: 99%

“…These aim to neutralize the pathogen and prevent it from disseminating the hemocel ( 16 , 21 , 26 , 98 ). Overall, inflammation consists of several successive steps starting with the recognition step of pathogens and/or sensing tissue damages ( 16 , 17 , 28 , 30 ), followed by the recruitment of immune cells to the infected/damaged site, where additional effector molecules, such as clotting proteins (e.g., peroxinectins and transglutaminase), would be generated and released by these cells to consolidate pathogen isolation (i.e., capsule formation) ( 4 , 14 , 26 , 84 , 85 , 88 , 92 , 99 , 100 ) ( Figure 3 ). These activities may also initiate further recruitment of neo-formed hemocytes through the secretion of cytokine-like proteins (Astakine 1 and 2), which stimulate stem cell proliferation and new hemocyte maturation ( 83 , 85 ).…”

Section: Integration Of the Crayfish’ Immune Components Into Functional Mechanismsmentioning

confidence: 99%

See 1 more Smart Citation

A Comprehensive Review on Crustaceans’ Immune System With a Focus on Freshwater Crayfish in Relation to Crayfish Plague Disease

Bouallegui

2021

Front. Immunol.

View full text Add to dashboard Cite

Freshwater crayfish immunity has received great attention due to the need for urgent conservation. This concern has increased the understanding of the cellular and humoral defense systems, although the regulatory mechanisms involved in these processes need updating. There are, however, aspects of the immune response that require clarification and integration. The particular issues addressed in this review include an overall description of the oomycete Aphanomyces astaci, the causative agent of the pandemic plague disease, which affects freshwater crayfish, and an overview of crustaceans’ immunity with a focus on freshwater crayfish. It includes a classification system of hemocyte sub-types, the molecular factors involved in hematopoiesis and the differential role of the hemocyte subpopulations in cell-mediated responses, including hemocyte infiltration, inflammation, encapsulation and the link with the extracellular trap cell death pathway (ETosis). In addition, other topics discussed include the identity and functions of hyaline cells, the generation of neoplasia, and the emerging topic of the role of sessile hemocytes in peripheral immunity. Finally, attention is paid to the molecular execution of the immune response, from recognition by the pattern recognition receptors (PRRs), the role of the signaling network in propagating and maintaining the immune signals, to the effector elements such as the putative function of the Down syndrome adhesion molecules (Dscam) in innate immune memory.

show abstract

“…With the rst identi cation of a complement homolog found in the sea urchin in 1996 [60]. The recognition molecules and associated serine proteases, colins, MBL-associated serine proteases (MASPs), C1q1, CaC1q2, C3 and C2/Complement factor B (Bf) and C1q-domain containing protein have been recently reported in invertebrates [61][62][63][64][65][66]. In innate immunity, C1q-domain containing proteins can be considered as specialized PRPs because they have the ability to bind pathogens directly through PAMPs and to trigger phagocytosis [67].…”

Section: Complementmentioning

confidence: 99%

Immune Transcripts of the Pacific Oyster, Crassostrea gigas Larvae that Change in Expression as a Result of Bacteria Challenge

Fang¹,

Cai²,

Mao³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Marine molluscs living in intertidal and estuarine areas, such as oysters, face numerous pathogen challenges during their development. Infection from bacteria such as Vibrio alginolyticus, represents a major factor affecting larval development and frequently leads to high mortality of the pacific oyster, Crassostrea gigas. The oyster immune response is known to play an important role in protecting the animal during development by mitigating the consequences of infection. Results: In this study, we undertook a comprehensive analysis of the immune response of C. gigas to V. alginolyticus challenge. We sequenced the transcriptome of C. gigas at 0, 6, 12, 24, 48 and 72 hours post infection. After RNA-seq, the raw reads are available through the NCBI Sequence Read Archive under accession number PRJNA623063. After filtering, a total of 58.24 Gb clean reads were produced and assembled using the reference genome of C. gigas. The distribution of quality Q30 was higher than 90.88% for each sample and the GC content ranged from 41.27% to 42.91%. When compared with sequences in the COG, GO, KEGG, Swiss-Prot, and NR databases, there were 1267, 1112, 2187, 682, 1133 differentially expressed genes annotated at 6, 12, 24, 48, 72 hours post infection respectively. Numerous immune-related genes displayed differential expression that varied over time: toll-like receptors, tripartite motif proteins, Lectin-like factors, scavenger receptors, signaling pathway components such as Myeloid differentiation factor 88, and stress proteins such as Heat shock 70 kDa protein were all found to be higher in abundance following V. alginolyticus challenge compared to control. For analysis, these genes were divided into several categories such as pattern recognition receptors, fibrinogen-like proteins, damage associated molecular patterns, complement factors, etc. These general categories allowed us to generate an immune response profile for C. gigas over the first 72 hours of infection. These results indicate that bacterial infection induce a complex pattern of immune gene expression in C. gigas larvae. Conclusion: Our study will facilitate targeted investigation into the function of specific immune factors that may explain the diversity and evolution of invertebrate immune molecules and lead to the development of effective measures to improve the performance of oyster culture.

show abstract

Arthropoda: Pattern Recognition Proteins in Crustacean Immunity

Cited by 9 publications

References 74 publications

Comparative transcriptome analysis of noble crayfish and marbled crayfish immune response to Aphanomyces astaci challenges

Comparative transcriptome analysis of noble crayfish and marbled crayfish immune response to Aphanomyces astaci challenges

A Comprehensive Review on Crustaceans’ Immune System With a Focus on Freshwater Crayfish in Relation to Crayfish Plague Disease

Immune Transcripts of the Pacific Oyster, Crassostrea gigas Larvae that Change in Expression as a Result of Bacteria Challenge

Contact Info

Product

Resources

About