Arthropod Allergens in Urban Homes

Prester, Ljerka

doi:10.2478/10004-1254-63-2012-2125

Archives of Industrial Hygiene and Toxicology

2012

DOI: 10.2478/10004-1254-63-2012-2125

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Arthropod Allergens in Urban Homes

Ljerka Prester¹

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2012

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Comparison of buffers for extraction of mite allergen der p 1 from dust

Prester

Kovačić

Macan

2012

Archives of Industrial Hygiene and Toxicology

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Der p 1 is the main allergen of house dust mite Dermatophagoides pteronyssinus, which has routinely been detected in residential dust. However, the procedure for extracting Der p 1 from reservoir dust has not been well defi ned. The aim of this study was to compare Der p 1 mass fractions in dust extracts prepared using the following extraction buffers: phosphate (pH 7.4), borate (pH 8.0), and ammonium bicarbonate (pH 8.0), all with 0.05 % Tween 20. Twenty-eight dust samples were divided into three aliquots and each portion was extracted with one of the three buffers at room temperature. Der p 1 mass fractions were measured in a total of 84 dust extracts using the enzyme immunoassay (range: 0.1 μg g -1 to 7.53 μg g -1). Statistical methods including intraclass correlation showed a high agreement between Der p 1 mass fractions irrespective of the extracting medium. Our results suggest that all three buffers are suitable for the extraction of mite allergens and routine Der p 1 analysis in dust.

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Comparison of buffers for extraction of mite allergen der p 1 from dust

Prester

Kovačić

Macan

2012

Archives of Industrial Hygiene and Toxicology

Self Cite

View full text Add to dashboard Cite

show abstract

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Arthropod Allergens in Urban Homes

Cited by 1 publication

References 78 publications

Comparison of buffers for extraction of mite allergen der p 1 from dust

Comparison of buffers for extraction of mite allergen der p 1 from dust

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