Arthrobacter mobilis sp. nov., a novel actinobacterium isolated from Cholistan desert soil

Abstract

A Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-mycelium-forming, motile, rod-shaped with one polar flagellum actinobacterium, designated E918T, was isolated from a desert soil collected in Cholistan desert, Pakistan. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E918T belonged to the genus Arthrobacter and was most closely related to Arthrobacter deserti CGMCC 1.15091T (97.2 % similarity). The peptidoglycan was of the A3α type and the whole-cell sugar profile was found to contain galactose. The major menaquinone was MK-9(H2). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two unidentified glycolipids. The major fatty acids identified were anteiso-C15 : 0 and anteiso-C17 : 0. The G+C content of the genomic DNA was 68.69 mol%. The digital DNA–DNA hybridization and average nucleotide identity values between strain E918T and A. deserti CGMCC 1.15091T were 28.0 and 83.4%, respectively. On the basis of its phylogenetic, phenotypic and chemotaxonomic features, strain E918T was considered to represent a novel species of the genus Arthrobacter , for which the name Arthrobacter mobilis sp. nov. is proposed. The type strain of Arthrobacter mobilis is E918T (=JCM 33392T=CGMCC 1.16978T).

Culturing the desert microbiota

“…The most frequent species ( Supplementary Table 9 ) were A. subterraneus , whose type strain was isolated from the deep subsurface waters ( Chang et al, 2007 ), A. tecti (type strain isolated from mural paintings) ( Heyrman et al, 2005 ) and P. phenanthrenivorans (type strain isolated from creosote-contaminated soil) ( Kallimanis et al, 2009 ). Three species of Arthrobacter have recently been described from desert soils, Arthrobacter liuii isolated from Xinjiang desert soil, China ( Yu et al, 2015 ), Arthrobacter deserti isolated from Turpan desert soil, China ( Hu et al, 2016 ), and Arthrobacter mobilis isolated from Cholistan desert soil, Pakistan ( Ye et al, 2020 ), all of which were absent in the three Algerian sites. The presence of Arthrobacter species has also been evidenced in the Merzouga dune (Morocco) using culture-dependent and -independent approaches ( Gommeaux et al, 2010 ).…”

Ecology and resistance to UV light and antibiotics of microbial communities on UV cabins in the dermatology service of a Spanish hospital

“…have been isolated from polar environments and reported to be resistant to radiation 41 – 44 , whereas Arthrobacter spp. are present in hot deserts 2 , 45 , 46 . Moreover, Bacillus spp.…”

Arthrobacter zhaoxinii sp. nov. and Arthrobacter jinronghuae sp. nov., isolated from Marmota himalayana

“…Species in this genus are Gram-positive, aerobic, possess a high DNA G+C content and display a rod–coccus morphological cycle. They contain MK-9(H 2 ) or MK-8/MK-9 as the predominant menaquinone and iso-C 15 : 0 , anteiso-C 15 : 0 , and anteiso-C 17 : 0 as the major cellular fatty acids (CFAs) [14, 15]. Members in the genus Arthrobacter are mainly isolated from various environments (sewage, soil, water, ice, rhizosphere, marsh, and sediment), with a minority from animals or clinical specimens [10–12, 16, 17].…”