Arthritis biosensing: Aptamer-antibody-mediated identification of biomarkers by ELISA

Gopinath, Subash C.B.; Subramaniam, Sreeramanan; Wu, Zaifeng

doi:10.1016/j.procbio.2022.07.022

Cited by 6 publications

(5 citation statements)

References 29 publications

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“…Standard techniques for detection of IL-6 are immunoassays, Western blotting, mass spectroscopy, flow cytometry, and semiquantitative immunohistochemical detection . Of these, immunosorbent assays offer the lowest limits of IL-6 detection in human plasma, as low as 0.5 pg/mL. , However, these techniques require long incubation periods, trained personnel, and proper instrumentation, making them incompatible for routine clinical diagnosis, frequent testing, and monitoring. , To mitigate these limitations, immunosensing antibody-based nanosensors have emerged as a user-friendly point-of-care alternative . For example, nanosensors for IL-6 based on silica nanowires and metal nanoparticles have been reported. − …”

Section: Introductionmentioning

confidence: 99%

“… 32 Of these, immunosorbent assays offer the lowest limits of IL-6 detection in human plasma, as low as 0.5 pg/mL. 33 , 34 However, these techniques require long incubation periods, trained personnel, and proper instrumentation, making them incompatible for routine clinical diagnosis, frequent testing, and monitoring. 35 , 36 To mitigate these limitations, immunosensing antibody-based nanosensors have emerged as a user-friendly point-of-care alternative.…”

Section: Introductionmentioning

confidence: 99%

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Optical Nanosensor Passivation Enables Highly Sensitive Detection of the Inflammatory Cytokine Interleukin-6

Gaikwad,

Rahman,

Parikh

et al. 2024

ACS Appl. Mater. Interfaces

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Interleukin-6 (IL-6) is known to play a critical role in the progression of inflammatory diseases such as cardiovascular disease, cancer, sepsis, viral infection, neurological disease, and autoimmune diseases. Emerging diagnostic and prognostic tools, such as optical nanosensors, experience challenges in translation to the clinic in part due to protein corona formation, dampening their selectivity and sensitivity. To address this problem, we explored the rational screening of several classes of biomolecules to be employed as agents in noncovalent surface passivation as a strategy to screen interference from nonspecific proteins. Findings from this screening were applied to the detection of IL-6 by a fluorescent-antibody-conjugated single-walled carbon nanotube (SWCNT)-based nanosensor. The IL-6 nanosensor exhibited highly sensitive and specific detection after passivation with a polymer, poly-l-lysine, as demonstrated by IL-6 detection in human serum within a clinically relevant range of 25 to 25,000 pg/mL, exhibiting a limit of detection over 3 orders of magnitude lower than prior antibody-conjugated SWCNT sensors. This work holds potential for the rapid and highly sensitive detection of IL-6 in clinical settings with future application to other cytokines or disease-specific biomarkers.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optical Nanosensor Passivation Enables Highly Sensitive Detection of the Inflammatory Cytokine Interleukin-6

Gaikwad,

Rahman,

Parikh

et al. 2024

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…Standard techniques for detection of IL-6 are immunoassays, western blotting, mass spectroscopy, flow cytometry 42 , and semi-quantitative immunohistochemical detection 43 . Of these, immunosorbent assays offer the lowest limits of IL-6 detection in human plasma, as low as 0.5 pg/mL 44,45 . However, these techniques require long incubation periods, trained personnel, and proper instrumentation, making them incompatible for routine clinical diagnosis, frequent testing, and monitoring 46,47 .…”

Section: Introductionmentioning

confidence: 99%

Optical nanosensor passivation enables highly sensitive detection of the inflammatory cytokine IL-6

Gaikwad

Rahman

Parikh

et al. 2023

Preprint

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Interleukin-6 (IL-6) is known to a play critical role in the progression of inflammatory diseases such as cardiovascular disease, cancer, sepsis, viral infection, neurological disease, and autoimmune diseases. Emerging diagnostic and prognostic tools, such as optical nanosensors, experience challenges in successful clinical application in part due to protein corona formation dampening their selectivity and sensitivity. To address this problem, we explored the rational screening of several classes of biomolecules to be employed as agents in non-covalent surface passivation as a strategy to screen interference from non-specific proteins. Findings from this screening were applied to the detection of IL-6 by a fluorescent antibody-conjugated single-walled carbon nanotube (SWCNT)-based nanosensor. The IL-6 nanosensor exhibited highly sensitive and specific detection after passivation with a polymer, poly-L-lysine, as demonstrated by IL-6 detection in human serum within a clinically relevant range of 25 pg/mL to 25,000 pg/mL, exhibiting a limit of detection over three orders of magnitude lower than prior antibody-conjugated SWCNT sensors. This work holds the potential for rapid and highly sensitive detection of IL-6 in clinical settings with future application to other cytokines or disease-specific biomarkers.

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“…Researchers are focusing on the quantification of bloodbased biomarkers for early osteoporosis diagnosis, which helps to manage the better management of treatment and prevention. [7][8][9] Various biomarkers have been investigated, including the biomarkers for bone resorption, bone formation, and bone turnover. 10,11 These biomarkers are helpful for early identification of osteoporosis, and the imaging technique does not give enough information to make a decision.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, the alternative method of the detection system is mandatory for osteoporosis identification. Researchers are focusing on the quantification of blood‐based biomarkers for early osteoporosis diagnosis, which helps to manage the better management of treatment and prevention 7–9 . Various biomarkers have been investigated, including the biomarkers for bone resorption, bone formation, and bone turnover 10,11 .…”

Section: Introductionmentioning

confidence: 99%

High‐sensitivity immunoassay on interdigitated electrode to detect osteoporosis biological marker

Liu,

Chen,

et al. 2023

Biotech and App Biochem

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Osteoporosis is with porous bones, which refers to a decrease in the bone mineral density and weakens the bones to become brittle. Osteoporosis often progresses without any pain or symptoms until the bone fractures. Monitoring the condition of bone regularly helps to identify the bone that weakens at its earlier stages. In general, radiological techniques have been used to measure bone mineral density, are expensive, and the procedures are complicated. Therefore, researchers are focusing on the alternative method of biomarker quantification to identify bone mineral density. This research work was focused on quantifying the osteoporosis biomarker of C‐terminal telopeptide of type I collagen (CTX‐I) on an interdigitated electrode (IDE) sensor. Gold nanomaterial‐modified anti‐CTX‐I antibody was attached to silica nanomaterial‐decorated IDE and then identified by CTX‐I interaction. Higher immobilization of antibodies was recorded on diamond‐modified IDE through gold nanoparticles, and detected CTX‐I as low as 0.5 pg/mL [y= 1.5507x − 0.9043R2= 0.9715], determined on a linear curve at the range 0.5–3.5 ng/mL. Further, specific identification of CTX‐I was confirmed by control performances with osteopontin, IL‐6, and anti‐IgG antibody.

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Arthritis biosensing: Aptamer-antibody-mediated identification of biomarkers by ELISA

Cited by 6 publications

References 29 publications

Optical Nanosensor Passivation Enables Highly Sensitive Detection of the Inflammatory Cytokine Interleukin-6

Optical Nanosensor Passivation Enables Highly Sensitive Detection of the Inflammatory Cytokine Interleukin-6

Optical nanosensor passivation enables highly sensitive detection of the inflammatory cytokine IL-6

High‐sensitivity immunoassay on interdigitated electrode to detect osteoporosis biological marker

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