Arteriolar vascular smooth muscle cell differentiation in benign nephrosclerosis

Bockmeyer, Clemens L.; Kern, David Sebastian; Forstmeier, Vinzent; Lovric, Svjetlana; Modde, Friedrich; Agustian, Putri A.; Steffens, Sandra; Birschmann, Ingvíld; Traeder, Jana; Dämmrich, Maximilian Ernst; Schwarz, Anke; Kreipe, Hans; Bröcker, Verena; Becker, Jan U.

doi:10.1093/ndt/gfr811

Cited by 11 publications

(12 citation statements)

References 31 publications

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“…FSP1 has also been used as an early marker for epithelial-mesenchymal transition and may play important roles during fibrosis39404142. Arteriole SMCs were found to express FSP1 in benign nephrosclerosis samples and thought to contribute to fibrosis process43. In contrast to the traditional opinion that FSP1 expression is indicative of fibrosis, our results showed that FSP1 + cells still retain the capability of forming microvessels.…”

Section: Discussioncontrasting

confidence: 75%

“…However, there is evidence that the cells involved in these two processes may be related. For example, pericytes or perivascular progenitor cells171819 and SMCs43 have been reported to give rise to myofibroblasts during fibrosis. On the other hand, there has also speculation that myofibroblasts, when put in the right microenvironment, such as the media layer of large blood vessel walls, might differentiate into SMCs35, suggesting the possibility of reciprocal transformation of the two processes.…”

Section: Discussionmentioning

confidence: 99%

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Sox10+ adult stem cells contribute to biomaterial encapsulation and microvascularization

Wang

et al. 2017

Sci Rep

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Implanted biomaterials and biomedical devices generally induce foreign body reaction and end up with encapsulation by a dense avascular fibrous layer enriched in extracellular matrix. Fibroblasts/myofibroblasts are thought to be the major cell type involved in encapsulation, but it is unclear whether and how stem cells contribute to this process. Here we show, for the first time, that Sox10+ adult stem cells contribute to both encapsulation and microvessel formation. Sox10+ adult stem cells were found sparsely in the stroma of subcutaneous loose connective tissues. Upon subcutaneous biomaterial implantation, Sox10+ stem cells were activated and recruited to the biomaterial scaffold, and differentiated into fibroblasts and then myofibroblasts. This differentiation process from Sox10+ stem cells to myofibroblasts could be recapitulated in vitro. On the other hand, Sox10+ stem cells could differentiate into perivascular cells to stabilize newly formed microvessels. Sox10+ stem cells and endothelial cells in three-dimensional co-culture self-assembled into microvessels, and platelet-derived growth factor had chemotactic effect on Sox10+ stem cells. Transplanted Sox10+ stem cells differentiated into smooth muscle cells to stabilize functional microvessels. These findings demonstrate the critical role of adult stem cells in tissue remodeling and unravel the complexity of stem cell fate determination.

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Section: Discussioncontrasting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Sox10+ adult stem cells contribute to biomaterial encapsulation and microvascularization

Wang

et al. 2017

Sci Rep

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“…Cx43, which has been reported in fibroblasts (Zhang et al, 2008) and pericytes (Mogensen et al, 2011), was found in the pool of Nestin-GFP- cells, representing 10 ± 2.0 % of all cells in culture. The α-SMA marker, which has been found in vascular smooth muscle cells (Bockmeyer et al, 2012) and pericytes (Mogensen et al, 2011), is also present in Nestin-GFP-cells, accounting for 29 ± 5.8 % of all cells (Figs. 1A, B).…”

Section: Resultsmentioning

confidence: 99%

Skeletal muscle pericyte subtypes differ in their differentiation potential

et al. 2013

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Neural progenitor cells have been proposed as a therapy for central nervous system disorders, including neurodegenerative diseases and trauma injuries, however their accessibility is a major limitation. We recently isolated Tuj1+ cells from skeletal muscle culture of Nestin-GFP transgenic mice however whether they form functional neurons in the brain is not yet known. Additionally, their isolation from nontransgenic species and identification of their ancestors is unknown. This gap of knowledge precludes us from studying their role as a valuable alternative to neural progenitors. Here, we identified two pericyte subtypes, type-1 and type-2, using a double transgenic Nestin-GFP/NG2-DsRed mouse and demonstrated that Nestin-GFP+/Tuj1+ cells derive from type-2 Nestin-GFP+/NG2-DsRed+/CD146+ pericytes located in the skeletal muscle interstitium. These cells are bipotential as they generate either Tuj1+ cells when cultured with muscle cells or become “classical” α-SMA+ pericytes when cultured alone. In contrast, type-1 Nestin-GFP-/NG2-DsRed+/CD146+ pericytes generate α-SMA+ pericytes but not Tuj1+ cells. Interestingly, type-2 pericyte derived Tuj1+ cells retain some pericytic markers (CD146+/PDGFRβ+/NG2+). Given the potential application of Nestin-GFP+/NG2-DsRed+/Tuj1+ cells for cell therapy, we found a surface marker, the nerve growth factor receptor, which is expressed exclusively in these cells and can be used to identify and isolate them from mixed cell populations in nontransgenic species for clinical purposes.

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“…In the case of hyalinosis, the subendothelial presence of accumulated plasma proteins likely triggers the SMC response of dedifferentiation and synthesis of ECM proteins. 54,55 This concept is schematically depicted in Figure 9.…”

Section: Discussionmentioning

confidence: 99%

Klotho Deficiency Induces Arteriolar Hyalinosis in a Trade-Off with Vascular Calcification

Mencke

Umbach

Wiggenhauser

et al. 2019

The American Journal of Pathology

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Arteriolar vascular smooth muscle cell differentiation in benign nephrosclerosis

Cited by 11 publications

References 31 publications

Sox10+ adult stem cells contribute to biomaterial encapsulation and microvascularization

Sox10+ adult stem cells contribute to biomaterial encapsulation and microvascularization

Skeletal muscle pericyte subtypes differ in their differentiation potential

Klotho Deficiency Induces Arteriolar Hyalinosis in a Trade-Off with Vascular Calcification

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