Arterial walls generate from prostaglandin endoperoxides a substance (prostaglandin X) which relaxes strips of mesenteric and coeliac arteries and inhibits platelet aggregation

Bunting, Stuart; Moncada, Salvador; Vane, John R.; Gryglewski, R

doi:10.1016/0090-6980(76)90125-8

Cited by 917 publications

(288 citation statements)

References 24 publications

Supporting

Mentioning

273

Contrasting

Unclassified

Order By: Relevance

“…With positive chemical ionization mode, two major products were observed at 13.325 and 13.398 min, respectively. Mass spectra of the 13.325-min product revealed the presence of major ions of m/z 511 (M-CH 3 (Fig. 5B).…”

Section: Resultsmentioning

confidence: 99%

Identification of 15-hydroxy-11,12-epoxyeicosatrienoic acid as a vasoactive 15-lipoxygenase metabolite in rabbit aorta

Chawengsub

Aggarwal

Nithipatikom

et al. 2008

American Journal of Physiology-Heart and Circulatory Physiology

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Identification of 15-hydroxy-11,12-epoxyeicosatrienoic acid as a vasoactive 15-lipoxygenase metabolite in rabbit aorta

Chawengsub

Aggarwal

Nithipatikom

et al. 2008

American Journal of Physiology-Heart and Circulatory Physiology

View full text Add to dashboard Cite

show abstract

“…Aortic rings were prepared by methods similar to those described previously (Bunting et al, 1976;Ritter et al, 1982a,b group of aortic rings served as control (no added Ca2+), and the remaining 3 solutions contained different concentrations of Ca2+. The effect of Ca2+ was expressed as the ratio of 6-oxo-PGF,, produced in the presence to that produced in the absence of added Ca2+ ('fold stimulation').…”

Section: Aortic Ring Incubationsmentioning

confidence: 99%

“…Prostacyclin (PGI2) is synthesized by vascular tissue in response to trauma (Bunting et al, 1976). PGI2 has potent actions on platelets and vascular smooth muscle (Moncada & Vane, 1979), and local PGI2 synthesis by damaged blood vessels may be an important component of the vascular response to injury which is a process of major pathological significance (Ross, 1986).…”

Section: Introductionmentioning

confidence: 99%

pH‐dependent stimulation by Ca²⁺ of prostacyclin synthesis in rat aortic rings: effects of drugs and inorganic ions

Ritter

Frazer

Taylor

1987

British J Pharmacology

View full text Add to dashboard Cite

1 Fresh rat aortic rings were incubated in HEPES-buffered salt solutions. Extracellular Ca24 stimulated the production of prostacyclin (PGI2), as determined by radioimmunoassay of its stable hydrolysis product 6-oxo-prostaglandin F,. This action of Ca24 was modified by H4 over the pH range 8.0-6.5. Stimulation by calcium ionophore A23187 was not pH-dependent.2 In parallel incubations of aortic rings with 4 Ca2", followed by washing in the presence of La3, tissue uptake of 45Ca2" increased progressively as extracellular pH was increased from 6.5-8.0. Over this range intracellular pH, estimated by the distribution of ['4CJ-dimethadione, varied from 5.9-7.4.3 Stimulation by Ca24 of PGO2 synthesis was concentration-dependent over the range 0.7-20 mm. The maximum effect was an increase of approx. 4 fold. 4 Nifedipine, but not verapamil or diltiazem, inhibited Ca24-stimulated PGI2 synthesis. A dihydropyridine compound that activates voltage-dependent Ca24 channels, Bay K 8644, did not increase PGI2 synthesis. 5 8-(N, N-diethylamino)-octyl-3,4,5 timethoxybenzoate (TMB-8), an antagonist of intracellular Ca24 mobilisation, inhibited basal and Ca24-stimulated PGO2 synthesis to a similar extent. 6 A solution containing 40 mM K4 reduced Ca24-stimulated PGI2 production. Mg24 stimulated PGI2 synthesis in a pH-dependent manner but was less potent than Ca2 . Other divalent cations (Mn24, Ba24 and Sr`9), and La34 had little or no effect on basal or Ca2+-stimulated PGI2 synthesis.

show abstract

“…It is well established that prostacyclin has a potent inhibitory effect on platelet aggregation (Whittle et al, 1978) and also has vasodilator effects Bunting et al, 1976). Furthermore, cytoprotective effects of prostacyclin on various tissues, including those of the cardiovascular system have been suggested (Ogletree et al, 1979).…”

Section: Introduction Methodsmentioning

confidence: 99%

Effects of beraprost on the transmembrane potentials of guinea‐pig ventricular muscles during normoxia and hypoxia‐reoxygenation

Ueno

Shigenobu

Nishio

1993

British J Pharmacology

View full text Add to dashboard Cite

1 The present study was performed to determine whether beraprost, a new stable analogue of prostacyclin, may exert beneficial effects on the transmembrane action potentials during normoxia and hypoxia-reoxygenation in isolated right ventricular muscles of the guinea-pig.2 Under normal oxygenation, beraprost (0.01-100 imol'1) had no effects on the electrophysiological parameters. 3 Hypoxic conditions induced a decrease in action potential duration (APD) without affecting other action potential parameters. Beraprost inhibited this hypoxia-induced decrease in APD. However, beraprost had no effect on the decrease in contractile force induced by hypoxia, whereas it significantly improved the recovery of contractile force after reoxygenation. 4 Pinacidil-induced shortening of APD was not antagonized by beraprost. 5 Hypoxia significantly decreased the myocardial adenosine triphosphate (ATP) level, which was also prevented by beraprost. 6 These results suggested that beraprost may inhibit the hypoxia-induced shortening of APD by some mechanisms which contribute to the maintenance of muscle ATP level.

show abstract

Arterial walls generate from prostaglandin endoperoxides a substance (prostaglandin X) which relaxes strips of mesenteric and coeliac arteries and inhibits platelet aggregation

Cited by 917 publications

References 24 publications

Identification of 15-hydroxy-11,12-epoxyeicosatrienoic acid as a vasoactive 15-lipoxygenase metabolite in rabbit aorta

Identification of 15-hydroxy-11,12-epoxyeicosatrienoic acid as a vasoactive 15-lipoxygenase metabolite in rabbit aorta

pH‐dependent stimulation by Ca²⁺ of prostacyclin synthesis in rat aortic rings: effects of drugs and inorganic ions

Effects of beraprost on the transmembrane potentials of guinea‐pig ventricular muscles during normoxia and hypoxia‐reoxygenation

Contact Info

Product

Resources

About

Arterial walls generate from prostaglandin endoperoxides a substance (prostaglandin X) which relaxes strips of mesenteric and coeliac arteries and inhibits platelet aggregation

Cited by 917 publications

References 24 publications

Identification of 15-hydroxy-11,12-epoxyeicosatrienoic acid as a vasoactive 15-lipoxygenase metabolite in rabbit aorta

Identification of 15-hydroxy-11,12-epoxyeicosatrienoic acid as a vasoactive 15-lipoxygenase metabolite in rabbit aorta

pH‐dependent stimulation by Ca2+ of prostacyclin synthesis in rat aortic rings: effects of drugs and inorganic ions

Effects of beraprost on the transmembrane potentials of guinea‐pig ventricular muscles during normoxia and hypoxia‐reoxygenation

Contact Info

Product

Resources

About

pH‐dependent stimulation by Ca²⁺ of prostacyclin synthesis in rat aortic rings: effects of drugs and inorganic ions