“…Blood samples (approximately 5 mL) were centrifuged immediately after collection at 3000× g for 10 min. Serum levels of blood urea nitrogen, creatinine, fasting glucose, total cholesterol, triglycerides, low-density lipoprotein cholesterol (LDL-C), total calcium, phosphorus, and C-reactive protein (CRP) were measured using an autoanalyzer (Siemens Advia 1800; Siemens Healthcare GmbH, Henkestr, Germany) [21,22]. Human intact parathyroid hormone (iPTH) levels (Abcam, Cambridge, MA, USA) and 25-hydroxyvitamin D levels (Crystal Chem USA, Elk Grove Village, IL, USA) were measured using commercially available enzyme-linked immunosorbent assays.…”