Artemisia campestris leaf extract alleviates early diabetic nephropathy in rats by inhibiting protein oxidation and nitric oxide end products

Sefi, Mediha; Fetoui, Hamadi; Soudani, Nejla; Chtourou, Yassine; Makni, Mohamed; Zeghal, Najiba

doi:10.1016/j.prp.2012.01.002

Cited by 42 publications

(29 citation statements)

References 32 publications

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“…This extract had favorable effects on the diabetic rats, which received CO extract, and caused a significant decrease in the level of BUN and Cr in comparison to DC. In other similar studies on different plants, such favorable effects have been reported (3,30). The present study showed that STZ made pathological changes in the kidney tissue, which was inconsistent with the results of Nirmala et al (35).…”

Section: Discussioncontrasting

confidence: 99%

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Controversial effects of Calendula officinalis L. on Biochemical and Pathological Factors of Nephropathy in Diabetic Wistar Rats

Salehi¹,

Moradkhani²,

Roushandeh³

et al. 2015

Avicenna J Med Biochem

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Background: Chronic hyperglycemia leads to microvascular and macrovascular complications such as diabetic nephropathy. Medicinal plants are good sources for finding new therapeutic chemicals to improve diabetes and relieve its symptoms. Objectives: The purpose of the present study was to evaluate the effect of the hydroalcoholic extract (300 mg/kg) of Calendula officinalis (marigold) on blood biochemical profiles and histopathological changes in kidney of streptozotocin-induced diabetic rats. Materials and Methods: Twenty male Wistar rats were divided to four groups: Normal control (NC), diabetic control (DC), normal C. officinalis (N+CO) 300 mg/kg, and diabetic C. officinalis (D+CO) 300 mg/kg. The rats were treated for a period of 13 weeks. Diabetes was induced by Streptozocin injection, intraperitoneally. Level of glucose, urea, creatinine and also total anti-oxidant capacity, malondialdehyde, total oxidant status in serum and histological alterations in the kidney were analyzed. Results: Level of serum glucose, urea, creatinine, malondialdehyde and total oxidant status were increased in diabetic rats, whereas, total anti-oxidant capacity was decreased compared to the control animals. Also, histological findings confirmed the absence of integrity in glomerulus and mass infiltration in kidney tissue in diabetic rats compared to the normal controls. Calendula officinalis extract had no effect on blood glucose, but it decreased blood urea nitrogen and creatinine, total oxidant status and malondialdehyde while it increased total anti-oxidant capacity in the diabetic extract-treated group when compared to diabetic rats. Calendula officinalis could not prevent nephropathy changes in the diabetic rats. Conclusions: Therefore, our results suggest that although administration of 300 mg/kg of Calendula officinalis extract showed salutary effects on anti-oxidant profile, yet its protective effects on anti-diabetic and regenerative properties on nephropathy were ambiguous and require more investigations.

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Section: Discussioncontrasting

confidence: 99%

“…Thus induction of diabetes by STZ was successful. Expectedly, imbalance of oxidant/antioxidant system in diabetes caused oxidative stress and consequently, elevated level of MDA and TOS, and decreased TAC in the blood (26,30). The diabetic model, which was induced by STZ, also caused pathological changes in the kidney tissue.…”

Section: Discussionmentioning

confidence: 99%

Controversial effects of Calendula officinalis L. on Biochemical and Pathological Factors of Nephropathy in Diabetic Wistar Rats

Salehi¹,

Moradkhani²,

Roushandeh³

et al. 2015

Avicenna J Med Biochem

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“…19 In the present study proteins appeared in the urine DN rats. Oral administration of AVE significantly decreased the levels of urinary protein excretion that shows the development of renal functions.…”

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confidence: 56%

Evaluation of aloe vera leaves extract in streptozotocin- induced diabetic nephropathy in rat

Dangi

Gyanwali

Gyanwali³

et al. 2017

J Chitwan Med Coll

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Having an anti-diabetic activity, Aloe Vera (Aloebarbadensis) has been used for medicinal purposes in several cultures for millennia. It has also been proved that aloe vera is having protective activity in nephrotoxic rats.The objective of the present study was to evaluate the effect of Aloe vera leaves extract on diabetic nephropathy in Streptozotocin-induced diabetic rat. Diabetes was induced by Streptozotocin(60 mg/kg-i.p.). Four weeks later rats were randomly selected and divided into 5 groups (n=6). Group I: normal control. Group II: diabetic nephropathy (DN) control. Group III: DN+AVE (250 mg/ kg). Group IV: DN+AVE (500 mg/kg). Group V: DN+AMG (1 mg/1ml in drinking water). The treatment was given for 8 weeks. Various parameters like physiological, antidiabetic, renal function, antioxidants and histopathology were measured. Administration of aloe vera extract showed significant decrease in body weight, food and water intake, kidney weight, blood glucose level, serum creatinine, BUN, protein in urine, serum uric acid and lipid peroxidation. It also significantly increases the plasma insulin level, liver glycogen content, urine volume, GFR, SOD, catalase, Reduced glutathione. Histopathological studies confirmed that administration of AVE prevented kidney damage, which provided structural support for the renal shielding effect. The significant effect of aloe vera extract on diabetic nephropathy could be due to the inherent antihyperglycemic, antioxidant, improvement of renal function parameters. In the near future AVE could constitute a lead to the discovery of a novel drug for the treatment of diabetic nephropathy.

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“…Lipopolysaccharide increases the enzymatic activity and protein and mRNA levels of inducible nitric oxide synthase (iNOS) in MCs, suggesting that MCs are a potential source of NO production [15,16] . Reports from animal experiments show that increased renal NO production is one of the major pathogenic features of early DN [17][18][19] . However, the molecular mechanism for the effects of hyperglycemia on renal NO production remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Over-production of nitric oxide by oxidative stress-induced activation of the TGF-β1/PI3K/Akt pathway in mesangial cells cultured in high glucose

et al. 2013

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Aim: To investigate whether NO over-production in rat mesangial cells cultured in high glucose (HG) is related to activation of the TGF-β1/PI3K/Akt pathway. Methods: Rat mesangial cells line (HBZY-1) was exposed to HG (24.44 mmol/L) or H 2 O 2 (10 μmol/L) for 16 h. NO release was quantified using the Griess assay. The TGF-β1 level was measured using ELISA. The protein expression of p-Akt, t-Akt, Bim, and iNOS was examined by Western blotting. The mRNA levels of TGF-β1 and Bim were measured using RT-PCR. The cell proliferation rate was estimated using a BrdU incorporation assay. Results: Treatment of the cells with HG, H 2 O 2 , or TGF-β1 (5 ng/mL) significantly increased the NO level that was substantially inhibited by co-treatment with the NADPH oxidase inhibitor diphenylene iodonium (DPI), TGF-β1 inhibitor SB431542, or PI3K inhibitor LY294002. Both HG and H 2 O 2 significantly increased the protein and mRNA levels of TGF-β1 in the cells, and HG-induced increases of TGF-β1 protein and mRNA were blocked by co-treatment with DPI. Furthermore, the treatment with HG or H 2 O 2 significantly increased the expression of phosphorylated Akt and iNOS and cell proliferation rate, which was blocked by co-treatment with DPI, SB431542, or LY294002. Moreover, the treatment with HG or H 2 O 2 significantly inhibited Bim protein and mRNA expression, which was reversed by co-treatment with DPI, SB431542, or LY294002. Conclusion:The results demonstrate that high glucose causes oxidative stress and NO over-production in rat mesangial cells in vitro via decreasing Bim and increasing iNOS, which are at least partially mediated by the TGF-β1/PI3K/Akt pathway.

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Artemisia campestris leaf extract alleviates early diabetic nephropathy in rats by inhibiting protein oxidation and nitric oxide end products

Cited by 42 publications

References 32 publications

Controversial effects of Calendula officinalis L. on Biochemical and Pathological Factors of Nephropathy in Diabetic Wistar Rats

Controversial effects of Calendula officinalis L. on Biochemical and Pathological Factors of Nephropathy in Diabetic Wistar Rats

Evaluation of aloe vera leaves extract in streptozotocin- induced diabetic nephropathy in rat

Over-production of nitric oxide by oxidative stress-induced activation of the TGF-β1/PI3K/Akt pathway in mesangial cells cultured in high glucose

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