Artemisia annua L. and photoresponse: from artemisinin accumulation, volatile profile and anatomical modifications to gene expression

Lopes, Ellen M.; Guimarães-Dias, Fábia; Gama, Thália do Socorro Serra; Macedo, Arthur L.; Valverde, Alessandra L.; Moraes, Marcela Cristina de; Aguiar-Dias, Ana Cristina Andrade de; Bizzo, H. R.; Alves‐Ferreira, Márcio; Tavares, Eliana Schwartz; Macedo, Andrea Furtado

doi:10.1007/s00299-019-02476-0

Cited by 16 publications

(10 citation statements)

References 99 publications

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“…Improvements of bioactive compounds of A. annua under artificial light spectra were recently reported. Lopes et al [ 30 ] revealed increases in artemisinin content in A. annua when exposed to different light spectra. They determined that the increased content was induced by artificial light activation of the ADS gene in the cyclization steps of regulating transcriptomic artemisinin production.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies demonstrated that different light qualities impacted terpenoid variability. Red spectrum enhanced abundances of most sesquiterpenes, while blue spectrum induced greater relative abundance of monoterpenes [ 30 ]. A similar result was observed in tomato leaf, with monoterpene α-pinene more abundant under blue spectrum [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

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Variation in terpenoids in leaves of Artemisia annua grown under different LED spectra resulting in diverse antimalarial activities against Plasmodium falciparum

et al. 2022

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Background Productivities of bioactive compounds in high-value herbs and medicinal plants are often compromised by uncontrollable environmental parameters. Recent advances in the development of plant factories with artificial lighting (PFAL) have led to improved qualitative and/or quantitative production of bioactive compounds in several medicinal plants. However, information concerning the effect of light qualities on plant pharmaceutical properties is limited. The influence of three different light-emitting diode (LED) spectra on leaf fresh weight (FW), bioactive compound production and bioactivity of Artemisia annua L. against the malarial parasite Plasmodium falciparum NF54 was investigated. Correlation between the A. annua metabolites and antimalarial activity of light-treated plant extracts were also determined. Results Artemisia annua plants grown under white and blue spectra that intersected at 445 nm exhibited higher leaf FW and increased amounts of artemisinin and artemisinic acid, with enhanced production of several terpenoids displaying a variety of pharmacological activities. Conversely, the red spectrum led to diminished production of bioactive compounds and a distinct metabolite profile compared with other wavelengths. Crude extracts obtained from white and blue spectral treatments exhibited 2 times higher anti-Plasmodium falciparum activity than those subjected to the red treatment. Highest bioactivity was 4 times greater than those obtained from greenhouse-grown plants. Hierarchical cluster analysis (HCA) revealed a strong correlation between levels of several terpenoids and antimalarial activity, suggesting that these compounds might be involved in increasing antimalarial activity. Conclusions Results demonstrated a strategy to overcome the limitation of A. annua cultivation in Bangkok, Thailand. A specific LED spectrum that operated in a PFAL system promoted the accumulation of some useful phytochemicals in A. annua, leading to increased antimalarial activity. Therefore, the application of PFAL with appropriate light spectra showed promise as an alternative method for industrial production of A. annua or other useful medicinal plants with minimal environmental influence.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Variation in terpenoids in leaves of Artemisia annua grown under different LED spectra resulting in diverse antimalarial activities against Plasmodium falciparum

et al. 2022

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“…AS also participates in the process of antibiotic stress response ( Staiger and Brown, 2013 ; Filichkin et al, 2015 ). Plants produce secondary metabolites to defend against biological or abiotic stress, e.g., light, plant hormones, temperature, saline and drought stresses ( Li et al, 2007 ; Yu Z. X. et al, 2012 ; Shen et al, 2016 ; Vashisth et al, 2018 ; Lopes et al, 2019 ). Specifically, the expression of isoforms produced by IR splicing can be regulated depending on source of stress ( Filichkin et al, 2010 ; Abdel-Ghany et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

“…For example, light is an important environmental factor that regulates plant physiological adaptation and influences the synthesis of plant secondary metabolites in plant. Light regulation has been demonstrated to promote the accumulation of artemisinin, anthocyanins, ginsenoside and charantin ( Cuong et al, 2017 ; Kochan et al, 2019 ; Lopes et al, 2019 ; Sun et al, 2020 ). Light exposure immediately induced IR, with many alternatively spliced transcripts expressed from genes with functions related to light signaling, suggesting a potential impact on pre-mRNA splicing and photomorphogenic gene regulation in response to light ( Petrillo et al, 2014a , b ; Godoy Herz et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of Light-Regulated Alternative Splicing in Artemisia annua L.

Gao

Zhang

et al. 2021

Front. Plant Sci.

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Artemisinin is currently the most effective ingredient in the treatment of malaria, which is thus of great significance to study the genetic regulation of Artemisia annua. Alternative splicing (AS) is a regulatory process that increases the complexity of transcriptome and proteome. The most common mechanism of alternative splicing (AS) in plant is intron retention (IR). However, little is known about whether the IR isoforms produced by light play roles in regulating biosynthetic pathways. In this work we would explore how the level of AS in A. annua responds to light regulation. We obtained a new dataset of AS by analyzing full-length transcripts using both Illumina- and single molecule real-time (SMRT)-based RNA-seq as well as analyzing AS on various tissues. A total of 5,854 IR isoforms were identified, with IR accounting for the highest proportion (48.48%), affirming that IR is the most common mechanism of AS. We found that the number of up-regulated IR isoforms (1534/1378, blue and red light, respectively) was more than twice that of down-regulated (636/682) after treatment of blue or red light. In the artemisinin biosynthetic pathway, 10 genes produced 16 differentially expressed IR isoforms. This work demonstrated that the differential expression of IR isoforms induced by light has the potential to regulate sesquiterpenoid biosynthesis. This study also provides high accuracy full-length transcripts, which can be a valuable genetic resource for further research of A. annua, including areas of development, breeding, and biosynthesis of active compounds.

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“…[4][5][6][7][8][9][10][11] More papers are related to HS-SPME analysis some of which refer to A. scoparia 12 and A. annua. [13][14][15][16][17] Static headspace analysis of plants is a very fast and inexpensive method. Also, no special sample preparation is required, it could be performed even without a solvent and the conditions of analysis are not vigorous, so degradation of the components of the sample is minimized and the loss of the most volatile components is greatly reduced.…”

Section: Introductionmentioning

confidence: 99%

Composition of essential oils and headspace constituents of Artemisia annua L. and A. scoparia Waldst. et Kit.

Ickovski

Stepić

Stojanović

2020

JSCS

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Headspace volatiles (HS) and hydrodistilled essential oils (EO) of fresh aerial parts of Artemisia annua L. and A. scoparia Waldst. et Kit., were analyzed by GC-MS/FID. Artemisia ketone was found to be the most abundant component among the EO volatiles (55.8 %), as well as among HS (52.1 %) of A. annua. Additionally, in both A. annua samples, EO and HS, ?-pinene (12.7 and 24.2 %, respectively) was found in high percentage. On the other hand, it has been determined that the dominant components of A. scoparia EO and HS were different; in the essential oil capillene (63.8 %) was found as the main constituent, while ?-pinene (26.1 %), (Z)-?-ocimene (23.8 %) and limonene (10.7 %) were the major components among the HS. This is the first report on the composition of HS volatiles of the A. annua and A. scoparia obtained by direct static headspace. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 451-03-68/2020-14/200124]

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Artemisia annua L. and photoresponse: from artemisinin accumulation, volatile profile and anatomical modifications to gene expression

Cited by 16 publications

References 99 publications

Variation in terpenoids in leaves of Artemisia annua grown under different LED spectra resulting in diverse antimalarial activities against Plasmodium falciparum

Variation in terpenoids in leaves of Artemisia annua grown under different LED spectra resulting in diverse antimalarial activities against Plasmodium falciparum

Genome-Wide Analysis of Light-Regulated Alternative Splicing in Artemisia annua L.

Composition of essential oils and headspace constituents of Artemisia annua L. and A. scoparia Waldst. et Kit.

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