Arsenite response in <i>Coccomyxa</i> sp. Carn explored by transcriptomic and non‐targeted metabolomic approaches

Koechler, Sandrine; Bertin, Philippe; Plewniak, Frédéric; Baltenweck, Raymonde; Casiot, Corinne; Heipieper, Hermann J.; Bouchez, Olivier; Arsène-Ploetze, Florence; Hugueney, Philippe; Halter, David

doi:10.1111/1462-2920.13227

Cited by 20 publications

(9 citation statements)

References 32 publications

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“…Thus, the loss of organic acid-producing fermentation pathways by C. eustigma probably contributed to the adaptation to acidic environments with high concentrations of metals. This probably also accounts for the independent loss of fermentation genes from Co. subellipsoidea because the genus Coccomyxa contains several acidophilic members (29,30).…”

Section: Resultsmentioning

confidence: 99%

“…4 A and B). The genus Coccomyxa contains several acidophiles (29,30). However, it is currently unclear whether there is a correlation between the loss of organic acid-producing fermentation and adaptation to an acidic environment in acidophilic red algae, because they possess only the lactate fermentation pathway and alcohol dehydrogenases ( Fig.…”

Section: Discussionmentioning

confidence: 99%

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Acidophilic green algal genome provides insights into adaptation to an acidic environment

Hirooka

Hirose

Kanesaki

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

100

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Some microalgae are adapted to extremely acidic environments in which toxic metals are present at high levels. However, little is known about how acidophilic algae evolved from their respective neutrophilic ancestors by adapting to particular acidic environments. To gain insights into this issue, we determined the draft genome sequence of the acidophilic green alga and performed comparative genome and transcriptome analyses between and its neutrophilic relative The results revealed the following features in that probably contributed to the adaptation to an acidic environment. Genes encoding heat-shock proteins and plasma membrane H-ATPase are highly expressed in This species has also lost fermentation pathways that acidify the cytosol and has acquired an energy shuttle and buffering system and arsenic detoxification genes through horizontal gene transfer. Moreover, the arsenic detoxification genes have been multiplied in the genome. These features have also been found in other acidophilic green and red algae, suggesting the existence of common mechanisms in the adaptation to acidic environments.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Acidophilic green algal genome provides insights into adaptation to an acidic environment

Hirooka

Hirose

Kanesaki

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

100

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“…Representatives of the majority of these populations were also retrieved in our analysis of 16S rRNA reconstructed sequences from metagenomes with EMIRGE ( Figure 1). MAGs that were not captured by EMIRGE (e.g., MAGs 7,8,9,10,and 12) corresponded to low abundance (<1%) populations, which could explain why their full-length 16S rRNA sequences were not assembled from the metagenomic data. MAGs 8, 12, and 13 were not included in downstream analyses because less than 500 mRNA reads were mapped to their respective contigs (data not shown).…”

Section: Metal Resistance Mechanisms In Deep Layer Populationsmentioning

confidence: 99%

“…The As(III) efflux transporter, Acr3, was found in acidophilic green algae [11] such as Coccomyxa sp. Carn that transcribed genes homologous to acr3 when exposed to arsenic [12]. As(III) is considered to be more toxic than As(V) so its export is critical [13].…”

Section: Introductionmentioning

confidence: 99%

Metagenomic and Metatranscriptomic Study of Microbial Metal Resistance in an Acidic Pit Lake

et al. 2020

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Cueva de la Mora (CM) is an acidic, meromictic pit lake in the Iberian Pyrite Belt characterized by extremely high metal(loid) concentrations and strong gradients in oxygen, metal, and nutrient concentrations. We hypothesized that geochemical variations with depth would result in differences in community composition and in metal resistance strategies among active microbial populations. We also hypothesized that metal resistance gene (MRG) expression would correlate with toxicity levels for dissolved metal species in the lake. Water samples were collected in the upper oxic layer, chemocline, and deep anoxic layer of the lake for shotgun metagenomic and metatranscriptomic sequencing. Metagenomic analyses revealed dramatic differences in the composition of the microbial communities with depth, consistent with changing geochemistry. Based on relative abundance of taxa identified in each metagenome, Eukaryotes (predominantly Coccomyxa) dominated the upper layer, while Archaea (predominantly Thermoplasmatales) dominated the deep layer, and a combination of Bacteria and Eukaryotes were abundant at the chemocline. We compared metal resistance across communities using a curated list of protein-coding MRGs with KEGG Orthology identifiers (KOs) and found that there were broad differences in the metal resistance strategies (e.g., intracellular metal accumulation) expressed by Eukaryotes, Bacteria, and Archaea. Although normalized abundances of MRG and MRG expression were generally higher in the deep layer, expression of metal-specific genes was not strongly related to variations in specific metal concentrations, especially for Cu and As. We also compared MRG potential and expression in metagenome assembled genomes (MAGs) from the deep layer, where metal concentrations are highest. Consistent with previous work showing differences in metal resistance mechanisms even at the strain level, MRG expression patterns varied strongly among MAG populations from the same depth. Some MAG populations expressed very few MRG known to date, suggesting that novel metal resistance strategies remain to be discovered in uncultivated acidophiles.

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“…[2][3][4]15) Intracellular GSH plays an important role in antioxidant defense. [19][20][21] The present study shows that the α,β-unsaturated carbonyl compounds in CSE are rapidly taken up into sheep blood cells and react with intracellular GSH to produce the respective Michael adducts, which are promptly reduced to the corresponding alcohol forms and excreted into the extracellular fluid. In our previous paper, very similar results were reported on the metabolism of α,β-unsaturated carbonyl compounds in mouse melanoma (B16-BL6) cells exposed to CSE, although aldo-keto reductase activity in mouse melanoma cells was weaker than that in the blood cells.…”

Section: Discussionmentioning

confidence: 62%

Detoxification Mechanism of α,β-Unsaturated Carbonyl Compounds in Cigarette Smoke Observed in Sheep Erythrocytes

Horiyama

Hatai

Ichikawa

et al. 2018

CHEMICAL & PHARMACEUTICAL BULLETIN

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Highly reactive α,β-unsaturated carbonyl compounds, such as acrolein (ACR), crotonaldehyde (CA) and methyl vinyl ketone (MVK), are environmental pollutants present in high concentrations in cigarette smoke. We have previously found that these carbonyl compounds in cigarette smoke extract (CSE) react with intracellular glutathione (GSH) to produce the corresponding GSH-ACR, GSH-CA and GSH-MVK adducts via Michael addition reaction. These adducts are then further reduced to the corresponding alcohol forms by intracellular aldo-keto reductases in highly metastatic mouse melanoma (B16-BL6) cells and then excreted into the extracellular fluid. This time, we conducted a similar study using sheep erythrocytes and found analogous changes in the sheep erythrocytes after exposure to CSE as those with B16-BL6 cells. This indicates similarity of the detoxification pathways of the α,β-unsaturated carbonyl compounds in sheep blood cells and B16-BL6 cells. Also, we found that the GSH-MVK adduct was reduced by aldose reductase in a cell-free solution to generate its alcohol form, and its reduction reaction was completely suppressed by pretreatment with epalrestat, an aldose reductase inhibitor, a member of the aldo-keto reductase family. In the presence of sheep blood cells, however, reduction of the GSH-MVK adduct was partially inhibited by epalrestat. This revealed that some member of the aldo-keto reductase superfamily other than aldose reductase is involved in reduction of the GSH-MVK adduct in sheep blood. These results suggest that blood cells, mainly erythrocytes are involved in reducing the inhalation toxicity of cigarette smoke via an aldo-keto reductase pathway other than that of aldose reductase.

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Arsenite response in Coccomyxa sp. Carn explored by transcriptomic and non‐targeted metabolomic approaches

Cited by 20 publications

References 32 publications

Acidophilic green algal genome provides insights into adaptation to an acidic environment

Acidophilic green algal genome provides insights into adaptation to an acidic environment

Metagenomic and Metatranscriptomic Study of Microbial Metal Resistance in an Acidic Pit Lake

Detoxification Mechanism of α,β-Unsaturated Carbonyl Compounds in Cigarette Smoke Observed in Sheep Erythrocytes

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