Arsenite provides a selective signal that coordinates arsenate uptake and detoxification through the regulation of PHR1 stability in Arabidopsis

Navarro, Cristina; Mateo-Elizalde, Cristian; Mohan, Thotegowdanapalya C.; Sánchez-Bermejo, Eduardo; Urrutia, Óscar; Fernández-Muñiz, María Nieves; García-Mina, José M.; Muñoz, Riansares; Paz‐Ares, Javier; Castrillo, Gabriel; Leyva, Antonio

doi:10.1016/j.molp.2021.05.020

Cited by 24 publications

(15 citation statements)

References 102 publications

(134 reference statements)

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“…While the identification of the arsenic sensor in plants has remained elusive, we recently provide evidence toward the identification of the arsenic signaling molecule. Indeed, we have shown that As(III) is a key regulatory signal that regulates the arsenic response, even controlling the expression of the Pi/As(V) transporter in response to arsenic (Navarro et al, 2021). Notably, in line with the case in plants, in bacteria As(V) detoxification mechanisms are regulated by As(III), and likewise in yeast, As(III) is the arsenic signal as well (Xu et al, 1998;Di and Tamas, 2007;Kumar et al, 2015).…”

Section: Arsenic Perceptionsupporting

confidence: 62%

“…Therefore, it can be hypothesized that in response to arsenic, ASRF mediates the degradation of transcription factors involved in the activation of Pi/As(V) transporters, leading to the suppression of As(V) uptake. In addition, a second study showed that another F-box, named PHIF1, targets PHR1, the key activator of Pi/As(V) transporters, for ubiquitination and subsequent degradation in response to arsenic (Navarro et al, 2021).…”

Section: Post-transcriptional Regulationmentioning

confidence: 99%

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Arsenic perception and signaling: The yet unexplored world

Navarro

Navarro²,

Leyva³

2022

Front. Plant Sci.

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Arsenic is one of the most potent carcinogens in the biosphere, jeopardizing the health of millions of people due to its entrance into the human food chain through arsenic-contaminated waters and staple crops, particularly rice. Although the mechanisms of arsenic sensing are widely known in yeast and bacteria, scientific evidence concerning arsenic sensors or components of early arsenic signaling in plants is still in its infancy. However, in recent years, we have gained understanding of the mechanisms involved in arsenic uptake and detoxification in different plant species and started to get insights into arsenic perception and signaling, which allows us to glimpse the possibility to design effective strategies to prevent arsenic accumulation in edible crops or to increase plant arsenic extraction for phytoremediation purposes. In this context, it has been recently described a mechanism according to which arsenite, the reduced form of arsenic, regulates the arsenate/phosphate transporter, consistent with the idea that arsenite functions as a selective signal that coordinates arsenate uptake with detoxification mechanisms. Additionally, several transcriptional and post-translational regulators, miRNAs and phytohormones involved in arsenic signaling and tolerance have been identified. On the other hand, studies concerning the developmental programs triggered to adapt root architecture in order to cope with arsenic toxicity are just starting to be disclosed. In this review, we compile and analyze the latest advances toward understanding how plants perceive arsenic and coordinate its acquisition with detoxification mechanisms and root developmental programs.

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Section: Arsenic Perceptionsupporting

confidence: 62%

Section: Post-transcriptional Regulationmentioning

confidence: 99%

Arsenic perception and signaling: The yet unexplored world

Navarro

Navarro²,

Leyva³

2022

Front. Plant Sci.

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“…Increased expression of the GSH biosynthetic genes in siz1 mutant suggested a transcriptional regulation conferring SIZ1-mediated arsenite response. The transcription factor PHR1 is a master regulator for phosphate uptake and implicated in arsenic stress response (Navarro et al, 2021). PHR1 was also shown to be a SUMOylation target of SIZ1 (Miura et al, 2005).…”

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confidence: 99%

SUMO E3 ligase SIZ1 negatively regulates arsenite resistance via depressing GSH biosynthesis in Arabidopsis

et al. 2022

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Arsenic is a metalloid toxic to plants, animals and human beings. Small ubiquitin-like modifier (SUMO) conjugation is involved in many biological processes in plants. However, the role of SUMOylation in regulating plant arsenic response is still unclear. In this study, we found that dysfunction of SUMO E3 ligase SIZ1 improves arsenite resistance in Arabidopsis. Overexpression of the dominant-negative SUMO E2 variant resembled the arsenite-resistant phenotype of siz1 mutant, indicating that SUMOylation plays a negative role in plant arsenite detoxification. The siz1 mutant accumulated more glutathione (GSH) than the wild type under arsenite stress, and the arsenite-resistant phenotype of siz1 was depressed by inhibiting GSH biosynthesis. The transcript levels of the genes in the GSH biosynthetic pathway were increased in the siz1 mutant comparing with the wild type in response to arsenite treatment. Taken together, our findings revealed a novel function of SIZ1 in modulating plant arsenite response through regulating the GSH-dependent detoxification.

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“…oxides [4]. Also due to the similarities, arsenate (AsV) mimics phosphate (Pi) to share transporters, such as phosphate transporter 1 (PHT1) for uptake and Arabidopsis phosphate 1 (PHO1) for xylem loading and translocation [37]. As far as is known, relatively few enzymes utilize Pi (or AsV) directly as a substrate due to the irreversible nature of the majority of Piliberating processes [38].…”

Section: Discussionmentioning

confidence: 99%

“…The differential toxicity of the As chemical forms may come from the distinguished uptake mechanisms, in which the trivalent form (AsIII) is passively absorbed by plant membrane channels known as nodulin type 26 (NIP) [58], whereas the pentavalent forms (AsV) enter cells via PHT1-type membrane phosphate transporters [59]. For AsV uptake, it was related to an elegant mechanism through subcellular stimulus mediated by AsIII control AsV/Pi uptake depending upon the capacity of plants to detoxify AsV, minimizing plant accumulation and toxic effects [37,60]. Supporting this hypothesis, plants grown at the same concentrations of AsV and AsIII need a 4-fold increase in the pentavalent form to accumulate the same amount of total As in the tissues [18].…”

Section: Role Of Glutathione On As Tolerance: As(v) Vs As(iii) Compar...mentioning

confidence: 99%

Metallic elements in plants: bioaccumulation, phytoremediation potential and physiological responses

Coelho¹

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Pollution caused by metallic elements in the environment is becoming increasingly problematic worldwide. In the current work, several aspects of the metallic elements in plants were investigated, including the accumulation in species affected by an environmental disaster involving the disruption of a mining tailing dam; the physiological responses and phytoremediation potential of the aquatic macrophyte water lettuce (Pistia stratiotes) subjected to excess iron (Fe) and manganese (Mn), and combinations of Fe, Mn, and arsenic (As); and, finally, the in vivo monitoring of As-induced responses in Arabidopsis thaliana plants. We found a concerning enrichment of metal accumulation, especially for Fe and Mn, in the plant species evaluated in affected areas by mining tailings. Considering the spreading potential of contamination in aquatic environments, water lettuce plants, which are often used in phytoremediation studies, were tested for removal of excess Fe and Mn, along with the association with As. The plants displayed a great accumulation of Fe, mainly trapped in roots, whereas Mn was hyperaccumulated in shoots and roots. Accumulation of Mn was observed especially in the apoplast, avoiding major impairments of physiological processes. In presence of As, the plants displayed root loss as an acclimation response, followed by re-emission of the organs. Nonetheless, the specimens were able to maintain a high accumulation of the pollutants, supplied isolated or in association, demonstrating phytoremediation potential in multi- contaminated environments. Furthermore, the in vivo measurements using genetically-encoded biosensors showed intriguing stability of Mg-ATP 2− levels upon short-term arsenate (AsV) exposure. We also observed that the depletion of the GSH pool is the most likely cause of glutathione redox potential (E GSH ) oxidation. The findings presented here emphasize the importance of continuing to monitor metal-contaminated areas, as well as exposing alternatives for phytoremediation of aquatic environments and providing new insights into plant metabolism in response to pollutants. Keywords: Aquatic plants – Pollution effect. Aquatic plants – Heavy metal effects. Pistia stratiotes. Heavy metals. Aquatic plants – Metabolism.

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Arsenite provides a selective signal that coordinates arsenate uptake and detoxification through the regulation of PHR1 stability in Arabidopsis

Cited by 24 publications

References 102 publications

Arsenic perception and signaling: The yet unexplored world

Arsenic perception and signaling: The yet unexplored world

SUMO E3 ligase SIZ1 negatively regulates arsenite resistance via depressing GSH biosynthesis in Arabidopsis

Metallic elements in plants: bioaccumulation, phytoremediation potential and physiological responses

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