Arsenic Stress-Related F-Box (ASRF) gene regulates arsenic stress tolerance in Arabidopsis thaliana

Peña-Garcia, Yadira; Shinde, Suhas; Natarajan, Purushothaman; Lopez-Ortiz, Carlos; Balagurusamy, Nagamani; Chavez, Ana Cristina Delgado; Saminathan, Thangasamy; Nimmakayala, Padma; Reddy, Umesh K.

doi:10.1016/j.jhazmat.2020.124831

Cited by 15 publications

(7 citation statements)

References 88 publications

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“…The level of proline was associated with heavy metal stress, which was upregulated under the heavy metal stress . In addition, arsenic-related proline accumulation was reported in previous results, suggesting oxidative stress occurred. − Accordingly, the mitigation of As 3+ -induced proline accumulation by MT revealed the oxidative stress was alleviated in the presence of MT. N -Acetyl- l -ornithine also increased significantly under As 3+ stress compared with the control group, while it decreased significantly in the MT–As 3+ -treated group compared with that in As 3+ -treated group (Figure S6A).…”

Section: Resultsmentioning

confidence: 79%

Metallothionein Attenuated Arsenic-Induced Cytotoxicity: The Underlying Mechanism Reflected by Metabolomics and Lipidomics

Wang

et al. 2021

J. Agric. Food Chem.

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Arsenic ions (As3+) have been recognized as a hazard that threatens the health of humans. Metallothionein (MT) rich in cysteine may provide favorable binding sites for chelation of As3+. However, the influence of MT on As3+-induced toxicity and the underlying mechanism are poorly understood, especially at the metabolic level. Herein, the effects of MT on As3+-induced toxicity were evaluated. Cell viability analysis suggested that MT alleviated As3+-induced cytotoxicity. The metabolic response of PC12 cells to As3+ investigated by lipidomics and metabolomics indicated that the presence of As3+ disrupted phospholipids metabolism and induced cell membrane damage. Moreover, energy and amino acid metabolism were perturbed by As3+. The perturbation of As3+ on metabolism was further illustrated by the decrease of the mitochondrial membrane potential and the rise of cellular reactive oxygen species (ROS). On the contrary, MT rescued As3+-induced metabolic disorder and suppressed ROS accumulation. In addition, the binding process between As3+ and MT was characterized. The results proved that the As3+–MT complex was formed and chelated As3+-scavenged ROS, thus alleviating the toxic effects of As3+. These results revealed that MT would be a potential agent to reduce As3+-induced cytotoxicity.

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Section: Resultsmentioning

confidence: 79%

Metallothionein Attenuated Arsenic-Induced Cytotoxicity: The Underlying Mechanism Reflected by Metabolomics and Lipidomics

Wang

et al. 2021

J. Agric. Food Chem.

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“…In plants, aquaglyceroporins and phosphate transporters, respectively, facilitate the uptake of As V and As III from arseniccontaminated soils [21,22]. Once accumulated inside the plant tissues, arsenic can inhibit seed germination and seedling establishment, induce oxidative stress, inhibit photosynthesis, suppress growth, and reduce seed quality [23][24][25][26]. To protect themselves, plants deploy several coordinated defense processes, such as restriction of arsenic uptake and transportation, synthesis of arsenic chelating metabolites such as glutathione (GSH) and phytochelatins (PCs), stimulation of antioxidant defense, and vacuolar sequestration of arsenic to reduce arsenic-induced toxicity effects [25,27,28].…”

Section: Introductionmentioning

confidence: 99%

Strigolactones Modulate Cellular Antioxidant Defense Mechanisms to Mitigate Arsenate Toxicity in Rice Shoots

Mostofa

Rahman

et al. 2021

Antioxidants

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Metalloid contamination, such as arsenic poisoning, poses a significant environmental problem, reducing plant productivity and putting human health at risk. Phytohormones are known to regulate arsenic stress; however, the function of strigolactones (SLs) in arsenic stress tolerance in rice is rarely investigated. Here, we investigated shoot responses of wild-type (WT) and SL-deficient d10 and d17 rice mutants under arsenate stress to elucidate SLs’ roles in rice adaptation to arsenic. Under arsenate stress, the d10 and d17 mutants displayed severe growth abnormalities, including phenotypic aberrations, chlorosis and biomass loss, relative to WT. Arsenate stress activated the SL-biosynthetic pathway by enhancing the expression of SL-biosynthetic genes D10 and D17 in WT shoots. No differences in arsenic levels between WT and SL-biosynthetic mutants were found from Inductively Coupled Plasma-Mass Spectrometry analysis, demonstrating that the greater growth defects of mutant plants did not result from accumulated arsenic in shoots. The d10 and d17 plants had higher levels of reactive oxygen species, water loss, electrolyte leakage and membrane damage but lower activities of superoxide dismutase, ascorbate peroxidase, glutathione peroxidase and glutathione S-transferase than did the WT, implying that arsenate caused substantial oxidative stress in the SL mutants. Furthermore, WT plants had higher glutathione (GSH) contents and transcript levels of OsGSH1, OsGSH2, OsPCS1 and OsABCC1 in their shoots, indicating an upregulation of GSH-assisted arsenic sequestration into vacuoles. We conclude that arsenate stress activated SL biosynthesis, which led to enhanced arsenate tolerance through the stimulation of cellular antioxidant defense systems and vacuolar sequestration of arsenic, suggesting a novel role for SLs in rice adaptation to arsenic stress. Our findings have significant implications in the development of arsenic-resistant rice varieties for safe and sustainable rice production in arsenic-polluted soils.

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“…Furthermore, F-box proteins mediating protein ubiquitination in the SCF complex are essential for abiotic stress responses [ 100 ] and have also been reported to control Pi homeostasis [ 11 ]. An Arsenic Stress-Related F-box gene ( ASRF ) has been reported earlier by our group as a negative regulator of arsenic tolerance in Arabidopsis [ 103 ]. ASRF might function in a feedback loop to fine-tune the Pi equilibrium related to the phosphate starvation response caused by As-stress exposure.…”

Section: Reduction and Sequestration Of Arsenic In Plantsmentioning

confidence: 99%

From genes to ecosystems: Decoding plant tolerance mechanisms to arsenic stress

Gracia-Rodriguez,

Lopez-Ortiz,

Flores-Iga

et al. 2024

Heliyon

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Arsenic Stress-Related F-Box (ASRF) gene regulates arsenic stress tolerance in Arabidopsis thaliana

Cited by 15 publications

References 88 publications

Metallothionein Attenuated Arsenic-Induced Cytotoxicity: The Underlying Mechanism Reflected by Metabolomics and Lipidomics

Metallothionein Attenuated Arsenic-Induced Cytotoxicity: The Underlying Mechanism Reflected by Metabolomics and Lipidomics

Strigolactones Modulate Cellular Antioxidant Defense Mechanisms to Mitigate Arsenate Toxicity in Rice Shoots

From genes to ecosystems: Decoding plant tolerance mechanisms to arsenic stress

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