Arsenic species and selected metals in human urine: validation of HPLC/ICPMS and ICPMS procedures for a long-term population-based epidemiological study

Abstract

Exposure to high inorganic arsenic concentrations in drinking water has been related to detrimental health effects, including cancers and possibly cardiovascular disease, in many epidemiological studies. Recent studies suggest that arsenic might elicit some of its toxic effects also at lower concentrations. The Strong Heart Study, a large epidemiological study of cardiovascular disease in American Indian communities, collected urine samples and performed medical examinations on 4,549 participants over a 10-year period beginning in 1989. We used anion-exchange HPLC/ICPMS to determine concentrations of arsenic species (methylarsonate, dimethylarsinate and arsenate) in 5,095 urine samples from the Strong Heart Study. We repeated the chromatography on a portion of the urine sample that had been oxidised, by addition of H2O2, to provide additional information on the presence of As(III) species and thio-arsenicals, and by difference, of arsenobetaine and other non-retained cations. Total concentrations for As, Cd, Mo, Pb, Sb, Se, U, W, and Zn were also determined in the urine samples by ICPMS. The dataset will be used to evaluate the relationships between the concentrations of urinary arsenic species and selected metals with various cardiometabolic health endpoints. We present and discuss the analytical protocol put in place to produce this large and valuable dataset.

Multi-Element Analysis of Some Toxic Metals in Urine using Quadrupole ICP-MS

“…The biggest variations, either in relation to the determinations performed in the same day, as in those performed in different periods, were observed for 78 Se. These variations corroborate with results from other studies in the literature 37,45 .…”

“…In is used for the intermediate masses and 185 Re for the higher ones (from 150 to 240 amu) [36][37][38][39] . The choice of the internal standards for the present study was also based in the preliminary results obtained for the accuracy and recuperation parameters.…”

Estimation of Inorganic Arsenic Exposure in Populations With Frequent Seafood Intake: Evidence From MESA and NHANES

“…The percentages of participants with urinary concentrations below the limit of detection was 3.9%, 0%, 14.2%, and 45.8% for arsenobetaine, DMA, MMA, and arsenate, respectively. The interassay coefficients of variation for iAs, MMA, DMA, and arsenobetaine were 6.0%, 6.5%, 5.9%, and 6.5%, respectively (34).…”

“…using anion-exchange high-performance liquid chromatography (Agilent 1100, Agilent Technologies, Waldbronn, Germany) coupled with inductively coupled plasma mass spectrometry (Agilent 7700x ICPMS, Agilent Technologies) following an established protocol (34). Arsenite was oxidized to arsenate with hydrogen peroxide to assess overall iAs.…”

Analysis of Main Arsenic Species in Canned Fish Marketed in the Czech Republic and Austria

“…This problem is frequently solved by analysis of the sum of As(III) and As(V) instead of individual species. Therefore, in some procedures, As(III) is converted to As(V) by oxidation with hydrogen peroxide prior to chromatographic analysis (see [17,18] for fish tissue analysis, [19] for analysis of urine or [20] for analysis of blood). The other species of arsenic MA, DMA, and As(V) are separated with very good resolution.…”