Arsenic inhibits induction of cytochrome P450 1A1 by 2,3,7,8-tetrachlorodibenzo-p-dioxin in human hepatoma cells

Chao, How‐Ran; Tsou, Tsui‐Chun; Li, Lih-Ann; Tsai, Feng-Yuan; Wang, Ya-Fen; Tsai, Cheng‐Hsien; Chang, Eddy Essen; Miao, Zhi-Feng; Wu, Chi-Hwa; Lee, Wen-Jhy

doi:10.1016/j.jhazmat.2006.03.053

Cited by 39 publications

(22 citation statements)

References 26 publications

(32 reference statements)

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“…With a stably transfected PPRE-driven luciferase gene, the cell line was designed to identify those potential environmental obesogens with PPAR activity. Previous studies have reported that some parameters for cell culture, such as FBS in culture medium (Laier et al 2003) and exposure times (Chao et al 2006), may affect luciferase reporter activity. Also, Phenol Red, widely used as a pH indicator in culture medium, is a weak estrogen (Berthois et al 1986) and thus may interfere with the action of PPARa on adipogenesis (Jeong and Yoon 2007).…”

Section: Resultsmentioning

confidence: 99%

“…Transfection was performed as previously described with modifications (Chao et al 2006). Briefly, Huh7 cells seeded in 60 mm dishes were cotransfected with a reporter vector, PPRE93-tk-Luc (Kliewer et al 1992), and a selection vector, pSUPER-EGFP-neo, according to the Lipofectamine reagent protocol (Life Technologies, Grand Island, NY, USA).…”

Section: Establishment Of Recombinant Huh7-ppre-luc Cells For Ppre-drmentioning

confidence: 99%

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A recombinant peroxisome proliferator response element-driven luciferase assay for evaluation of potential environmental obesogens

Wang

Chao

et al. 2010

Biotechnol Lett

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A recombinant Huh7-PPRE-Luc cell line for analyzing the peroxisome proliferator response element (PPRE)-driven luciferase activity was established. The cells exhibited a good dose-response induction in PPRE-driven luciferase activity by three subtypes of peroxisome proliferator-activated receptor (PPAR) agonists as well as by a retinoid X receptor agonist, 9-cis-retinoic acid. Among five environmental chemicals tested, benzyl butyl phthalate and bisphenol induced PPRE-driven luciferase activation in Huh7-PPRE-Luc cells and caused adipogenic differentiation of 3T3-L1 cells. This recombinant Huh7-PPRE-Luc cell line would be useful for screening potential environmental obesogens with PPAR activity.

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Section: Resultsmentioning

confidence: 99%

Section: Establishment Of Recombinant Huh7-ppre-luc Cells For Ppre-drmentioning

confidence: 99%

A recombinant peroxisome proliferator response element-driven luciferase assay for evaluation of potential environmental obesogens

Wang

Chao

et al. 2010

Biotechnol Lett

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“…[3]. Luciferase activity was determined in the programmed microplate luminometer MicroLumatPlus LB96V (EG&G Berthold, Germany).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

“…Our previous studies have revealed that arsenic [3] as well as arecoline [4] inhibits induction of CYP1A1 by TCDD. In arsenic study, as expected, CYP1A1 activities by ethoxyresorufin-Odeethylase (EROD) assay were highly parallel with those by DRE-CALUX bioassay [3]. Combined with our previous arecoline study [4], here we demonstrate that arecoline inhibits the TCDD-induced CYP1A1 activations, however with enhanced TCDD-induced DRE-CALUX activations.…”

Section: Introductionmentioning

confidence: 97%

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Differential effect of arecoline on the endogenous dioxin-responsive cytochrome P450 1A1 and on a stably transfected dioxin-responsive element-driven reporter in human hepatoma cells

Chao

Wang

Chen

et al. 2007

Journal of Hazardous Materials

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In Vitro Liver Systems to Study Induction/Inhibition: Prediction of In Vivo Metabolism and Drug–Drug Interactions

Kono

Iwaki

2012

Encyclopedia of Drug Metabolism and Interactions

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This chapter was the review focused on the evaluation systems for metabolic stability, CYP inhibition and induction not only in the selection process of development candidates including lead optimizations in drug discovery but also to support a design of clinical studies in drug development. In the former process, the advanced in vitro systems have been developed, associating with an inevitable paradigm shift from the throughput to the quality. The similar trend has been gradually transmitted to the prediction of clearance and DDI. The predictions appeared to be in the movement from the basic models to the more mechanistic models including PBPK.

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Arsenic inhibits induction of cytochrome P450 1A1 by 2,3,7,8-tetrachlorodibenzo-p-dioxin in human hepatoma cells

Cited by 39 publications

References 26 publications

A recombinant peroxisome proliferator response element-driven luciferase assay for evaluation of potential environmental obesogens

A recombinant peroxisome proliferator response element-driven luciferase assay for evaluation of potential environmental obesogens

Differential effect of arecoline on the endogenous dioxin-responsive cytochrome P450 1A1 and on a stably transfected dioxin-responsive element-driven reporter in human hepatoma cells

In Vitro Liver Systems to Study Induction/Inhibition: Prediction of In Vivo Metabolism and Drug–Drug Interactions

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