Arsenic-induced autophagic alterations and mitochondrial impairments in HPG-S axis of mature male mice offspring (F1-generation): A persistent toxicity study

Ommati, Mohammad Mehdi; Manthari, Ram Kumar; Tikka, Chiranjeevi; Niu, Ruiyan; Sun, Zilong; Sabouri, Samira; Zamiri, Mohammad Javad; Ahmadi, Hasan Nategh; Ghaffari, Hasti; Heidari, Reza; Wang, Jundong

doi:10.1016/j.toxlet.2020.02.013

Cited by 47 publications

(41 citation statements)

References 81 publications

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“…The hypo-osmotic swelling (HOS) test, as a valid index of membrane integrity, was performed by mixing 10 µL of sperm suspension with 50 µL of hypo-osmotic solution (50-mOsm NaCl) for 10 min at 37 • C and calculating the percentages of spermatozoa (in at last 200 sperm per slide) with a swollen "bubble" around the curled flagellum, using light microscopy (1,000 × magnification) (31,56). Sperm abnormality and viability were measured in duplicate (200 sperm per sample) after eosin-nigrosin staining (20,31). Spermatozoa with protoplasmic droplets, ab-axial tails, malformed heads, double tails, coiled tails, bent tails, and without tail or head were recorded as abnormal under a phase-contrast microscope (Olympus BX41; Olympus Optical Co. Ltd, Japan) (28,57).…”

Section: Sperm Quality Evaluationmentioning

confidence: 99%

“…It is well-known that there is a close relation between reproduction and developmental toxicity. Reprotoxicity is one of the crucial parts of chemicals risk assessments by evaluating the internal organs and endocrine system (i.e., hypothalamuspituitary-gonad-sperm axis, HPG-S) (20,21). Additionally, there is a need to clarify the reproductive and developmental health effects of CNTs (18).…”

Section: Introductionmentioning

confidence: 99%

“…Oxidative stress (OS), mitochondrial impairment, and their subsequent complications seem to play crucial roles in the xenobiotics-induced reprotoxicity and consequent infertility (20,21,(24)(25)(26)(27)(28)(29)(30). High sensitivity to OS, excessive reactive oxygen species (ROS) levels, and thiobarbituric acid reactive substances (TBARS) content could dramatically affect the functions of the reproductive organs (20,21,(26)(27)(28)(29)(30)(31)(32)(33)(34). For instance, oxidative injury and DNA damage studies indicated that high doses of MWCNT could cause DNA damage in murine sperm (35).…”

Section: Introductionmentioning

confidence: 99%

“…Cellular energy metabolism is pivotal in sperm forward motility (24,25,51), and there is ample evidence showing that xenobiotics could alter mitochondrial functionality and induce severe OS in different reproductive tissues (20,21,(27)(28)(29)(30). In this regard, Xu et al (52) reported that MWCNT caused severe damages to mitochondrial DNA in spermatocytes.…”

Section: Introductionmentioning

confidence: 99%

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Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

et al. 2020

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Carbon-based nanomaterials possess a remarkably high potential for biomedical applications due to their physical properties; however, their detrimental effects on reproduction are also concerned. Several reports indicate the toxicity of carbon nanotubes (CNT); nevertheless, their impact on intracellular organelles in the male reproductive organs has not been fully elucidated. Herein, we report on the reprotoxicity of single-walled (SWCNT) and multi-walled carbon nanotubes (MWCN) on several intracellular events and histological criteria in pubertal male BALB/c mice orally treated with 0, 10, and 50 mg/kg/day doses for 5 weeks. Biomarkers of oxidative stress and mitochondrial functionality, histopathological alterations, and epididymal sperm characteristics were determined. Oral administration of CNTs at 10 and 50 mg/kg evoked a significant decrement in weight coefficient, sperm viability and motility, hypo-osmotic swelling (HOS) test, sperm count, mitochondrial dehydrogenase activity, ATP content, total antioxidant capacity, and GSH/GSSH ratio in the testis and epididymal spermatozoa. On the other hand, percent abnormal sperm, testicular and sperm TBARS contents, protein carbonylation, ROS formation, oxidized glutathione level, and sperm mitochondrial depolarization were considerably increased. Significant histopathological and stereological alterations in the testis occurred in the groups challenged with CNTs. The current findings indicated that oxidative stress and mitochondrial impairment might substantially impact CNTs-induced reproductive system injury and sperm toxicity. The results can also be used to establish environmental standards for CNT consumption by mammals, produce new chemicals for controlling the rodent populations, and develop therapeutic approaches against CNTs-associated reproductive anomalies in the males exposed daily to these nanoparticles.

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Section: Sperm Quality Evaluationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

et al. 2020

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“…The mitochondrial capacity to capture the cationic fluorescent dye, rhodamine 123, was used to estimate mitochondrial depolarization [ 47 , 62 , 70 - 74 ]. Rhodamine 123 accumulates in the mitochondrial matrix by facilitated diffusion.…”

Section: Methodsmentioning

confidence: 99%

Taurine mitigates cirrhosis-associated heart injury through mitochondrial-dependent and antioxidative mechanisms

Mousavi¹,

Niknahad²,

Mostofi³

et al. 2020

ceh

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Drug‐induced organ injury in coronavirus disease 2019 pharmacotherapy: Mechanisms and challenges in differential diagnosis and potential protective strategies

Ommati

Mobasheri

Heidari

2021

J Biochem & Molecular Tox

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The world is currently facing an unprecedented pandemic caused by a newly recognized and highly pathogenic coronavirus disease 2019 (COVID‐19; induced by SARS‐CoV‐2 virus), which is a severe and ongoing threat to global public health. Since COVID‐19 was officially declared a pandemic by the World Health Organization in March 2020, several drug regimens have rapidly undergone clinical trials for the management of COVID‐19. However, one of the major issues is drug‐induced organ injury, which is a prominent clinical challenge. Unfortunately, most drugs used against COVID‐19 are associated with adverse effects in different organs, such as the kidney, heart, and liver. These side effects are dangerous and, in some cases, they can be lethal. More importantly, organ injury is also a clinical manifestation of COVID‐19 infection. These adverse reactions are increasingly recognized as outcomes of COVID‐19 infection. Therefore, the differential diagnosis of drug‐induced adverse effects from COVID‐19‐induced organ injury is a clinical complication. This review highlights the importance of drug‐induced organ injury, its known mechanisms, and the potential therapeutic strategies in COVID‐19 pharmacotherapy. We review the potential strategies for the differential diagnosis of drug‐induced organ injury. This information can facilitate the development of therapeutic strategies, not only against COVID‐19 but also for future outbreaks of other emerging infectious diseases.

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Arsenic-induced autophagic alterations and mitochondrial impairments in HPG-S axis of mature male mice offspring (F1-generation): A persistent toxicity study

Cited by 47 publications

References 81 publications

Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

Taurine mitigates cirrhosis-associated heart injury through mitochondrial-dependent and antioxidative mechanisms

Drug‐induced organ injury in coronavirus disease 2019 pharmacotherapy: Mechanisms and challenges in differential diagnosis and potential protective strategies

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