Arsenic induced apoptosis in malignant melanoma cells is enhanced by menadione through ROS generation, p38 signaling and p53 activation

Chowdhury, Rajdeep; Chowdhury, Suchandra; Roychoudhury, Paromita; Mandal, Chitra; Chaudhuri, Keya

doi:10.1007/s10495-008-0284-8

Cited by 75 publications

(54 citation statements)

References 50 publications

(72 reference statements)

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“…85 Recent studies have suggested that p53 plays a critical role in the cellular response to DNA damage and apoptosis induced by ROS. 86,87 Our Western blotting data ( Figure 10) suggest that both B-AgNPs and F-AgNPs activated p53, which mediated apoptosis. This result is consistent with previous studies by Lu et al and Mroz et al, which showed that nanoparticles and ROS can induce DNA damage, activate p53, and mimic irradiation-related carcinogenesis pathways.…”

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confidence: 86%

Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Gurunathan¹,

Park²,

Han³

et al. 2015

IJN

272

151

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Background Recently, the use of nanotechnology has been expanding very rapidly in diverse areas of research, such as consumer products, energy, materials, and medicine. This is especially true in the area of nanomedicine, due to physicochemical properties, such as mechanical, chemical, magnetic, optical, and electrical properties, compared with bulk materials. The first goal of this study was to produce silver nanoparticles (AgNPs) using two different biological resources as reducing agents, Bacillus tequilensis and Calocybe indica. The second goal was to investigate the apoptotic potential of the as-prepared AgNPs in breast cancer cells. The final goal was to investigate the role of p53 in the cellular response elicited by AgNPs. Methods The synthesis and characterization of AgNPs were assessed by various analytical techniques, including ultraviolet-visible (UV-vis) spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), and transmission electron microscopy (TEM). The apoptotic efficiency of AgNPs was confirmed using a series of assays, including cell viability, leakage of lactate dehydrogenase (LDH), production of reactive oxygen species (ROS), DNA fragmentation, mitochondrial membrane potential, and Western blot. Results The absorption spectrum of the yellow AgNPs showed the presence of nanoparticles. XRD and FTIR spectroscopy results confirmed the crystal structure and biomolecules involved in the synthesis of AgNPs. The AgNPs derived from bacteria and fungi showed distinguishable shapes, with an average size of 20 nm. Cell viability assays suggested a dose-dependent toxic effect of AgNPs, which was confirmed by leakage of LDH, activation of ROS, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive cells in MDA-MB-231 breast cancer cells. Western blot analyses revealed that AgNPs induce cellular apoptosis via activation of p53, p-Erk1/2, and caspase-3 signaling, and downregulation of Bcl-2. Cells pretreated with pifithrin-alpha were protected from p53-mediated AgNPs-induced toxicity. Conclusion We have demonstrated a simple approach for the synthesis of AgNPs using the novel strains B. tequilensis and C. indica , as well as their mechanism of cell death in a p53-dependent manner in MDA-MB-231 human breast cancer cells. The present findings could provide insight for the future development of a suitable anticancer drug, which may lead to the development of novel nanotherapeutic molecules for the treatment of cancers.

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confidence: 86%

Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Gurunathan¹,

Park²,

Han³

et al. 2015

IJN

272

151

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“…Under normal circumstances, its production and resolution are in dynamic balance, and the redox state of the intracellular environment maintains a stable state. A great deal of research indicated that many antitumor drugs are closely related to the ROS initiation within tumor cells [19][20][21]. Such as antitumor drug arsenic trioxide (As 2 O 3 ), polysaccharides, quinone anticancer drugs, and so on, they can produce a lot of ROS inducing tumor cell apoptosis [22].…”

Section: Discussionmentioning

confidence: 99%

Flavonoids from tartary buckwheat induce G<sub>2</sub>/M cell cycle arrest and apoptosis in human hepatoma HepG2 cells

Duan

Jia

et al. 2014

ABBS

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The cytotoxicity and antioxidant activity on human hepatoma cell line HepG2 of three flavonoids homogenous compounds from tartary buckwheat seeds and bran, namely quercetin, isoquercetin, and rutin, were investigated. The total antioxidant competency detection results indicated that the antioxidant capacity of quercetin was the strongest in a biological response system. A [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay showed that quercetin exhibited the strongest cytotoxic effects against the HepG2 cell line. Flow cytometric analysis indicated that quercetin significantly increased the production of reactive oxygen species, and led to the G 2 /M phase arrest accompanied by an increase of apoptotic cell death after 48 h of incubation. Quercetin-induced cell apoptosis was shown to involve p53 and p21 up-regulation, Cyclin D1, Cdk2, and Cdk7 down-regulation. These results suggested that the induction of G 2 /M arrest, apoptosis, and cell death by quercetin may associate with increased expression of p53 and p21, decrease of Cyclin D1, Cdk2, and Cdk7 levels, and generation of reactive oxygen species in cells. This study will help to better understand and fully utilize medicinal resources of plant flavonoids.

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“…These findings revealed that cell viability in response to arsenite is regulated by stimulation of GSH synthesis in GCs, and that BSO abolished arsenic-mediated progesterone production may be due to cell apoptosis. In many cases, arsenite increases ROS generation and facilitates the major apoptotic signaling events (Chowdhury et al, 2010). Ghibelli et al (1998) reported that GSH played an anti-apoptotic role in apoptogenic treatment cells.…”

Section: Journal Of Cellular Physiologymentioning

confidence: 99%

Arsenic induced progesterone production in a caspase‐3‐dependent manner and changed redox status in preovulatory granulosa cells

Yuan

Yao

et al. 2011

Journal Cellular Physiology

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Arsenic contamination is a principal environmental health threat throughout the world. However, little is known about the effect of arsenic on steroidogenesis in granulosa cells (GCs). We found that the treatment of preovulatory GCs with arsenite stimulated progesterone production. A significant increase in serum level of progesterone was observed in female Sprague-Dawley rats following arsenite treatment at a dose of 10 mg/L/rat/day for 7 days. Further experiments demonstrated that arsenite treatment did not change the level of intracellular cyclic AMP (cAMP) or phosphorylated ERK1/2 in preovulatory GCs; however, progesterone production was significantly decreased when cAMP-dependent protein kinase (PKA) or ERK1/2 pathway was inhibited. This implied that the effect of arsenite on progesterone production may require cAMP/PKA and ERK1/2 signaling but not depend on them. Furthermore, we found that arsenite decreased intracellular reactive oxygen species (ROS) but increased the antioxidant glutathione (GSH) levels and mitochondrial membrane potential (ΔΨm) in parallel to the changes in progesterone production. Progesterone antagonist blocked the arsenic-stimulated increase of GSH levels. Arsenite treatment induced caspase-3 activation, although no apoptosis was observed. Inhibition of caspase-3 activity significantly decreased progesterone production stimulated by arsenite or follicle-stimulating hormone (FSH). GSH depletion with buthionine sulfoximine led to cell apoptosis in response to arsenite treatment. Collectively, this study demonstrated for the first time that arsenite stimulates progesterone production through cleaved/active caspase-3-dependent pathway, and the increase of GSH level promoted by progesterone production may protect GCs against apoptosis and maintain the steroidogenesis of GCs in response to arsenite treatment.

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Arsenic induced apoptosis in malignant melanoma cells is enhanced by menadione through ROS generation, p38 signaling and p53 activation

Abstract: This study thus provides an insight into innovative mechanisms of melanoma sensitization, a proper cure against which is still elusive. Taken together, our data also provides the first evidence of arsenic activity accentuation by menadione through modulation of specific signaling-pathways.

Cited by 75 publications

References 50 publications

Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Flavonoids from tartary buckwheat induce G<sub>2</sub>/M cell cycle arrest and apoptosis in human hepatoma HepG2 cells

Arsenic induced progesterone production in a caspase‐3‐dependent manner and changed redox status in preovulatory granulosa cells

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Arsenic induced apoptosis in malignant melanoma cells is enhanced by menadione through ROS generation, p38 signaling and p53 activation

Cited by 75 publications

References 50 publications

Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in&nbsp;MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in&nbsp;MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Flavonoids from tartary buckwheat induce G&lt;sub&gt;2&lt;/sub&gt;/M cell cycle arrest and apoptosis in human hepatoma HepG2 cells

Arsenic induced progesterone production in a caspase‐3‐dependent manner and changed redox status in preovulatory granulosa cells

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Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

Flavonoids from tartary buckwheat induce G<sub>2</sub>/M cell cycle arrest and apoptosis in human hepatoma HepG2 cells