Arsenic exposure alters hepatic arsenic species composition and stress-mediated gene expression in the common killifish (Fundulus heteroclitus)

Bears, Heather; Richards, Jeff G.; Schulte, Patricia M.

doi:10.1016/j.aquatox.2005.12.008

Cited by 88 publications

(46 citation statements)

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“…Primers for ERRa, ERRba, ERRbb, ERRgb, and b-actin (Table 1) were designed with one primer spanning a predicted exon-exon junction to avoid amplification of genomic DNA. Primers for EF-1 (Bears et al 2006) and vitellogenin (Garcio-Reyero et al 2004) were taken from published studies. cDNA was synthesized from 2 mg total RNA using random hexamers and the Omniscript cDNA Synthesis Kit (Qiagen).…”

Section: Nomenclaturementioning

confidence: 99%

“…Expression data were quantified based on threshold cycle (C t ) values and the -2 DDC t method (Livak & Schittgen, 2001). b-Actin expression was highly variable among tissues (data not shown), so for each ERR gene, C t values were normalized to EF-1 (Bears et al 2006). Relative mRNA expression for each gene was calculated as the fold change compared with the tissue with the lowest C t (i.e.…”

Section: Nomenclaturementioning

confidence: 99%

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Estrogen receptor-related receptors in the killifish Fundulus heteroclitus: diversity, expression, and estrogen responsiveness

Tarrant¹,

Greytak²,

Callard³

et al. 2006

Journal of Molecular Endocrinology

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The estrogen receptor-related receptors (ERRs) are a group of nuclear receptors that were originally identified on the basis of sequence similarity to the estrogen receptors. The three mammalian ERR genes have been implicated in diverse physiological processes ranging from placental development to maintenance of bone density, but the diversity, function, and regulation of ERRs in non-mammalian species are not well understood. In this study, we report the cloning of four ERR cDNAs from the Atlantic killifish, Fundulus heteroclitus, along with adult tissue expression and estrogen responsiveness. Phylogenetic analysis indicates that F. heteroclitus (Fh)ERRa is an ortholog of the single ERRa identified in mammals, pufferfish, and zebrafish. FhERRba and FhERRbb are co-orthologs of the mammalian ERRb. Phylogenetic placement of the fourth killifish ERR gene, tentatively identified as FhERRgb, is less clear. The four ERRs showed distinct, partially overlapping mRNA expression patterns in adult tissues. FhERRa was broadly expressed. FhERRba was expressed at apparently low levels in eye, brain, and ovary. FhERRbb was expressed more broadly in liver, gonad, eye, brain, and kidney. FhERRgb was expressed in multiple tissues including gill, heart, kidney, and eye. Distinct expression patterns of FhERRba and FhERRbb are consistent with subfunctionalization of the ERRb paralogs. Induction of ERRa mRNA by exogenous estrogen exposure has been reported in some mammalian tissues. In adult male killifish, ERR expression did not significantly change following estradiol injection, but showed a trend toward a slight induction (three-to five-fold) of ERRa expression in heart. In a second, more targeted experiment, expression of ERRa in adult female killifish was downregulated 2$5-fold in the heart following estradiol injection. In summary, our results indicate that killifish contain additional ERR genes relative to mammals, including ERRb paralogs. In addition, regulation of ERRa expression in killifish apparently differs from regulation in mammals. Together, these features may facilitate determination of both conserved and specialized ERR gene functions.

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Section: Nomenclaturementioning

confidence: 99%

Section: Nomenclaturementioning

confidence: 99%

Estrogen receptor-related receptors in the killifish Fundulus heteroclitus: diversity, expression, and estrogen responsiveness

Tarrant¹,

Greytak²,

Callard³

et al. 2006

Journal of Molecular Endocrinology

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“…Fish can be considered as indicator organisms of environmental pollution (Metcalfe-Smith et al 2003, Bhatnagar and Regar 2005, Bhatnagar et al 2007. This is because they are constantly exposed to toxic elements dissolved in water, through gill breathing and epidermis contact (Gernhöfer et al 2001, Bears et al 2006.…”

Section: Introductionmentioning

confidence: 99%

“…The mechanisms of As incorporated into fish are unclear; there are only a few studies about the detoxification mechanism (Wrobel et al 2002, Bears et al 2006). Regarding F -, studies analyzing its biotransference from water to fish tissues are also scarce (Haque et al 2012, Cao et al 2013, Puntoriero et al 2014b.…”

Section: Introductionmentioning

confidence: 99%

HISTOPATHOLOGICAL CHANGES IN LIVER AND GILLS OF Odontesthes bonariensis INHABITING A LAKE WITH HIGH CONCENTRATIONS OF ARSENIC AND FLUORIDE (CHASICÓ LAKE, BUENOS AIRES PROVINCE)

Puntoriero¹,

Cirelli²,

Volpedo³

2018

Rev. Int. Contam. Ambie.

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Water bodies in the Chaco-Pampean plain contain arsenic (As) and fluoride (F -) occurring naturally. Chasicó Lake, located in the southwest of Buenos Aires Province, has high concentrations of both elements (As, 0.05-0.41 mg/L; F -, 6.74-8.54 mg/L). Fish can be considered as indicators of environmental pollution. The aim of this study was to analyze whether the presence of high concentrations of As and F -found in the liver and gills of Odonthestes bonariensis could be associated to histological changes found in these tissues. Fish were sampled from different locations of the lake. Tissue samples (liver and gills) were subdivided and subjected to an acid digestion to determine As and to an alkaline digestion to determine F -. Morphological changes were assessed using routine histological techniques. As concentrations in the liver were higher than in the gills, while the gills showed higher F -concentrations. The histological examination of the liver and gills showed alterations that could correspond to the effects of the high As and F -concentrations found in these tissues. The toxicity of these elements is due to oxidative stress. Silverside is one commercially relevant fish in Buenos Aires and a key species in the food chain in the region. Therefore, further histopathological studies are relevant to guarantee this fish's health and safety for human consumption.Palabras clave: histopatología, elementos traza, pejerrey, Argentina RESUMEN Los cuerpos de agua de la llanura Chaco-Pampeana contienen arsénico (As) y fluoruro (F -) cuyo origen es natural. El Lago Chasicó, ubicado en el sudoeste bonaerense, presenta elevadas concentraciones de ambos elementos (As: 0.05-0.41 mg/L; F -: 6.74-8.54 mg/L). Los peces pueden considerarse como indicadores de contaminación ambiental. El objetivo de este estudio fue analizar si la presencia de altas concentraciones de As y F -halladas en hígado y branquias de Odonthestes bonariensis pudiera estar asociada con los cambios histológicos encontrados en estos tejidos. Los peces fueron colectados en diferentes localidades del lago. Se subdividieron muestras de tejidos (hígado y branquias) y se sometieron a una digestión ácida para determinar As y a una digestión Rev. Int. Contam. Ambie. 34 (1) 69-77, 2018 DOI: 10.20937/RICA.2018 M.L. Puntoriero et al. 70alcalina para determinar F -. Los cambios morfológicos se determinaron mediante técnicas histológicas de rutina. En hígado se hallaron concentraciones de As más altas que en branquias, mientras que las branquias mostraron concentraciones de F -más elevadas. El examen histológico del hígado y las branquias mostró alteraciones que podrían corresponder a los efectos de las altas concentraciones de As y F -halladas en esos tejidos. La toxicidad de estos elementos se debe al estrés oxidativo. El pejerrey es uno de los peces comerciales más importantes de Buenos Aires y una de las especies clave de la cadena alimenticia en la región, por lo que resultan relevantes estudios histopatológicos adicionales para asegurar la salud de los ...

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“…Most of the As compounds are used in manufacture of agricultural products such as insecticides, herbicides, fungicides, algaecides, wood preservatives, and growth stimulants for plants and animals. The atmospheric emissions from smelters, coal-fired power plants and arsenical herbicide sprays; water contaminated by mine tailings, smelter wastes and natural mineralization; and diet, especially from consumption of marine biota, all cause As toxicity [37].In freshwater fish, As can be present in two different oxidation states, as arseno-sugars and arseno-lipids, these two differ in their toxicity which is supposed to be responsible for the pathophysiology of arsenic [38]. Arsenobetaine and arsenocholine are non-toxic organic forms of arsenic present in fish.…”

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confidence: 99%

Environmental Health Monitoring: A Pragmatic Approach

Vincent¹

2014

Int J Waste Resources

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Eco benign environment, wherein human race survives and evolves, comprises the interaction of biotic and abiotic systems. Maintaining such environment in view of drastically changing human practices due to increased industrialization, urbanization and modernization is an urgent necessity for the global human community. The responsibility to protect the environment as well as to make it suitable for sustaining human life with well-being propels research-based implementation of best industrial and environmental practices. The nature of strategies and research designed for arriving eco-benign solutions to the challenges of environmental protection widely varies with different fields of industry. It is important to note that the intervention of environmental friendly strategies for making hazard and disease free environment is inevitable in all spheres of life, and the research on green solutions envisaged for management of environmental resources is the need of hour. Hence, in this review, we highlight three socially important environmental research areas such as impact of toxicity of metal pollutants in aquatic biological systems with special reference to fish as a bioindicator, mosquito control by bioinsecticides, and management of disease and disaster management by Geographical Information Systems (GIS) that are of our areas of contribution. Though these areas are viewed as different domains, they share an unique feature among themselves i.e. the crisis in water management affecting environment and health. The review also attempts to discuss about the possible biointervention solutions to address the challenges in water management in terms of pollution.Keywords: Metal toxicity; Aquatic health; Biomonitoring; Metallothionein; Bioinsecticides; GIS; Drainage Changing Quality of Aquatic LifeQuality of life in aquatic system is determined by the characteristics of biotic resources and their interactions that are complex and dynamic. Naturally, there is a self-balance between the biotic resources and their interactions both in quantitative and qualitative aspects and the balance is affected when new elements are introduced to the system. Especially, the elements introduced into the system have toxic properties; the aquatic system is under threat of quality degradation that seriously affects global biodiversity. Aquatic system today is polluted by industrial effluents comprising organic and synthetic chemical substances, heavy metals, dyes, oil etc; house hold wastes such as detergents and drainage; e-wastes, pesticides from agricultural lands, spillage of oil from ships etc. These pollutants of both biodegradable and non-biodegradable change the water qualities such as colour, odour, surface tension, thermal properties, conductivity, density, pH etc [1]. The acidic and alkali pollutants destroy most invertebrates and microorganisms [2]. Due to increased anthropogenic activities, aquatic organisms are continuously exposed to the elevated levels of metal concentration that the levels not previously encountered. ...

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Arsenic exposure alters hepatic arsenic species composition and stress-mediated gene expression in the common killifish (Fundulus heteroclitus)

Cited by 88 publications

References 53 publications

Estrogen receptor-related receptors in the killifish Fundulus heteroclitus: diversity, expression, and estrogen responsiveness

Estrogen receptor-related receptors in the killifish Fundulus heteroclitus: diversity, expression, and estrogen responsiveness

HISTOPATHOLOGICAL CHANGES IN LIVER AND GILLS OF Odontesthes bonariensis INHABITING A LAKE WITH HIGH CONCENTRATIONS OF ARSENIC AND FLUORIDE (CHASICÓ LAKE, BUENOS AIRES PROVINCE)

Environmental Health Monitoring: A Pragmatic Approach

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