Arsenic effect on the model crop symbiosis Bradyrhizobium–soybean

Talano, Melina A.; Cejas, Romina B.; González, Pablo J.; Agostini, Elizabeth

doi:10.1016/j.plaphy.2012.11.007

Cited by 40 publications

(21 citation statements)

References 29 publications

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“…TF assesses the ability of plants to transfer As from roots to leaves, whereas BCF is the ratio of concentrations in plant tissue to those in the soil [8,26,49]. TF and BCF were calculated as follows: TF = As concentration in leaves (mg kg ).…”

Section: Determinationsmentioning

confidence: 99%

“…Soils with high concentration of As negatively affect crop production and food safety, a phenomenon that has been documented in several countries [3,4]. Plants exposed to high As concentrations show reduced germination, decreased chlorophyll content and photosynthesis rate, reduced height, tillering and/or ramification, and decreased root and aerial biomass growth and yield; As also negatively affects the Bradyrhizobium-legume symbiosis, and may even cause death [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

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The Arbuscular Mycorrhiza Rhizophagus intraradices Reduces the Negative Effects of Arsenic on Soybean Plants

Spagnoletti

Lavado

2015

Agronomy

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Arsenic (As) in soils causes several detrimental effects, including death. Arsenic toxicity in soybean plants (Glycine max L.) has been little studied. Arbuscular mycorrhiza (AM) increase the tolerance of host plants to abiotic stress, like As. We investigated the effects of AM fungi on soybean grown in As-contaminated soils. A pot experiment was carried out in a glasshouse, at random with five replications. We applied three levels of As (0, 25, and 50 mg As kg −1 ), inoculated and non-inoculated with the AM fungus Rhizophagus intraradices (N.C. Schenck & G.S. Sm.) C. Walker & A. Schüßler. Plant parameters and mycorrhizal colonization were measured. Arsenic in the substrate, roots, and leaves was quantified. Arsenic negatively affected the AM percentage of spore germination and hyphal length. As also affected soybean plants negatively: an extreme treatment caused a reduction of more than 77.47% in aerial biomass, 68.19% in plant height, 78.35% in number of leaves, and 44.96% reduction in root length, and delayed the phenological evolution. Mycorrhizal inoculation improved all of these parameters, and decreased plant As accumulation (from 7.8 mg As kg ). AM inoculation showed potential to reduce As toxicity in contaminated areas. The AM fungi decreased As concentration in plants following different ways: dilution effect, less As intake by roots, and improving soybean tolerance to As.

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Section: Determinationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Arbuscular Mycorrhiza Rhizophagus intraradices Reduces the Negative Effects of Arsenic on Soybean Plants

Spagnoletti

Lavado

2015

Agronomy

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“…Being a toxic metalloid element, As(III) is reasonably considered as a repellent to most microorganisms2526. However, its role as an attractant to As(III)-oxidizing bacteria such as H. arsenicoxydans ULPAs120, Rhizobium sp.…”

Section: Discussionmentioning

confidence: 99%

Arsenite oxidation regulator AioR regulates bacterial chemotaxis towards arsenite in Agrobacterium tumefaciens GW4

Shi

Fan

Qiao

et al. 2017

Sci Rep

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Some arsenite [As(III)]-oxidizing bacteria exhibit positive chemotaxis towards As(III), however, the related As(III) chemoreceptor and regulatory mechanism remain unknown. The As(III)-oxidizing bacterium Agrobacterium tumefaciens GW4 displays positive chemotaxis towards 0.5–2 mM As(III). Genomic analyses revealed a putative chemoreceptor-encoding gene, mcp, located in the arsenic gene island and having a predicted promoter binding site for the As(III) oxidation regulator AioR. Expression of mcp and other chemotaxis related genes (cheA, cheY2 and fliG) was inducible by As(III), but not in the aioR mutant. Using capillary assays and intrinsic tryptophan fluorescence spectra analysis, Mcp was confirmed to be responsible for chemotaxis towards As(III) and to bind As(III) (but not As(V) nor phosphate) as part of the sensing mechanism. A bacterial one-hybrid system technique and electrophoretic mobility shift assays showed that AioR interacts with the mcp regulatory region in vivo and in vitro, and the precise AioR binding site was confirmed using DNase I foot-printing. Taken together, these results indicate that this Mcp is responsible for the chemotactic response towards As(III) and is regulated by AioR. Additionally, disrupting the mcp gene affected bacterial As(III) oxidation and growth, inferring that Mcp may exert some sort of functional connection between As(III) oxidation and As(III) chemotaxis.

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“…Because As(III) is a toxic metalloid, it is recognized as a repellent to some microorganisms, such as Bradyrhizobium japonicum E10 and Azospirillum brasilense Az39 (Talano et al, 2013;Armendariz et al, 2015). Interestingly, As(III) is an attractant to some As(III)oxidizing bacteria at low concentrations, such as A. tumefaciens GW4, H. arsenicoxydans ULPAs1 and Rhizobium sp.…”

Section: Correlation Between Arsenite Oxidation and Chemotaxismentioning

confidence: 99%

Microbial Oxidation of Arsenite: Regulation, Chemotaxis, Phosphate Metabolism and Energy Generation

Shi

Wang

2020

Front. Microbiol.

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Arsenic (As) is a metalloid that occurs widely in the environment. The biological oxidation of arsenite [As(III)] to arsenate [As(V)] is considered a strategy to reduce arsenic toxicity and provide energy. In recent years, research interests in microbial As(III) oxidation have been growing, and related new achievements have been revealed. This review focuses on the highlighting of the novel regulatory mechanisms of bacterial As(III) oxidation, the physiological relevance of different arsenic sensing systems and functional relationship between microbial As(III) oxidation and those of chemotaxis, phosphate uptake, carbon metabolism and energy generation. The implication to environmental bioremediation applications of As(III)-oxidizing strains, the knowledge gaps and perspectives are also discussed.

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Arsenic effect on the model crop symbiosis Bradyrhizobium–soybean

Cited by 40 publications

References 29 publications

The Arbuscular Mycorrhiza Rhizophagus intraradices Reduces the Negative Effects of Arsenic on Soybean Plants

The Arbuscular Mycorrhiza Rhizophagus intraradices Reduces the Negative Effects of Arsenic on Soybean Plants

Arsenite oxidation regulator AioR regulates bacterial chemotaxis towards arsenite in Agrobacterium tumefaciens GW4

Microbial Oxidation of Arsenite: Regulation, Chemotaxis, Phosphate Metabolism and Energy Generation

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