Arsenic-contaminated soils genetically modifiedPseudomonas spp. and their arsemc-phytoremediation potential

Сизова, О. И.; Кочетков, В. В.; Validov, Shamil; Boronin, A. M.; Kosterin, Paul; Lyubun, Yelena V.

doi:10.1007/bf02991246

Cited by 20 publications

(9 citation statements)

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“…Under ideal conditions, its biomass doubles in 24 h (Khondker, 2003). Moreover, arsenate is the predominant species in the oxic water and arsenate and arsenite are bioavailable forms to the plants in the aquatic systems (Sizova et al, 2002). The present study showed that S. polyrhiza L. accumulates a good amount of arsenic when exposed to arsenate.…”

Section: Potential Of S Polyrhiza L For the Phytoremediation Of Arssupporting

confidence: 50%

“…Aquatic macrophytes can be a good remediation option, because a few species have already been reported to accumulate arsenic from water (Robinson et al, 2003;Mkandawire and Dudel, 2005). Arsenate is the predominant species in the oxic water and arsenate and arsenite are bioavailable forms to the plants in the aquatic systems (Sizova et al, 2002). The dynamics of arsenate exchange between water and adsorbing colloids are analogous to those of phosphate, though the competition for exchange sites favors phosphate over arsenate (Mkandawire et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Arsenic accumulation in duckweed (Spirodela polyrhiza L.): A good option for phytoremediation

et al. 2007

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Some unavoidable drawbacks of traditional technologies have made phytoremediation a promising alternative for removal of arsenic from contaminated soil and water. In the present study, the potential of an aquatic macrophyte Spirodela polyrhiza L. for phytofiltration of arsenic, and the mechanism of the arsenic uptake were investigated. The S. polyrhiza L. were grown in three test concentrations of arsenate and dimethylarsinic acid (DMAA) (i.e. 1.0, 2.0 and 4.0 µM) with 0 (control), 100 or 500 µM of phosphate. One control treatment was also set for each test concentrations of arsenic.

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Section: Potential Of S Polyrhiza L For the Phytoremediation Of Arssupporting

confidence: 50%

Section: Introductionmentioning

confidence: 99%

Arsenic accumulation in duckweed (Spirodela polyrhiza L.): A good option for phytoremediation

et al. 2007

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“…Furthermore, growth reduction in As treated and low P or P-depleted media was most probably due to the reduction of enzyme activity (Jain and Gadre, 2004;Mishra and Dubey, 2006) and/ or due to Asinduced oxidative stress (Singh et al, 2007). Arsenic induces toxicity by attaching itself to the sulfhydryl group of root protein resulting in the disruption of root functions (Speer, 1973) and even cellular death (Sizova et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…They attack plant cell membranes causing an inhibition of cellular function and death (Sizova et al, 2002). Many studies have been done by adding P in the presence of arsenite or arsenate to soil or under hydroponic conditions.…”

Section: Introductionmentioning

confidence: 99%

Effect of phosphorus on the concentrations of arsenic, iron and some other elements in barley grown hydroponically

Shaibur

Adjadeh

Kawai

2013

J. Soil Sci. Plant Nutr.

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Hydroponic barley (Hordeum vulgare L. cv. minorimugi) was treated with 10 µM arsenic (As) together with 500, 250, 50 and 0 µM phosphorus (P) for 14 days to observe the response of the seedlings. The plants were also treated with 0 µM As + 0 µM P. Iron (Fe)-plaque was visible in the roots under As-treated and P-depleted conditions. The intensity of reddish coloration in the roots decreased with increasing P in the media. However, reddish coloration was not found in the absence of both As and P. The results show that both As and P played a vital role in the formation of Fe-plaque. Additionally, Fe-plaque formation was dependent on the concentration of P in the media. Iron concentration in the roots of the As treated plants without P was higher than that observed in the roots treated with 0 µM As + 0 µM P which suggests that As may have been adsorbed with Fe on the surface of roots, forming Fe-plaque. Arsenic concentration in the shoots and the roots was low under 500 and 250 µM P conditions while high under 50 and 0 µM P conditions. It was found that a higher concentration of P (50 µM; 5 times of As level) was required in the medium to reduce As concentration in the plant tissues.

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“…We used strains P. fluorescens M4-80 , P. fluorescens Pf-5 [Howell and Stipanovic, 1979], P. fluorescens Q2-87 [Vincent et al, 1991], P. fluorescens Q8r1 96 [Raaijmakers and Weller, 1998], P. fluorescens 38a [Sizova et al, 2002], P. fluorescens 2-79 [Thomashow and Weller, 1988] and P. putida KT2440 [Regenhardt et al, 2002] [Woodcock et al, 1989] was used for the cloning and isolation of plasmids.…”

Section: Bacterial Strainsmentioning

confidence: 99%

Gene Expression of Lytic Endopeptidases AlpA and AlpB from <b><i>Lysobacter </i></b>sp. XL1 in Pseudomonads

Tsfasman

Lapteva²,

Krasovskaya³

et al. 2015

Microb Physiol

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Development of an efficient expression system for (especially secreted) bacterial lytic enzymes is a complicated task due to the specificity of their action. The substrate for such enzymes is peptidoglycan, the main structural component of bacterial cell walls. For this reason, expression of recombinant lytic proteins is often accompanied with lysis of the producing bacterium. This paper presents data on the construction of an inducible system for expression of the lytic peptidases AlpA and AlpB from Lysobacter sp. XL1 in Pseudomonas fluorescens Q2-87, which provides for the successful secretion of these proteins into the culture liquid. In this system, the endopeptidase gene under control of the T7lac promoter was integrated into the bacterial chromosome, as well as the Escherichia coli lactose operon repressor protein gene. The T7 pol gene under lac promoter control, which encodes the phage T7 RNA polymerase, is maintained in Pseudomonas cells on the plasmids. Media and cultivation conditions for the recombinant strains were selected to enable the production of AlpA and AlpB by a simple purification protocol. Production of recombinant lytic enzymes should contribute to the development of new-generation antimicrobial drugs whose application will not be accompanied by selection of resistant microorganisms.

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Arsenic-contaminated soils genetically modifiedPseudomonas spp. and their arsemc-phytoremediation potential

Cited by 20 publications

References 10 publications

Arsenic accumulation in duckweed (Spirodela polyrhiza L.): A good option for phytoremediation

Arsenic accumulation in duckweed (Spirodela polyrhiza L.): A good option for phytoremediation

Effect of phosphorus on the concentrations of arsenic, iron and some other elements in barley grown hydroponically

Gene Expression of Lytic Endopeptidases AlpA and AlpB from <b><i>Lysobacter </i></b>sp. XL1 in Pseudomonads

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