Arrhythmias in Thyroid Disorders

Meziou, Sonia Marrakchi; Bennour, E.; Kanoun, F.; Marrakchi, Dania Idriss; Kammoun, Ikram; Kachboura, Salem

doi:10.1007/978-3-030-36871-5_20

Cited by 1 publication

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“…Alpha-MAs contain two cyclopropane rings within their R 1 chain, whereas keto-and methoxy-MAs contain one cyclopropane ring in the R 1 chain in addition to a ketone or methoxy functional group, respectively. 55 Cyclopropane ring locations are counted from the C1 position at which branching to R 1 and R 2 groups occurs. For this example, deprotonated α-MA (C-80(c16Z, c32Z)) containing two cyclopropane rings was subjected to 213 nm UVPD (Figure 2).…”

Section: Resultsmentioning

confidence: 99%

Localization of Cyclopropane Modifications in Bacterial Lipids via 213 nm Ultraviolet Photodissociation Mass Spectrometry

2019

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Subtle structural features in bacterial lipids such as unsaturation elements can have vast biological implications. Cyclopropane rings have been correlated with tolerance to a number of adverse conditions in bacterial phospholipids. They have been shown to play a major role in Mycobacterium tuberculosis (M. tuberculosis or Mtb) pathogenesis as they occur in mycolic acids (MAs) in the mycobacterial cell. Traditional collisional activation methods allow elucidation of basic structural features of lipids but fail to reveal the presence and position of cyclopropane rings. Here, we employ 213 nm ultraviolet photodissociation mass spectrometry (UVPD-MS) for structural characterization of cyclopropane rings in bacterial phospholipids and MAs. Upon UVPD, dual cross-ring CC cleavages on both sides of the cyclopropane ring are observed for cyclopropyl lipids, resulting in diagnostic pairs of fragment ions spaced 14 Da apart, thus enabling cyclopropane localization. These diagnostic pairs of ions corresponding to dual cross-ring cleavage are observed in both negative and positive ion modes and afford localization of multiple cyclopropane rings within a single lipid. This method was integrated with liquid chromatography (LC) for LC/UVPD-MS analysis of cyclopropyl glycerophospholipids in Escherichia coli (E. coli) and for analysis of MAs in Mycobacterium bovis (M. bovis) and M. tuberculosis lipid extracts.

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Section: Resultsmentioning

confidence: 99%