Arrhenius Plot Analysis, Temperature Coefficient and Q10 Value Estimation for the Effect of Temperature on the Growth Rate on Acrylamide by the Antarctic Bacterium Pseudomonas sp. Strain DRYJ7

Abstract

Several models are available to model the effect of temperature on the growth rate of microorganism on substrates. One of the models is Arrhenius and is very popular due to it having few parameters. An apparent activation energy based on the Arrhenius plot on the growth on acrylamide by Pseudomonas sp. strain DRYJ7 on acrylamide is reported for the first time. The bacterium was grown in minimal salts media supplemented with 1000 mg/L of acrylamide as a nitrogen source and glucose as the carbon source. The plot of ln m (specific growth rate) against 1/T for growth on acrylamide was carried out spanning the range of temperature from 10 to 35 oC. Regression analysis from 10–20 oC results in an activation energy of 14.96 kJ mol-1. A relative 10 ˚C increase in the surrounding temperature, usually results in doubling the reaction rate, with corresponding Q10 value of 1.8, which is the approximate value for a number of biological reactions. The Q10 values, determined from the Arrhenius plot of 2.17 and a theta value of 1.03 obtained in this work, are within the normal range for many biological values. This is the first time that values for the activation energy, Q10 and theta for the growth of a bacterium on acrylamide is reported.

Activation energy, Temperature Coefficient and Q10 Value Estimations of the Growth of Alcaligenes sp. YLA11 on Diesel

“…[25] whereas a Q10 value of 2.17 was determined to be appropriate for the growth rate of this organism when fed diesel. In another study on the biodegradation of acrylamide, the Q10 value was reported to be 2.17, which is lower [21] According to our calculations (Fig. 3), the theta value of the bacterium's molybdenum reduction was equivalent to that of our previous theta value estimate of 1.08 for Serratia sp.…”

“…strain DRYJ7 grew at a rate of 1.03 on acrylamide, according to a study. Theta was found to be a value of 1.03 [21]. There have been reports of xenobiotic degradation theta values as high as 16.2, however the theta value here is between 1.1 and 1.7, which is common for many biological processes [55].…”

“…It was previously found that Pseudomonas sp. DrYJ7's growth rate on another xenobiotic; acrylamide had an activation energy of 14.96 kJ/mol between 10 and 20 °C [21], which is much lower. For various xenobiotic biodegradations, our Arrhenius model activation energy was within the published literature's range (Table 2).…”

“…It has been shown in a variety of conditions using a variety of substrates, such as the deolorization of various dyes, that the activation energy, which is dependent on the change in temperature, plays an important role in the metabolic activity of microorganisms. This has been demonstrated both theoretically and experimentally [19,[35][36][37][38], chromate reduction [32,33] and phenolics biodegradation [18,[25][26][27][28] and acrylamide [21]. Although the specifics of the process that underlies the shift are not yet known, there are two hypotheses that offer two possible explanations for the phenomenon.…”

“…Arrhenius plot analysis and the effect of temperature on Pseudomonas sp. strain DRYJ7's acrylamide growth were the subject of a similar study a number of years ago [21].…”