ARRDC5 expression is conserved in mammalian testes and required for normal sperm morphogenesis

Giassetti, Mariana Ianello; Miao, De-Qiang; Law, Nathan C.; Oatley, Melissa J.; Park, Julie; Robinson, LeeLa D.; Maddison, Lisette A.; Bernhardt, Miranda L.; Oatley, Jon M.

doi:10.1038/s41467-023-37735-y

Cited by 10 publications

(1 citation statement)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Spata31 ‐deficient male mice exhibited a low sperm count and premature shedding of germ cells into the lumen, ultimately causing azoospermia and male sterility 37 . ARRDC5 expression is conserved in mammalian testes and is required for normal sperm morphogenesis 38 . OAZ3 is essential for the rigid connection of sperm tails to heads in mice 39 .…”

Section: Discussionmentioning

confidence: 99%

Elevated Id2 expression causes defective meiosis and spermatogenesis in mice

He,

Yan,

Shang

et al. 2023

Developmental Dynamics

View full text Add to dashboard Cite

BackgroundInhibitors of DNA binding (ID) proteins mainly inhibit gene expression and regulate cell fate decisions by interacting with E‐proteins. All four ID proteins (ID1–4) are present in the testis, and ID4 has a particularly important role in spermatogonial stem cell fate determination. Several lines of evidence indicate that ID proteins are involved in meiosis; however, functional experiments have not been conducted to validate this observation.ResultsIn this study, we report that ID2 is enriched in spermatocytes and that forced ID2 expression in germ cells causes defects in spermatogenesis. A detailed analysis demonstrated that Id2 overexpression (Id2 OE) decreased the total number of spermatogonia and changed the dynamics of meiosis progression. Specifically, spermatocytes were enriched in the zygotene stage, and the proportion of pachytene spermatocytes was significantly decreased, indicating defects in the zygotene–pachytene transition. The number of MLH1‐positive foci per cell was decreased in pachytene spermatocytes from Id2 OE testes, suggesting abnormalities in recombination. Transcriptome analysis revealed that forced Id2 expression changed the expression of a list of genes mainly associated with meiosis and spermatid development.ConclusionsID2 protein is expressed in spermatocytes, and its genetic ablation in the germline does not affect spermatogenesis, likely due to genetic compensation of its family members. However, forced Id2 expression changes meiosis progression and causes defects in spermiogenesis. These data provide important evidence that ID proteins play pivotal roles in male meiosis and spermatid development.

show abstract

Section: Discussionmentioning

confidence: 99%

Elevated Id2 expression causes defective meiosis and spermatogenesis in mice

He,

Yan,

Shang

et al. 2023

Developmental Dynamics

View full text Add to dashboard Cite

show abstract

Spem2, a novel testis-enriched gene, is required for spermiogenesis and fertilization in mice

Li,

Shen,

Xiong

et al. 2024

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

Spermiogenesis is considered to be crucial for the production of haploid spermatozoa with normal morphology, structure and function, but the mechanisms underlying this process remain largely unclear. Here, we demonstrate that SPEM family member 2 (Spem2), as a novel testis-enriched gene, is essential for spermiogenesis and male fertility. Spem2 is predominantly expressed in the haploid male germ cells and is highly conserved across mammals. Mice deficient for Spem2 develop male infertility associated with spermiogenesis impairment. Specifically, the insufficient sperm individualization, failure of excess cytoplasm shedding, and defects in acrosome formation are evident in Spem2-null sperm. Sperm counts and motility are also significantly reduced compared to controls. In vivo fertilization assays have shown that Spem2-null sperm are unable to fertilize oocytes, possibly due to their impaired ability to migrate from the uterus into the oviduct. However, the infertility of Spem2−/− males cannot be rescued by in vitro fertilization, suggesting that defective sperm–egg interaction may also be a contributing factor. Furthermore, SPEM2 is detected to interact with ZPBP, PRSS21, PRSS54, PRSS55, ADAM2 and ADAM3 and is also required for their processing and maturation in epididymal sperm. Our findings establish SPEM2 as an essential regulator of spermiogenesis and fertilization in mice, possibly in mammals including humans. Understanding the molecular role of SPEM2 could provide new insights into future therapeutic treatment of human male infertility and development of non-hormonal male contraceptives.

show abstract

Fertilization by short-term stored sperm alters DNA methylation patterns at single-base resolution in common carp (Cyprinus carpio) embryos

Cheng,

Zhang,

Nayak

et al. 2024

Rev Fish Biol Fisheries

View full text Add to dashboard Cite

Sperm after short-term storage in vitro is widely used for artificial fertilization in aquaculture. It has been shown that short-term storage affects sperm motility characteristics, resulting in diminished fertility. However, the detrimental effects of short-term sperm storage on embryos development have remained unexplored in single-base methylome resolution. The main aim of the present study was to investigate DNA methylation in the offspring of common carp (Cyprinus carpio) derived from short-term stored sperm. Sperm were stored in artificial seminal plasma on ice (0–2 °C) for 0, 3 and 6 days in vitro, fertilization was performed using oocytes from a single female, and embryos were collected at the mid-blastula stage. In the DNA methylation study, DNA from both sperm and embryos was extracted and analysed using liquid chromatography with tandem mass spectrometry (LC–MS/MS). Concurrently, DNA methylation levels of embryos in single base were evaluated through whole genome bisulfite sequencing (WGBS). Sperm storage showed negative effects on sperm motility, viability, and DNA integrity, but had no effect on global DNA methylation of spermatozoa and resulting embryos. Results from the WGBS showed that methylation of 3313 differentially methylated regions (DMRs)-target genes was affected in the embryos fertilized with the 6-day-stored sperm, and the identified DMRs were mainly involved in cell adhesion, calcium, mitogen-activated protein kinase and adrenergic signalling, melanogenesis, metabolism and RNA transport. Such results suggest that prolongation of storage time may have certain impacts on embryonic development. These initial results provide valuable information for future consideration of the DNA methylome in embryos generated from short-term stored sperm, which are used for genetic management of broodstock in aquaculture.

show abstract

ARRDC5 expression is conserved in mammalian testes and required for normal sperm morphogenesis

Cited by 10 publications

References 67 publications

Elevated Id2 expression causes defective meiosis and spermatogenesis in mice

Elevated Id2 expression causes defective meiosis and spermatogenesis in mice

Spem2, a novel testis-enriched gene, is required for spermiogenesis and fertilization in mice

Fertilization by short-term stored sperm alters DNA methylation patterns at single-base resolution in common carp (Cyprinus carpio) embryos

Contact Info

Product

Resources

About