Array-Based Transcript Profiling and Limiting-Dilution Reverse Transcription-PCR Analysis Identify Additional Latent Genes in Kaposi's Sarcoma-Associated Herpesvirus

Genomewide Mapping and Screening of Kaposi's Sarcoma-Associated Herpesvirus (KSHV) 3′ Untranslated Regions Identify Bicistronic and Polycistronic Viral Transcripts as Frequent Targets of KSHV MicroRNAs

“…S2 in the supplemental material). One of the 3=UTRs spans the intergenic region and the entire ORF4 coding region, while the second one is a spliced 3=UTR derived from sequence immediately after the stop codon to the poly(A) tail, which has been previously described (63,64). Thus, ORF-K1 has long and short 3=UTRs, which might subject them to differential regulation by miRNAs or RNA-binding proteins in different stages of the KSHV life cycle.…”

Active lytic infection of human primary tonsillar B cells by KSHV and its noncytolytic control by activated CD4+ T cells

“…Experimental samples (labeled with Cyanine 5-CTP) and reference samples (labeled with Cyanine 3-CTP) were competitively hybridized to custom viral microarrays (Agilent) according to the manufacturer's instructions. The design of the viral microarrays has been previously described (63) and can be obtained from Agilent (Amadid 029238). Washed and processed arrays were scanned using the GenePix 4000B Scanner (Axon Instruments) and the feature intensities extracted using GenePix Pro 6.0 software.…”

KSHV and the pathogenesis of Kaposi sarcoma: listening to human biology and medicine

“…Finally, very recent evidence suggests that a small number of genes outside the major latency locus may also be expressed in latency (158). Chief among these is ORF-K1, a transmembrane signaling molecule whose activity mimics signaling via the B cell antigen receptor (159).…”