Array-based analysis of gene expression in childhood acute lymphoblastic leukemia

Bruchova, Hana; Kalinová, Markéta; Brdička, R

doi:10.1016/s0145-2126(03)00120-6

Cited by 11 publications

(3 citation statements)

References 24 publications

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“…In contrast to our findings Niemeyer et al observed frequent expression of SSX-1 and SXX-2 in ALL blasts [9]. ERCC1, upregulated in ALL cells after CD40 stimulation, is considered to be upregulated in childhood ALL de novo cases [31]. According to the authors of this report PCNA and ERCC1 might contribute to development of the disease.…”

Section: Discussioncontrasting

confidence: 99%

Acute lymphoblastic leukemia-derived dendritic cells express tumor associated antigens: PNPT1, PMPCB, RHAMM, BSG and ERCC1.

Łuczyński¹,

Kowalczuk²,

Stasiak-Barmuta³

et al. 2009

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In all types of leukemia both in children and adults there is a need for novel therapies that could reduce the risk of relapse after standard treatment. Acute lymphoblastic leukemia (ALL) cells are ineffective antigen presenting cells, but as shown by many authors including results from our laboratory, stimulation with CD40L restores their antigen expressing capacity. The development of T-cell therapies for leukemic patients can be based on discovery of leukemia-associated antigens (LAA) which could be recognized by the host immune system. The aim of our present study was to test the hypothesis that leukemia-derived dendritic cells maintain the expression of tumor associated antigens. Twenty five children with B-cell precursor ALL were prospectively enrolled into the study. The mononuclear cells from peripheral blood or bone marrow were cultured and stimulated (or not) with CD40L and IL-4. The assessment of costimulatory/adhesion molecules with the use of flow cytometry and real-time RT PCR were used to confirm the possibility of turning ALL cells into dendritic-like cells. Additionally 22 tumor associated antigens mRNA levels were determined by real-time PCR technique with the TaqMan chemistry using ready-to-use Low Density Arrays for Gene Expression. The results of the study showed maintained expression and even up-regulation of some (PNPT1, PMPCB, HMMR/RHAMM, BSG and ERCC1) tumor associated antigens in CD40-activated leukemic cells. CD40L stimulation leading to the differentiation of leukemic cells into DCs which combine both antigen presenting function and expression of tumor associated antigens represents an interesting approach in cancer immunotherapy.

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Section: Discussioncontrasting

confidence: 99%

Acute lymphoblastic leukemia-derived dendritic cells express tumor associated antigens: PNPT1, PMPCB, RHAMM, BSG and ERCC1.

Łuczyński¹,

Kowalczuk²,

Stasiak-Barmuta³

et al. 2009

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“…Under pathological conditions the situation is more complex as the gene expression profiles and signaling pathways may be altered [22, 23]. In KG-1 cells, the protein kinase C (PKC) pathway is an important signal transduction route for DLC activation and inhibition of PKC may abort DLC induction [10].…”

Section: Discussionmentioning

confidence: 99%

A Rapid Culture Technique Produces Functional Dendritic‐Like Cells from Human Acute Myeloid Leukemia Cell Lines

Ning

Morgan

Pamphilon

2011

BioMed Research International

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Most anti-cancer immunotherapeutic strategies involving dendritic cells (DC) as vaccines rely upon the adoptive transfer of DC loaded with exogenous tumour-peptides. This study utilized human acute myeloid leukemia (AML) cells as progenitors from which functional dendritic-like antigen presenting cells (DLC) were generated, that constitutively express tumour antigens for recognition by CD8+ T cells. DLC were generated from AML cell lines KG-1 and MUTZ-3 using rapid culture techniques and appropriate cytokines. DLC were evaluated for their cell-surface phenotype, antigen uptake and ability to stimulate allogeneic responder cell proliferation, and production of IFN-γ; compared with DC derived from normal human PBMC donors. KG-1 and MUTZ-3 DLC increased expression of CD80, CD83, CD86, and HLA-DR, and MUTZ-3 DLC downregulated CD14 and expressed CD1a. Importantly, both KG-1 and MUTZ-3-derived DLC promoted proliferation of allogeneic responder cells more efficiently than unmodified cells; neither cells incorporated FITC-labeled dextran, but both stimulated IFN-γ production from responding allogeneic CD8+ T cells. Control DC produced from PBMC using the FastDC culture also expressed high levels of critical cell surface ligands and demonstrated good APC function. This paper indicates that functional DLC can be cultured from the AML cell lines KG-1 and MUTZ-3, and FastDC culture generates functional KG-1 DLC.

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“…Similarly, analysis of patients with completely resected non-small-cell lung cancer showed that ERCC1-negative tumors appear to benefit from adjuvant cisplatin-based chemotherapy, whereas patients with ERCC1-positive tumors do not [22]. Finally, increased ERCC1 expression together with PCNA was observed in childhood acute lymphoblastic leukemia (ALL) [23].…”

Section: Discussionmentioning

confidence: 99%

High expression ofERCC1,FLT1,NME4andPCNAassociated with poor prognosis and advanced stages in myelodysplastic syndrome

Kracmarova

Čermák

Brdička

et al. 2008

Leukemia & Lymphoma

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Myelodysplastic syndrome (MDS) represents a good model for research of prognostic/progression markers due to frequent transformation into acute myeloid leukemia (AML). We analysed expression profiles of 26 MDS and 6 AML patients using cDNA arrays comprising 588 gene probes. The array data were validated in a larger set of 46 patients by qRT-PCR. Data analysis identified differently expressed genes in MDS and the cluster of four genes (ERCC1, FLT1, NME4 and PCNA) whose expression was correlated with MDS subtypes. High expression of these genes was associated with poor prognosis and/or unfavorable outcome. Furthermore, PCNA expression was correlated with peripheral blood blast percentage (r = 0.71, p < 0.05), while the other genes showed non-significant correlation. Our findings demonstrate the progressive up-regulation of the genes along the sequence of 5q-syndrome/RCMD/RAEB/de novo AML, suggesting their association with disease progression.

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Array-based analysis of gene expression in childhood acute lymphoblastic leukemia

Cited by 11 publications

References 24 publications

Acute lymphoblastic leukemia-derived dendritic cells express tumor associated antigens: PNPT1, PMPCB, RHAMM, BSG and ERCC1.

Acute lymphoblastic leukemia-derived dendritic cells express tumor associated antigens: PNPT1, PMPCB, RHAMM, BSG and ERCC1.

A Rapid Culture Technique Produces Functional Dendritic‐Like Cells from Human Acute Myeloid Leukemia Cell Lines

High expression ofERCC1,FLT1,NME4andPCNAassociated with poor prognosis and advanced stages in myelodysplastic syndrome

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