Arrangements of Mobile Genetic Elements among Virotype E Subpopulation of
            <i>Escherichia coli</i>
            Sequence Type 131 Strains with High Antimicrobial Resistance and Virulence Gene Content

Pajand, Omid; Rahimi, Hamzeh; Darabi, Narges; Roudi, Solaleh; Ghassemi, Khatereh; Aarestrup, Frank Møller; Leekitcharoenphon, Pimlapas

doi:10.1128/msphere.00550-21

Cited by 10 publications

(15 citation statements)

References 42 publications

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“…Isolates with these features were previously reported by Chen et al 7 among bacteremia isolates collected in 2015 in South East Asia and associated with increased virulence and AMR. Likewise, Pajand et al described such clade C2 isolates among ST131 isolates from Iran 38 . An apparent stable integration of blaCTX-M-15 and aac(6')-Ib-cr might have contributed to its success: in clade C2, the ciprofloxacin-inactivating AAC(6')-Ib-cr was shown to confer a selective advantage in the presence of ciprofloxacin over isolates that contained QRDR mutations alone 39 .…”

Section: Discussionmentioning

confidence: 98%

Convergence of virulence and antimicrobial resistance in increasingly prevalent Escherichia coli ST131 papGII+ sublineages

et al. 2022

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Escherichia coli lineage ST131 is an important cause of urinary tract and bloodstream infections worldwide and is highly resistant to antimicrobials. Specific ST131 lineages carrying invasiveness-associated papGII pathogenicity islands (PAIs) were previously described, but it is unknown how invasiveness relates to the acquisition of antimicrobial resistance (AMR). In this study, we analysed 1638 ST131 genomes and found that papGII+ isolates carry significantly more AMR genes than papGII-negative isolates, suggesting a convergence of virulence and AMR. The prevalence of papGII+ isolates among human clinical ST131 isolates increased dramatically since 2005, accounting for half of the recent E. coli bloodstream isolates. Emerging papGII+ lineages within clade C2 were characterized by a chromosomally integrated blaCTX-M-15 and the loss and replacement of F2:A1:B- plasmids. Convergence of virulence and AMR is worrying, and further dissemination of papGII+ ST131 lineages may lead to a rise in severe and difficult-to-treat extraintestinal infections.

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Section: Discussionmentioning

confidence: 98%

Convergence of virulence and antimicrobial resistance in increasingly prevalent Escherichia coli ST131 papGII+ sublineages

et al. 2022

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“…Located on plasmids with sequence similarities (95-100%), different carbapenemases of ST131 have been detected in other international clones as well [84][85][86]. Additionally, the co-carriage of bla OXA-1 , bla CTX-M-15 , aac6-Ib-cr and aac3-IIa has also been detected in strains of C2 clade [58,87].…”

Section: Cc/st131 the Worldwide Predominant High-risk Clonementioning

confidence: 96%

“…Initially, H30 was the most prevalent among them, emerging in the 1980s. In the course of clonal expansion, it obtained high-level fluoroquinolone resistance by sequential chromosomal mutations of gyrA and parC genes, then it also became resistant against beta-lactams by acquisition of plasmid-mediated ESBLs, as well as carbapenemases [15,29,45,52,53,58]. The self-transmissible plasmids of ST131 are characterised by a remarkable genetic diversity (plasmidome), they belong in particular to incompatibility group F (IncF type).…”

Section: Cc/st131 the Worldwide Predominant High-risk Clonementioning

confidence: 99%

“…The most frequently reported plasmid MLST types are F1:A2:B20 in multidrug-resistant (MDR) clade C1 and F2:A1:B of MDR clade C2 [15,78,79]. A recent in-depth analysis showed that by a novel subset of C2, the plasmidome was not uniform; it had a combined pattern of certain plasmid types, and it showed a homogeneous replicon structure of F31/F36:A4:B1 [58].…”

Section: Cc/st131 the Worldwide Predominant High-risk Clonementioning

confidence: 99%

“…Based on the PCR-verified presence of certain virulence genes including Afa and Dr adhesins (afa/draBC), operon (afa), catecholate siderophore receptor (iroN), secreted autotransporter toxin (sat), ibeA ('invasion of brain endothelium' gene), allele II and III of papG gene (papGII and papGIII), cytotoxic necrotising factor type 1 (cnf1), alpha-hemolysin (hlyA), cytolethal distending toxin (cdtB) and K1 variant of group II capsule (neuC-K1) virotypes are defined from A to E groups [52]. The current evolution of new virotypes in C2 subclade can also be seen, as a study from Singapore demonstrated a monophyletic subclone from bacteraemia referred to as SEA-C2 [58,91].…”

Section: Cc/st131 the Worldwide Predominant High-risk Clonementioning

confidence: 99%

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Emergence and Dissemination of Extraintestinal Pathogenic High-Risk International Clones of Escherichia coli

Kocsis

Gulyás

Szabó

2022

Life

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Multiresistant Escherichia coli has been disseminated worldwide, and it is one of the major causative agents of nosocomial infections. E. coli has a remarkable and complex genomic plasticity for taking up and accumulating genetic elements; thus, multiresistant high-risk clones can evolve. In this review, we summarise all available data about internationally disseminated extraintestinal pathogenic high-risk E. coli clones based on whole-genome sequence (WGS) data and confirmed outbreaks. Based on genetic markers, E. coli is clustered into eight phylogenetic groups. Nowadays, the E. coli ST131 clone from phylogenetic group B2 is the predominant high-risk clone worldwide. Currently, strains of the C1-M27 subclade within clade C of ST131 are circulating and becoming prominent in Canada, China, Germany, Hungary and Japan. The C1-M27 subclade is characterised by blaCTX-M-27. Recently, the ST1193 clone has been reported as an emerging high-risk clone from phylogenetic group B2. ST38 clone carrying blaOXA-244 (a blaOXA-48-like carbapenemase gene) caused several outbreaks in Germany and Switzerland. Further high-risk international E. coli clones include ST10, ST69, ST73, ST405, ST410, ST457. High-risk E. coli strains are present in different niches, in the human intestinal tract and in animals, and persist in environment. These strains can be transmitted easily within the community as well as in hospital settings. WGS analysis is a useful tool for tracking the dissemination of resistance determinants, the emergence of high-risk mulitresistant E. coli clones and to analyse changes in the E. coli population on a genomic level.

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Identification of metallo-β-lactamases and AmpC production among Escherichia coli strains isolated from hemodialysis patients with urinary tract infection

et al. 2021

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Background This study aimed to identify metallo-β-lactamases (MBLs) and AmpC β-lactamases-producing Escherichia coli isolates obtained from hemodialysis (HD) patients with urinary tract infections (UTI). Methods and results A total of 257 HD patients with UTI were included in this study, from which 47 E. coli isolates were collected. Antibiotic susceptibility was tested by disc diffusion method. MBLs and AmpC production were phenotypically detected by imipenem-ethylenediaminetetracetate and cefoxitin/boronic acid assays, respectively. The presence of MBLs and AmpC genes was examined by polymerase chain reaction (PCR). Fosfomycin and ampicillin were the most and the least effective antibiotics against E. coli isolates, respectively. Moreover, 61.7% (29/47) of E. coli isolates were multidrug-resistant with seven different antibiotypes. Antibiotype V (AMP–CIP–IMP–MEM–CPD–CRO–CTX–GEN–LEV–SXT–TOB) was the most prevalent profile. Besides, 24 (51.1%) isolates were simultaneously resistant to imipenem and meropenem. Phenotypic assay showed MBL production in 16 (66.7%) of the 24 carbapenem-resistant E. coli isolates. The distribution of MBL genes in carbapenem-resistant E. coli was as follows: bla IMP 18 (72%), bla VIM 7 (28%), and bla NDM 1 (4%). AmpC was detected in 61.7% (29/47) of the isolates using the phenotypic method. The presence of AmpC genes was confirmed by PCR in only 26 of 29 (86.7%) AmpC producers. The frequencies of bla DHA-1 , bla ACC , and bla CMY-2 were 6 (20.7%), 11 (37.9%), and 21 (72.4%), respectively. Conclusions The emergence of MBL and AmpC coproducing E. coli isolates calls for an urgent surveillance program for timely diagnosis and screening of these genes in our healthcare systems.

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Arrangements of Mobile Genetic Elements among Virotype E Subpopulation of Escherichia coli Sequence Type 131 Strains with High Antimicrobial Resistance and Virulence Gene Content

Abstract: Escherichia coli sequence type 131 (ST131) is a globally dominant multidrug-resistant clone that is commonly associated with extraintestinal infections. Specific sublineages have been shown to have emerged and spread within ST131, highlighting the complex nature of ST131 epidemiology.

Cited by 10 publications

References 42 publications

Convergence of virulence and antimicrobial resistance in increasingly prevalent Escherichia coli ST131 papGII+ sublineages

Convergence of virulence and antimicrobial resistance in increasingly prevalent Escherichia coli ST131 papGII+ sublineages

Emergence and Dissemination of Extraintestinal Pathogenic High-Risk International Clones of Escherichia coli

Identification of metallo-β-lactamases and AmpC production among Escherichia coli strains isolated from hemodialysis patients with urinary tract infection

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