Aronia melanocarpa Extract Fermented by Lactobacillus plantarum EJ2014 Modulates Immune Response in Mice

Ali, Muhammad; Lee, Eon‐Bee; Lee, Seung-Jin; Lee, Sam-Pin; Boby, Naila; Suk, Kyoungho; Birhanu, Biruk Tesfaye; Park, Seung Chun

doi:10.3390/antiox10081276

Cited by 9 publications

(6 citation statements)

References 73 publications

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“…The levels of pro-inflammatory cytokines IFN-α, IFN-β, and IL-6 were significantly reduced in cells treated with the supernatant of L. plantarum Probio-88 strain [ 25 ]. In addition, the SlpA protein derived from L. acidophilus CICC 6074 [ 26 ], exopolysaccharide derived from L. plantarum L-14 strain [ 27 ], and Aronia Melanocarpa fruit fermented with L. plantarum EJ2014 [ 28 ] significantly reduced inflammation in an LPS-induced RAW 264.7 cell line model. Clinical data from infected patients show that SARS-CoV-2 is associated with a cytokine storm, which is one of the leading causes of death caused by the early elevation of systemic IL-10.…”

Section: Resultsmentioning

confidence: 99%

Mucosal immunization with Lactiplantibacillus plantarum-displaying recombinant SARS-CoV-2 epitopes on the surface induces humoral and mucosal immune responses in mice

et al. 2023

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Background The use of probiotic lactic acid bacteria as a mucosal vaccine vector is considered a promising alternative compared to the use of other microorganisms because of its “Generally Regarded as Safe” status, its potential adjuvant properties, and its tolerogenicity to the host. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes coronavirus disease (COVID-19), is highly transmissible and pathogenic. This study aimed to determine the potential of Lactiplantibacillus plantarum expressing SARS-CoV-2 epitopes as a mucosal vaccine against SARS-CoV-2. Results In this study, the possible antigenic determinants of the spike (S1–1, S1–2, S1–3, and S1–4), membrane (ME1 and ME2), and envelope (E) proteins of SARS-CoV-2 were predicted, and recombinant L. plantarum strains surface-displaying these epitopes were constructed. Subsequently, the immune responses induced by these recombinant strains were compared in vitro and in vivo. Most surface-displayed epitopes induced pro-inflammatory cytokines [tumor necrosis factor alpha (TNF-α and interleukin (IL)-6] and anti-inflammatory cytokines (IL-10) in lipopolysaccharide-induced RAW 264.7, with the highest anti-inflammatory to pro-inflammatory cytokine ratio in the S1–1 and S1–2 groups, followed by that in the S1–3 group. When orally administered of recombinant L. plantarum expressing SARS-CoV-2 epitopes in mice, all epitopes most increased the expression of IL-4, along with induced levels of TNF-α, interferon-gamma, and IL-10, specifically in spike protein groups. Thus, the surface expression of epitopes from the spike S1 protein in L. plantarum showed potential immunoregulatory effects, suggesting its ability to potentially circumvent hyperinflammatory states relevant to monocyte/macrophage cell activation. At 35 days post immunization (dpi), serum IgG levels showed a marked increase in the S1–1, S1–2, and S1–3 groups. Fecal IgA levels increased significantly from 21 dpi in all the antigen groups, but the boosting effect after 35 dpi was explicitly observed in the S1–1, S1–2, and S1–3 groups. Thus, the oral administration of SARS-CoV-2 antigens into mice induced significant humoral and mucosal immune responses. Conclusion This study suggests that L. plantarum is a potential vector that can effectively deliver SARS-CoV-2 epitopes to intestinal mucosal sites and could serve as a novel approach for SARS-CoV-2 mucosal vaccine development.

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Section: Resultsmentioning

confidence: 99%

Mucosal immunization with Lactiplantibacillus plantarum-displaying recombinant SARS-CoV-2 epitopes on the surface induces humoral and mucosal immune responses in mice

et al. 2023

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“…Thus, the fermentation of Aronia melanocarpa extract by L. plantarum was investigated to produce GABA, polyphenol, and flavonoid compounds. The fermented extract was shown to exert AI effects inhibiting the production of proinflammatory cytokines in RAW 264.7 cells and modulating the immune response in mice [54]. Furthermore, several molecules derived from the fermentation of red fruit juices have been studied for their AI effects.…”

Section: Anti-inflammatory Activitymentioning

confidence: 99%

Lactobacillus Use for Plant Fermentation: New Ways for Plant-Based Product Valorization

Rouzic¹,

Bruniaux²,

Raveschot³

et al. 2023

Lactobacillus - A Multifunctional Genus

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Today, plant production is increasing, but most industrial processes generate a lot of waste and by-products for which, in the current context, it is a priority to recycle or valorize them. One of the cheapest valorization routes is fermentation, in particular lactic fermentation by Lactobacillus species, which produces lactic acid and other molecules of industrial interest such as bioactive compounds such as anthocyanin, organic acid, peptides, or phenol, which are widely found in the plant matrix, mainly in cereals, grass, fruits, and vegetables. Bioactive compounds may exert beneficial health effects, such as antioxidant, anti-inflammatory, antimicrobial, or prebiotic activities. In addition, lactic acid fermentation can improve existing products and lead to new applications in food, livestock feeding and biotechnology, such as the production of lactic acid, protein, or silage. This chapter reviews the use of Lactobacillus strains in the fermentation process of many plant bioresources or by-products through their different bioactivities, active molecules, and applications.

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“…Uzunsoy / Uluslararası Tarım Araştırmalarında Yenilikçi Yaklaşımlar Dergisi / International Journal of Innovative Approaches in Agricultural Research, 2022, Vol. 6 (4) Kähkönen, Hopia & Heinonen, 2001;Wu, Gu, Prior & McKay, 2004;Osmiańzski & Wojdylo, 2005;Jakobek, Šeruga & Krivak, 2011;Kapci, 2013;Tolić et al, 2015;Szopa et al 2017;Dąbrowska et al, 2019;Denev, Číž, Kratchanova & Blazheva, 2019;Rudic et al, 2022Brӓunlich et al, 2013Denev et al, 2019;Daoutidou, Plessas, Alexopoulos & Mantzourani, 2021;Deng et al, 2021Ohgami et al, 2005Martin et al, 2014;Kang et al, 2017;Lee et al, 2018;Jang, Lee, Choi & Yim, 2020;Ali et al, 2021;Li et al, 2022Bhaswant, Shafie, Mathai, Mouatt & Brown, 2017Jeon et al, 2018;Jeong & Kim, 2019;Chen, Zhu & Meng, 2020;Mu et al, 2020Xie et al, 2017Kim et al, 2018;Jeong & Kim, 2019;Tasic et al, 2021Bell & Gochenaur, 2006Naruszewicz, Laniewska, Millo & Dłuzniewski, 2007;Olas et al, 2008;Ćujić et al, 2018;Istas et al, 2019Malik et al, 2003Lala et al, 2006;Kędzierska et al, 2013;Choi et al, 2018a;Thi & Hwang, 2018;…”

Section: Phenolic Content Of Aronia and Related Antioxidant Activitymentioning

confidence: 99%

“…Fermented aronia fruit extract fortified with γ-aminobutyric acid (GABA) including bioactive compounds exerted anti-inflammatory effects on female BALB/c mice (Ali et al, 2021). C3G was extracted from aronia and applied to mice which were exposed to PM10 (particules below 10 µM) for the investigation of anti-inflammatory activity (Cui et al, 2021).…”

Section: Antibacterial and Anti-inflammatory Activities Of Aroniamentioning

confidence: 99%

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Bioactivity of Aronia Products, and The Promising Use of Aronia in Dairy Industry

Uzunsoy¹

2022

IJIAAR

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In recent years, foods are not only valued in terms of taste and nutritional value, but also preferred in terms of post-consumption health effects. In particular, the trend towards functional foods that offer health benefits beyond their nutritional value to consumers has increased. Dairy products have a very important place among functional foods, and fruit-added dairy products like fermented milk, kefir and yogurt etc. lead the way due to the consumption preferences. Aronia (Aronia melanocarpa) is a forest fruit originating from North America, but it is grown in many parts of Europe lately. The fruit is very rich in proanthocyanins, anthocyanins, procyanidin, phenolic acids, flavonols and flavanones. It is known as the fruit with the highest antioxidant activity, and shows antidiabetic, anti-inflammatory, antiviral, antibacterial, hypotensive, cardioprotective, hepatoprotective and anticarcinogenic properties. The fruit is mostly used as ingredient in wine, marmalade, fruit juice, tea, extract and as dietary supplement or food colorant. The health-promoting effects of aronia due to its bioactivity are enhanced with the functional dairy products, and lately aronia-added yogurt and kefir are industrially manufactured and marketed for dairy consumers. This review focuses on the health effects of aronia and the promising use of the fruit in dairy products.

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Aronia melanocarpa Extract Fermented by Lactobacillus plantarum EJ2014 Modulates Immune Response in Mice

Cited by 9 publications

References 73 publications

Mucosal immunization with Lactiplantibacillus plantarum-displaying recombinant SARS-CoV-2 epitopes on the surface induces humoral and mucosal immune responses in mice

Mucosal immunization with Lactiplantibacillus plantarum-displaying recombinant SARS-CoV-2 epitopes on the surface induces humoral and mucosal immune responses in mice

Lactobacillus Use for Plant Fermentation: New Ways for Plant-Based Product Valorization

Bioactivity of Aronia Products, and The Promising Use of Aronia in Dairy Industry

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