Aromatic short peptide architectonics: Assembly and engineering

Tao, Kai; Wu, Haoran; Adler-Abramovich, Lihi; Zhang, Jiahao; Fan, Xinyuan; Wang, Yunxiao; Zhang, Yan; Tofail, Syed A.M.; Mei, Deqing; Li, Junbai; Gazit, Ehud

doi:10.1016/j.pmatsci.2024.101240

Cited by 3 publications

(2 citation statements)

References 257 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One notable category of supramolecular low molecular weight gels is based on N -functionalized dipeptides. , These gels, characterized by their biocompatibility and ease of synthesis, find utility in drug delivery, tissue engineering, sensing, and catalysis. , These gels can be formed using a range of triggers such as temperature, UV light, pH changes, solvent switches, and metal ions, , with the latter representing a useful class of cross-linkers. , …”

Section: Introductionmentioning

confidence: 99%

Metal Cross-Linked Supramolecular Gel Noodles: Structural Insights and Antibacterial Assessment

Ghosh,

Coulter,

Laverty

et al. 2024

Biomacromolecules

View full text Add to dashboard Cite

Achieving precise control over gelator alignment and morphology is crucial for crafting tailored materials and supramolecular structures with distinct properties. We successfully aligned the self-assembled micelles formed by a functionalized dipeptide 2NapFF into long 1-D "gel noodles" by cross-linking with divalent metal chlorides. We identify the most effective crosslinker for alignment, enhancing mechanical stability, and imparting functional properties. Our study shows that Group 2 metal ions are particularly suited for creating mechanically robust yet flexible gel noodles because of their ionic and nondirectional bonding with carboxylate groups. In contrast, the covalent nature and high directional bonds of d-block metal ions with carboxylates tend to disrupt the self-assembly of 2NapFF. Furthermore, the 2NapFF-Cu noodles demonstrated selective antibacterial activity, indicating that the potent antibacterial property of the copper(II) ion is preserved within the cross-linked system. By merging insights into molecular alignment, gel extrusion processing, and integrating specific functionalities, we illustrate how the versatility of dipeptide-based gels can be utilized in creating next-generation soft materials.

show abstract

Section: Introductionmentioning

confidence: 99%

Metal Cross-Linked Supramolecular Gel Noodles: Structural Insights and Antibacterial Assessment

Ghosh,

Coulter,

Laverty

et al. 2024

Biomacromolecules

View full text Add to dashboard Cite

show abstract

“…We have previously combined this LMWG with pH-responsive DBS-COOH; however, the p K a of DBS-COOH is 5.4, and assembly only occurs below this pH value, which is not ideal for biological applications. With the long-term goal of raising the stability of the second network in biological media, a literature survey identified peptide-based gelator Nap-FF (Figure A), which has a p K a value of 6.8, as a potentially interesting LMWG to combine with DBS-CONHNH 2 . , The p K a of Nap-FF is higher than the typical C-terminus of a peptide due to hydrophobically driven self-assembly. , Peptide gelators of this type are well understood through the careful work of a number of researchers. − In particular, Adams and co-workers have characterized multicomponent systems, gaining understanding across multiple length scales. − However, a combination of this peptide LMWG with other gelators having very different chemical structures remains rare. We also noted that the assembly of this class of peptide gel could be triggered by calcium ions, − not possible for our own acid-functionalized LMWG (DBS-COOH) and realized that this opened new possibilities in terms of gel patterning that might be relevant in biological systems.…”

Section: Introductionmentioning

confidence: 99%

Self-Sorting in Multicomponent Supramolecular Gels Using Different Assembly Triggers and Calcium Ion-Induced Diffusion Patterning

Tangsombun,

Smith

2024

Chem. Mater.

View full text Add to dashboard Cite

This paper investigates self-sorting and triggered assembly of multicomponent gels that combine sorbitol-based low-molecular-weight gelator (LMWG) DBS-CONHNH2 and peptide-based LMWG Nap-FF, with the assembly of Nap-FF being triggered either using glucono-δ-lactone (GdL) to lower pH or CaCl2. Changing triggers alters the way Nap-FF assembles on the molecular scale either as its acid form or its calcium salt, leading to different nanoscale networks and rheological behaviors. The choice of trigger impacts the properties of the two-component gels formed with DBS-CONHNH2. Using either trigger, the LMWGs self-sort on the molecular level into their own distinct assemblies. However, when using a GdL trigger, the sheet-like Nap-FF assemblies encourage DBS-CONHNH2 assembly, with the two assemblies interacting with one another on a network level as a result of interactions between the acylhydrazide and the carboxylic acid. Conversely, when using a CaCl2 trigger, the two fibrillar assemblies are independent of one another on the network level, with the carboxylic acid being bound to calcium in the form of its carboxylate salt, and unable to interact with DBS-CONHNH2. As such, GdL-triggering leads to molecular-level self-sorting and network-level coassembly, while CaCl2-triggering leads to both molecular-level and network-level self-sorting. Injecting the CaCl2 trigger into a preformed DBS-CONHNH2 gel, followed by its diffusion, creates dynamic, evolving, spatially-resolved self-sorted multicomponent gels, with stiffnesses differing by 2 orders of magnitude in different domains. Given the mild, biocompatible nature of CaCl2, it is suggested that this calcium ion diffusion approach to spatiotemporally resolved patterning of multidomain gels may have future relevance in cell-based studies.

show abstract

Peptide-Based Biomimetic Condensates via Liquid–Liquid Phase Separation as Biomedical Delivery Vehicles

Song,

Ivanov,

Yuan

et al. 2024

Biomacromolecules

View full text Add to dashboard Cite

Biomolecular condensates are dynamic liquid droplets through intracellular liquid−liquid phase separation that function as membraneless organelles, which are highly involved in various complex cellular processes and functions. Artificial analogs formed via similar pathways that can be integrated with biological complexity and advanced functions have received tremendous research interest in the field of synthetic biology. The coacervate droplet-based compartments can partition and concentrate a wide range of solutes, which are regarded as attractive candidates for mimicking phase-separation behaviors and biophysical features of biomolecular condensates. The use of peptide-based materials as phase-separating components has advantages such as the diversity of amino acid residues and customized sequence design, which allows for programming their phase-separation behaviors and the physicochemical properties of the resulting compartments. In this Perspective, we highlight the recent advancements in the design and construction of biomimicry condensates from synthetic peptides relevant to intracellular phase-separating protein, with specific reference to their molecular design, self-assembly via phase separation, and biorelated applications, to envisage the use of peptidebased droplets as emerging biomedical delivery vehicles.

show abstract

Aromatic short peptide architectonics: Assembly and engineering

Cited by 3 publications

References 257 publications

Metal Cross-Linked Supramolecular Gel Noodles: Structural Insights and Antibacterial Assessment

Metal Cross-Linked Supramolecular Gel Noodles: Structural Insights and Antibacterial Assessment

Self-Sorting in Multicomponent Supramolecular Gels Using Different Assembly Triggers and Calcium Ion-Induced Diffusion Patterning

Peptide-Based Biomimetic Condensates via Liquid–Liquid Phase Separation as Biomedical Delivery Vehicles

Contact Info

Product

Resources

About