Armillaria in the Northern Rockies : delineation of isolates into clones /

McDonald, Geral I.; Martin, N. E.

doi:10.5962/bhl.title.68857

Cited by 9 publications

(4 citation statements)

References 12 publications

(31 reference statements)

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“…Following the isolations of Armillaria in 1987, somatic incompatibility pairing tests among all isolates were used to identify individual genets that comprised the isolates, and isolates that fused in culture to form homogeneous colonies were considered as belonging to the same genet (e.g., [22][23][24]) ( Table 1). The somatic pairing of each genet was used to separate the genets at the species level [22,23,[25][26][27][28][29][30][31][32][33][34]. The initial species identification was performed by the somatic pairing (e.g., [28,29,32,35]) of each genet 2-16 times against well-characterized reference isolates of A. solidipes (15-32 reference isolates), A. sinapina Bérubé and Dessur (two reference isolates), and A. altimontana (8-20 reference isolates considered as NABS X when the pairing tests were performed), which were available at the time.…”

Section: Armillaria Genet and Species Identificationmentioning

confidence: 99%

Armillaria altimontana Is Associated with Healthy Western White Pine (Pinus monticola): Potential in Situ Biological Control of the Armillaria Root Disease Pathogen, A. solidipes

Warwell

McDonald

Hanna

et al. 2019

Forests

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Research Highlights: Two genets of Armillaria altimontana Brazee, B. Ortiz, Banik, and D.L. Lindner and five genets of Armillaria solidipes Peck (as A. ostoyae [Romagnesi] Herink) were identified and spatially mapped within a 16-year-old western white pine (Pinus monticola Doug.) plantation, which demonstrated distinct spatial distribution and interspecific associations. Background and Objectives: A. solidipes and A. altimontana frequently co-occur within inland western regions of the contiguous USA. While A. solidipes is well-known as a virulent primary pathogen that causes root disease on diverse conifers, little has been documented on the impact of A. altimontana or its interaction with A. solidipes on growth, survival, and the Armillaria root disease of conifers. Materials and Methods: In 1971, a provenance planting of P. monticola spanning 0.8 ha was established at the Priest River Experimental Forest in northern Idaho, USA. In 1987, 2076 living or recently dead trees were measured and surveyed for Armillaria spp. to describe the demography and to assess the potential influences of Armillaria spp. on growth, survival, and the Armillaria root disease among the study trees. Results: Among the study trees, 54.9% were associated with Armillaria spp. The genets of A. altimontana and A. solidipes comprised 82.7% and 17.3% of the sampled isolates (n = 1221) from the study plot, respectively. The mapped distributions showed a wide, often noncontiguous, spatial span of individual Armillaria genets. Furthermore, A. solidipes was found to be uncommon in areas dominated by A. altimontana. The trees colonized by A. solidipes were associated with a lower tree growth/survival and a substantially higher incidence of root disease than trees colonized only by A. altimontana or trees with no colonization by Armillaria spp. Conclusions: The results demonstrate that A. altimontana was not harmful to P. monticola within the northern Idaho planting. In addition, the on-site, species-distribution patterns suggest that A. altimontana acts as a long-term, in situ biological control of A. solidipes. The interactions between these two Armillaria species appear critical to understanding the Armillaria root disease in this region.

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Section: Armillaria Genet and Species Identificationmentioning

confidence: 99%

Armillaria altimontana Is Associated with Healthy Western White Pine (Pinus monticola): Potential in Situ Biological Control of the Armillaria Root Disease Pathogen, A. solidipes

Warwell

McDonald

Hanna

et al. 2019

Forests

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“…Somatic incompatibility pairing tests had previously been used to differentiate isolates into unique genets (McDonald and Martin 1988;Guillaumin et al 1991;Wu et al 1996), which represent a single genotype (Guillaumin et al 1996;Worrall 1997;Dettman and van der Kamp 2001). These genets were further identified to species using haploid · haploid mating, haploid · diploid pairing tests, diploid · diploid paring tests (Korhonen 1978;McDonald and Martin 1988;Mallett et al 1989) and/or restriction fragment length polymorphic (RFLP) analysis of the IGS-1 region of rDNA (Harrington and Wingfield 1995;White et al 1998;Kim et al 2000). Species identification was verified by sequence similarity within GenBank.…”

Section: Genet Selectionmentioning

confidence: 99%

“…Representative genets of A. ostoyae from the western USA (Oregon, Washington, Idaho, Montana, Utah and New Mexico; Table 1) and several genets as geographic outgroups (Russia, Finland, eastern USA and Mexico; Table 1) and other Armillaria species from the Northern Hemisphere (Table 2) were obtained from an archived collection at the USDA Forest Service, Rocky Mountain Research Station, Forestry Sciences Laboratory (Moscow, ID, USA). Somatic incompatibility pairing tests had previously been used to differentiate isolates into unique genets (McDonald and Martin 1988;Guillaumin et al 1991;Wu et al 1996), which represent a single genotype (Guillaumin et al 1996;Worrall 1997;Dettman and van der Kamp 2001). These genets were further identified to species using haploid · haploid mating, haploid · diploid pairing tests, diploid · diploid paring tests (Korhonen 1978;McDonald and Martin 1988;Mallett et al 1989) and/or restriction fragment length polymorphic (RFLP) analysis of the IGS-1 region of rDNA (Harrington and Wingfield 1995;White et al 1998;Kim et al 2000).…”

Section: Genet Selectionmentioning

confidence: 99%

Phylogeographic patterns of Armillariaostoyae in the western United States

et al. 2007

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Nuclear ribosomal DNA regions (i.e. large subunit, internal transcribed spacer, 5.8S and intergenic spacer) were sequenced using a direct-polymerase chain reaction method from Armillaria ostoyae genets collected from the western USA. Many of the A. ostoyae genets contained heterogeneity among rDNA repeats, indicating intragenomic variation and likely intraspecific hybridization. Intragenomic variation was verified by visually editing base-sequence offsets in regions with insertions/deletions, and using sequence-specific internal primers to resequence heterogeneous regions. Phylogenetic analyses with Bayesian Inference methods were used to define groups within A. ostoyae. Analysis of A. ostoyae from outside the western USA indicated the presence of a Circumboreal group of A. ostoyae that also occurs in Utah; two other phylogeographic groups were associated with the Rocky Mountain and Pacific Northwest regions of the USA. Mixed sequence types, an indication of intraspecific hybrids, were common in some geographic regions. Hybridization events may have influenced species evolution, contributing to variation in pathogenicity and virulence. The occurrence of these groups and intraspecific hybrids also indicates that paleogeography and paleoclimate may have influenced the phylogeography of A. ostoyae. In addition, other Armillaria species were examined for evolutionary relationships with the groups of A. ostoyae. These findings will provide a basis for future research relating ecological function to genetic diversity within A. ostoyae.

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“…Identification of Armillaria species used two methods. The first (Korhonen 1978;Mallett et al 1989;McDonald and Martin 1988) used diploid-diploid pairings, and the second used restriction fragment length polyporphisms (RFLPs) of the intergenic spacer I (IGS-I) ribosomal DNA region (White et al 1998). Armillaria ostoyae and North American Biological Species X (NABS X) were considered the pathogenic Armillaria species most likely to occur in our study area (G. McDonald, USDA Forest Service, personal communication) and were used as identification standards.…”

Section: Species Identificationmentioning

confidence: 99%

Coarse-scale population structure of pathogenicArmillariaspecies in a mixed-conifer forest in the Blue Mountains of northeast Oregon

Ferguson¹,

Dreisbach²,

Parks³

et al. 2003

Can. J. For. Res.

195

124

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The coarse-scale population structure of pathogenic Armillaria (Fr.) Staude species was determined on approximately 16 100 ha of relatively dry, mixed-conifer forest in the Blue Mountains of northeast Oregon. Sampling of recently dead or live, symptomatic conifers produced 112 isolates of Armillaria from six tree species. Armillaria species identifications done by using a polymerase chain reaction based diagnostic and diploiddiploid pairings produced identical results: 108 of the isolates were Armillaria ostoyae (Romagn.) Herink and four were North American Biological Species X (NABS X). Five genets of A. ostoyae and one of NABS X were identified through the use of somatic incompatibility pairings among the putatively diploid isolates. Armillaria ostoyae genet sizes were approximately 20, 95, 195, 260, and 965 ha; cumulative colonization of the study area was at least 9.5%. The maximum distance between isolates from the 965-ha A. ostoyae genet was approximately 3810 m, and use of three estimates of A. ostoyae spread rate in conifer forests resulted in age estimates for the genet ranging from 1900 to 8650 years. Results are discussed in relation to possible mechanisms that influenced the establishment, expansion, and expression of these genets; the genetic structure and stability of Armillaria; and the implications for disease management in this and similar forests.

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Armillaria in the Northern Rockies : delineation of isolates into clones /

Cited by 9 publications

References 12 publications

Armillaria altimontana Is Associated with Healthy Western White Pine (Pinus monticola): Potential in Situ Biological Control of the Armillaria Root Disease Pathogen, A. solidipes

Armillaria altimontana Is Associated with Healthy Western White Pine (Pinus monticola): Potential in Situ Biological Control of the Armillaria Root Disease Pathogen, A. solidipes

Phylogeographic patterns of Armillariaostoyae in the western United States

Coarse-scale population structure of pathogenicArmillariaspecies in a mixed-conifer forest in the Blue Mountains of northeast Oregon

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