Armigeres subalbatus is a potential vector for Zika virus but not dengue virus

Yang, Wenqiang; Zhao, Siyu; Xie, Yugu; Liu, Tong; Kong, Ling; Guo, Yijia; Xie, Zenghong; Liu, Peiwen; Liu, Tong

doi:10.1186/s40249-022-00990-0

Cited by 9 publications

(6 citation statements)

References 36 publications

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“…This species was also present across the different sites independently of the season. Armigeres subalbatus , which was present in almost all sites, is a potential vector of ZIKV [ 69 ] and is implicated in the transmission of filaria [ 70 ]. Aedes vexans, Cx.…”

Section: Discussionmentioning

confidence: 99%

Mosquito diversity (Diptera: Culicidae) and medical importance in four Cambodian forests

et al. 2023

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Background A total of 290 mosquito species are recorded in Cambodia among which 43 are known vectors of pathogens. As Cambodia is heavily affected by deforestation, a potential change in the dynamic of vector-borne diseases (VDBs) could occur through alteration of the diversity and density of sylvatic vector mosquitoes and induce an increase in their interactions with humans. Understanding mosquito diversity is therefore critical, providing valuable data for risk assessments concerning the (re)emergence of local VBDs. Consequently, this study mainly aimed to understand the spatial and temporal distribution of sylvatic mosquito populations of Cambodia by determining which factors impact on their relative abundance and presence. Methods A study was conducted in 12 sites from four forests in Cambodia. All mosquitoes, collected during the dry and rainy seasons, were morphologically identified. The diversity and relative density of mosquito species in each site were calculated along with the influence of meteorological and geographical factors using a quasi-Poisson generalized linear model. Results A total of 9392 mosquitoes were collected belonging to 13 genera and 85 species. The most represented genera were Culex, accounting for 46% of collected mosquitoes, and Aedes (42%). Besides being the most abundant species, Culex pseudovishnui and Aedes albopictus, which are known vectors of numerous arboviruses, were present in all sites during both dry and rainy seasons. The presence of mosquito species reported to be zoo-anthropophilic feeders was also observed in both forested and urban areas. Finally, this study demonstrated that altitude, temperature and precipitation impacted the abundance of mosquitoes but also influenced species community composition. Conclusion The results indicate an important diversity of mosquitoes in the four forests and an influence of meteorological and geographical factors on their community. Additionally, this work highlights in parallel the abundance of species considered to be of medical importance and therefore underlines the high risk of pathogen emergence/re-emergence in the region. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

Mosquito diversity (Diptera: Culicidae) and medical importance in four Cambodian forests

et al. 2023

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“…A study of vector competence for ZIKV in China indicated that A. aegypti had the highest transmissibility, followed by A. albopictus , whereas C. quinquefasciatus had no transmission ability ( 43 ). Another study conducted in China indicated that A. subalbatus was a potential vector for ZIKV ( 44 ). All the above-mentioned studies suggested that there might be restricted autochthonous ZIKV transmission in Southern China, but further research was needed.…”

Section: Discussionmentioning

confidence: 99%

The epidemiology of Aedes-borne arboviral diseases in Zhejiang, Southeast China: a 20 years population-based surveillance study

Ren,

Chen,

Ling

et al. 2023

Front. Public Health

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ObjectiveAedes-borne arboviral diseases were important public health problems in Zhejiang before the coronavirus disease 2019 (COVID-19) pandemic. This study was conducted to investigate the characteristics and change of the epidemiology of Aedes-borne arboviral diseases in the province.MethodsDescriptive analyses were conducted to summarize the epidemiology of Aedes-borne arboviral diseases during 2003–2022.ResultsA total of 3,125 cases, including 1,968 indigenous cases, were reported during 2003–2022. Approximately three-quarters of imported cases were infected from Southeast Asia. The number of annual imported cases increased during 2013–2019 (R2 = 0.801, p = 0.004) and peaked in 2019. When compared with 2003–2012, all prefecture-level cities witnessed an increase in the annual mean incidence of imported cases in 2013–2019 (0.11–0.42 per 100,000 population vs. 0–0.05 per 100,000 population) but a drastic decrease during 2020–2022 (0–0.03 per 100,000 population). The change in geographical distribution was similar, with 33/91 counties during 2003–2012, 86/91 during 2013–2019, and 14/91 during 2020–2022. The annual mean incidence of indigenous cases in 2013–2019 was 7.79 times that in 2003–2012 (0.44 vs. 0.06 per 100,000 population). No indigenous cases were reported between 2020–2022. Geographical extension of indigenous cases was also noted before 2020—from two counties during 2003–2012 to 44 during 2013–2019.ConclusionDengue, chikungunya fever, zika disease, and yellow fever are not endemic in Zhejiang but will be important public health problems for the province in the post-COVID-19 era.

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“…Vector susceptibility of Ae. albopictus to DENV-2 was evaluated by calculating the midgut infection rate (MIR; the number of infected midguts/the number of tested midguts), dissemination rate (DR; the number of infected ovaries/the number of infected midguts), and salivary gland infection rate (SGIR; the number of infected salivary glands/the number of tested mosquitoes) [ 31 , 32 ]. Genome copies of AaeDV were quantified in all midguts, ovaries, and salivary gland and genome copies were further determined in DENV2-positive tissues by absolute qRT-PCR.…”

Section: Methodsmentioning

confidence: 99%

Mosquito densovirus significantly reduces the vector susceptibility to dengue virus serotype 2 in Aedes albopictus mosquitoes (Diptera: Culicidae)

Kong

Xiao

et al. 2023

Infect Dis Poverty

Self Cite

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Background Dengue virus (DENV) is a major public health threat, with Aedes albopictus being the confirmed vector responsible for dengue epidemics in Guangzhou, China. Mosquito densoviruses (MDVs) are pathogenic mosquito-specific viruses, and a novel MDV was previously isolated from Ae. albopictus in Guangzhou. This study aims to determine the prevalence of MDVs in wild Ae. albopictus populations and investigate their potential interactions with DENV and impact on vector susceptibility for DENV. Methods The prevalence of MDV in wild mosquitoes in China was investigated using open access sequencing data and PCR detection in Ae. albopictus in Guangzhou. The viral infection rate and titers in MDV-persistent C6/36 cells were evaluated at 12, 24, 48, 72, 96, and 120 h post infection (hpi) by indirect immunofluorescence assay (IFA) and real time quantitative PCR (RT-qPCR). The midgut infection rate (MIR), dissemination rate (DR), and salivary gland infection rate (SGIR) in various tissues of MDV-infected mosquitoes were detected and quantified at 0, 5, 10, and 15 days post infection (dpi) by RT-PCR and RT-qPCR. The chi-square test evaluated dengue virus serotype 2 (DENV-2) and Aedes aegypti densovirus (AaeDV) infection rates and related indices in mosquitoes, while Tukey's LSD and t-tests compared viral titers in C6/36 cells and tissues over time. Results The results revealed a relatively wide distribution of MDVs in Aedes, Culex, and Anopheles mosquitoes in China and an over 68% positive rate. In vitro, significant reductions in DENV-2 titers in supernatant at 120 hpi, and an apparent decrease in DENV-2-positive cells at 96 and 120 hpi were observed. In vivo, DENV-2 in the ovaries and salivary glands was first detected at 10 dpi in both monoinfected and superinfected Ae. albopictus females, while MDV superinfection with DENV-2 suppressed the salivary gland infection rate at 15 dpi. DENV-2 titer in the ovary and salivary glands of Ae. albopictus was reduced in superinfected mosquitoes at 15 dpi. Conclusions MDVs is widespread in natural mosquito populations, and replication of DENV-2 is suppressed in MDV-infected Ae. albopictus, thus reducing vector susceptibility to DENV-2. Our study supports the hypothesis that MDVs may contribute to reducing transmission of DENV and provides an alternative strategy for mosquito-transmitted disease control. Graphical abstract

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Armigeres subalbatus is a potential vector for Zika virus but not dengue virus

Cited by 9 publications

References 36 publications

Mosquito diversity (Diptera: Culicidae) and medical importance in four Cambodian forests

Mosquito diversity (Diptera: Culicidae) and medical importance in four Cambodian forests

The epidemiology of Aedes-borne arboviral diseases in Zhejiang, Southeast China: a 20 years population-based surveillance study

Mosquito densovirus significantly reduces the vector susceptibility to dengue virus serotype 2 in Aedes albopictus mosquitoes (Diptera: Culicidae)

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