Armc8 is an evolutionarily conserved armadillo protein involved in cell–cell adhesion complexes through multiple molecular interactions

Gul, Ismail Sahin; Hulpiau, Paco; Sanders, Ellen; Roy, Frans van; Hengel, Jolanda van

doi:10.1042/bsr20180604

Cited by 8 publications

(7 citation statements)

References 40 publications

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“…For example, NMNAT3 maintains cell differentiation by maintaining mitochondrial content (Son et al, 2016 ; Yu et al, 2020 ). ARMC8 is involved in the adherence of regulatory cells to cells and is associated with cell differentiation in ovarian cancer tumors (Jiang et al, 2015 ; Gul et al, 2019 ). SOX14 is associated with apoptosis in cancer cells in the sexual reproductive system (Stanisavljevic et al, 2017 ) and is a crucial determinant of allergy development in Drosophila (Ritter and Beckstead, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of a Goat Intersexuality-Associated Novel Variant Through Genome-Wide Resequencing and Hi-C

et al. 2021

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Background: Polled intersex syndrome (PIS) leads to reproductive disorders in goats and exerts a heavy influence on goat breeding. Since 2001, the core variant of an 11.7 kb deletion at ~129 Mb on chromosome 1 (CHI1) has been widely used as a genetic diagnostic criterion. In 2020, a ~0.48 Mb insertion within the PIS deletion was identified by sequencing in XX intersex goats. However, the suitability of this variation for the diagnosis of intersex goats worldwide and its further molecular genetic mechanism need to be clarified.Results: The whole-genome selective sweep of intersex goats from China was performed with whole-genome next-generation sequencing technology for large sample populations and a case–control study on interbreeds. A series of candidate genes related to the goat intersexuality phenotype were found. We further confirmed that a ~0.48 Mb duplicated fragment (including ERG and KCNJ15) downstream of the ~20 Mb PIS region was reversely inserted into the PIS locus in intersex Chinese goats and was consistent with that in European Saanen and Valais black-necked goats. High-throughput chromosome conformation capture (Hi-C) technology was then used to compare the 3D structures of the PIS variant neighborhood in CHI1 between intersex and non-intersex goats. A newly found structure was validated as an intrachromosomal rearrangement. This inserted duplication changed the original spatial structure of goat CHI1 and caused the appearance of several specific loop structures in the adjacent ~20 kb downstream region of FOXL2.Conclusions: Results suggested that the novel complex PIS variant genome was sufficient as a broad-spectrum clinical diagnostic marker of XX intersexuality in goats from Europe and China. A series of private dense loop structures caused by segment insertion into the PIS deletion might affect the expression of FOXL2 or other neighboring novel candidate genes. However, these structures require further in-depth molecular biological experimental verification. In general, this study provided new insights for future research on the molecular genetic mechanism underlying female-to-male sex reversal in goats.

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Section: Discussionmentioning

confidence: 99%

Identification of a Goat Intersexuality-Associated Novel Variant Through Genome-Wide Resequencing and Hi-C

et al. 2021

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“…Armadillo-repeat-containing protein 8 belongs to the family of armadillo-repeat-containing proteins, which have been found to be involved in diverse cellular functions including cell–cell contacts and intracellular signaling. 30 Herein, we assessed the possible effect of ARMC8 in UVB-induced damage on HaCaT cells. After UVB irradiation, ARMC8 mRNA and protein levels were significantly reduced, suggesting the potential role of ARMC8 in UVB-mediated HaCaT cell damage.…”

Section: Discussionmentioning

confidence: 99%

MiR-664 Protects Against UVB Radiation-Induced HaCaT Cell Damage via Downregulating ARMC8

et al. 2020

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Background: MiR-664 has been demonstrated to play an important role in dermal diseases. However, the functions of miR-664 in ultraviolet B (UVB) radiation-induced keratinocytes damage remain to be elucidated. Objective: The present study aimed to investigate the molecular mechanisms under the UVB-induced keratinocytes damage and provide translational insights for future therapeutics and UVB protection. Methods: HaCaT cells were transfected with miR-664, either alone or combined with UVB irradiation. Levels of messenger RNA and protein were tested by quantitative real-time polymerase chain reaction and Western blot analyses. Cell proliferation, percentage of apoptotic cells, and expression levels of apoptosis-related factors were measured by Cell Counting Kit-8 assay, flow cytometry assay, and Western blot analysis, respectively. Results: We found that a significant increase in miR-664 was observed in UVB-induced HaCaT cells. Overexpressed miR-664 promoted cell vitalities and suppressed apoptosis of UVB-induced HaCaT cells. Additionally, the loss/gain of armadillo-repeat-containing protein 8 (ARMC8) rescued/blocked the effects of miR-664 on the proliferation of UVB-induced HaCaT cells. Conclusions: Our data demonstrate that miR-664 functions as a protective regulator in UVB-induced HaCaT cells via regulating ARMC8.

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“…Almost all studies on the ARMC8 functions in promoting tumorigenesis and metastasis were based on these two mechanisms. For example, regarding cell adhesion and tumor metastasis, ARMC8 upregulates matrix metalloproteinase-7 and Snail and degrades α-catenin to disrupt the α-catenin/E-cadherin complex and promote the migration and invasion of hepatocellular carcinoma, breast cancer, lung adenocarcinoma, osteosarcoma, hepatocellular carcinoma, ovarian cancer, bladder cancer, colon cancer, and others ( Jiang et al, 2015a ; Jiang et al, 2015b ; Zhao et al, 2016 ; Gul et al, 2019 ). Concerning E-3 ligase activity and other related biological processes, ARMC8 is involved in the regulation of pyrimidine metabolism, the pentose phosphate pathway, and the cellular functions of integrin signaling by controlling the proliferation and differentiation of lung adenocarcinoma ( Amin et al, 2015 ); ARMC8 upregulates the expression of β-catenin, cyclin D1, MMP7, and c-Myc induced by TGF-β1 by activating the canonical Wnt signaling pathway and thereby promote the proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of lung cancer and bladder cancer cells ( Xie et al, 2014 ; Jiang et al, 2016 ; Liang et al, 2017 ).…”

Section: Functional Interactions Of the Armc Subfamily In Specific Diseasesmentioning

confidence: 99%

ARMC Subfamily: Structures, Functions, Evolutions, Interactions, and Diseases

Huang

Jiang

Gao

et al. 2021

Front. Mol. Biosci.

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Armadillo repeat-containing proteins (ARMCs) are widely distributed in eukaryotes and have important influences on cell adhesion, signal transduction, mitochondrial function regulation, tumorigenesis, and other processes. These proteins share a similar domain consisting of tandem repeats approximately 42 amino acids in length, and this domain constitutes a substantial platform for the binding between ARMCs and other proteins. An ARMC subfamily, including ARMC1∼10, ARMC12, and ARMCX1∼6, has received increasing attention. These proteins may have many terminal regions and play a critical role in various diseases. On the one hand, based on their similar central domain of tandem repeats, this ARMC subfamily may function similarly to other ARMCs. On the other hand, the unique domains on their terminals may cause these proteins to have different functions. Here, we focus on the ARMC subfamily (ARMC1∼10, ARMC12, and ARMCX1∼6), which is relatively conserved in vertebrates and highly conserved in mammals, particularly primates. We review the structures, biological functions, evolutions, interactions, and related diseases of the ARMC subfamily, which involve more than 30 diseases and 40 bypasses, including interactions and relationships between more than 100 proteins and signaling molecules. We look forward to obtaining a clearer understanding of the ARMC subfamily to facilitate further in-depth research and treatment of related diseases.

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Armc8 is an evolutionarily conserved armadillo protein involved in cell–cell adhesion complexes through multiple molecular interactions

Cited by 8 publications

References 40 publications

Identification of a Goat Intersexuality-Associated Novel Variant Through Genome-Wide Resequencing and Hi-C

Identification of a Goat Intersexuality-Associated Novel Variant Through Genome-Wide Resequencing and Hi-C

MiR-664 Protects Against UVB Radiation-Induced HaCaT Cell Damage via Downregulating ARMC8

ARMC Subfamily: Structures, Functions, Evolutions, Interactions, and Diseases

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