Armadillo repeat-containing protein 1 is a dual localization protein associated with mitochondrial intermembrane space bridging complex

Wagner, Fabienne; Kunz, T.; Chowdhury, Suvagata Roy; Thiede, Bernd; Fraunholz, Martin; Eger, Debora; Kozjak-Pavlovic, Vera

doi:10.1101/656934

Cited by 2 publications

(2 citation statements)

References 41 publications

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“…Volcano plot figures were generated in Python using the matplotlib package. Figures that specify submitochondrial localization are based on annotations from Mitocarta 3.0 (66), with the following modifications: subcellular localizations for TDRKH, HSDL1, and ARMC1 were added manually based on published data (67) (68), TMEM11 is annotated as IM in Mitocarta 3.0; however, a recent preprint provides evidence that TMEM11 is largely in the OM, so this gene was included in analysis of OM proteins in our proteomic data (69).…”

Section: Methodsmentioning

confidence: 99%

MTCH2 is a mitochondrial outer membrane protein insertase

Guna

Stevens

Inglis

et al. 2022

Preprint

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In the mitochondrial outer membrane, tail-anchored (TA) proteins play critical roles in cytoplasmic-mitochondrial communication. Using genome-wide CRISPRi screens, we identify factors involved in mitochondrial TA biogenesis in human cells. We show that MTCH2, and its paralog MTCH1, are required for insertion of biophysically diverse mitochondrial TAs, but not outer membrane b-barrel proteins. In a reconstituted system, purified MTCH2 is sufficient to mediate insertion into proteoliposomes. Functional and mutational studies reveal that MTCH2 uses membrane-embedded hydrophilic residues to function as a gatekeeper for outer membrane protein biogenesis, controlling mislocalization of TAs into the endoplasmic reticulum and the sensitivity of leukemia cells to apoptosis. Our identification of MTCH2 as an insertase provides a mechanistic explanation for the diverse phenotypes and disease states associated with MTCH2 dysfunction.

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Section: Methodsmentioning

confidence: 99%

MTCH2 is a mitochondrial outer membrane protein insertase

Guna

Stevens

Inglis

et al. 2022

Preprint

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“…Armadillo repeat-containing 1 (ARMC1) was originally found in mitochondrion as a structural subunit [12]. Its gene locates at position 8q13.1 of the human chromosome.…”

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confidence: 99%

The Valuable Role of Armc1 in Invasive Breast Cancer as a Novel Biomarker

Gan

Zhong

et al. 2022

Preprint

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Background: Invasive breast carcinoma (BRCA) is a common type of breast cancer with high incidence in clinics, so it is significant to find an effective biomarker for BRCA diagnosis and treatment. Although some Armadillo (Arm)-repeat proteins families are confirmed to be biomarkers in cancers, the role of Armadillo repeat-containing 1 (ARMC1) in BRCA remains unknown.Methods: We analyzed the ARMC1 expression in normal breast tissues and BRCA samples, and its association with overall survival by the public database. χ² test evaluated the risks associated with ARMC1 expression in TCGA-BRCA patient samples. The ARMC1 mutations in BRCA were explored in the cBioportal database. Besides, the GO and KEGG analysis was used to explore the potential signaling pathways of ARMC1 in BRCA. Lastly, Immunohistochemistry and immunohistochemistry were performed to validate the ARMC1 expression in BRCA.Results: ARMC1 level in tumor sample was significantly higher than that in normal tissue, and it was also related to lower survival. The factors in clinical patients such as tumor stage and grade and histology were associated with ARMC1 expression. There were 32% of ARMC1 genetic mutations in BRCA, and the amplification and high expression made up the majority of them. Also, ARMC1 might regulate BRCA by involving in the cell cycle. Increased ARMC1 expression was found in clinical breast carcinoma tissues by our confirmatory experiments.Conclusions: All the results revealed that ARMC1 may play a significant role in BRCA as a biomarker, it provides valuable clues for the treatment and diagnosis of invasive breast cancer.

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Armadillo repeat-containing protein 1 is a dual localization protein associated with mitochondrial intermembrane space bridging complex

Cited by 2 publications

References 41 publications

MTCH2 is a mitochondrial outer membrane protein insertase

MTCH2 is a mitochondrial outer membrane protein insertase

The Valuable Role of Armc1 in Invasive Breast Cancer as a Novel Biomarker

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