Aristolone in Nardostachys jatamansi DC. induces mesenteric vasodilation and ameliorates hypertension via activation of the KATP channel and PDK1-Akt-eNOS pathway

Fang, Jingmei; Li, Ran; Zhang, Yue; Oduro, Patrick Kwabena; Li, Sa; Leng, Ling; Wang, Zhimei; Rao, Yao; Niu, Lu; Wu, Hong‐Hua; Wang, Qilong

doi:10.1016/j.phymed.2022.154257

Cited by 4 publications

(5 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Resultsmentioning

confidence: 99%

“…Previous studies have revealed that nardosinone possesses significant anti-neuroinflammatory [ 10 ] and vasodilatory (IC 50 254.59 μM) effects [ 27 ]. In this study, these two activities of nardosinone and its primary degradation product (2–deoxokanshone M, 1 ) were evaluated based on our previously reported methods [ 26 , 27 ]. As shown in Figure 7 , nardosinone could significantly inhibit the inflammatory factor NO production of the LPS-induced neuroinflammation model in BV–2 microglia cells, which is consistent with previous results reported (IC 50 : 37.82–74.21 μM) [ 10 ] and compared with minocycline (IC 50 = 23.69 ± 2.01 μM) [ 28 ].…”

Section: Resultsmentioning

confidence: 99%

“…Given these results, it appears that the degradation mentioned above is beneficial for nardosinone in the treatment of vasodilation-related diseases, exemplified by hypertension [ 27 ], and is unfavorable for nardosinone in the aid of neuroinflammation-associated neurodegenerative disorders such as Alzheimer’s disease, parkinsonian syndromes, and amyotrophic lateral sclerosis [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

“…Using the method previously described [ 27 ], the intact endothelium aorta was used to measure the vasorelaxation activity of the isolated compounds.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Degradation Profiling of Nardosinone at High Temperature and in Simulated Gastric and Intestinal Fluids

Xue

Yang

et al. 2023

Molecules

Self Cite

View full text Add to dashboard Cite

Nardosinone, a predominant bioactive product from Nardostachys jatamansi DC, is well-known for its promising therapeutic applications, such as being used as a drug on anti-inflammatory, antidepressant, cardioprotective, anti-neuroinflammatory, anti-arrhythmic, anti-periodontitis, etc. However, its stability under varying environmental conditions and its degradation products remain unclear. In this study, four main degradation products, including two previously undescribed compounds [2–deoxokanshone M (64.23%) and 2–deoxokanshone L (1.10%)] and two known compounds [desoxo-narchinol A (2.17%) and isonardosinone (3.44%)], were firstly afforded from the refluxed products of nardosinone in boiling water; their structures were identified using an analysis of the extensive NMR and X–ray diffraction data and the simulation and comparison of electronic circular dichroism spectra. Compared with nardosinone, 2–deoxokanshone M exhibited potent vasodilatory activity without any of the significant anti-neuroinflammatory activity that nardosinone contains. Secondly, UPLC–PDA and UHPLC–DAD/Q–TOF MS analyses on the degradation patterns of nardosinone revealed that nardosinone degraded more easily under high temperatures and in simulated gastric fluid compared with the simulated intestinal fluid. A plausible degradation pathway of nardosinone was finally proposed using nardosinonediol as the initial intermediate and involved multiple chemical reactions, including peroxy ring-opening, keto–enol tautomerization, oxidation, isopropyl cleavage, and pinacol rearrangement. Our findings may supply certain guidance and scientific evidence for the quality control and reasonable application of nardosinone-related products.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…Using the method previously described [ 27 ], the intact endothelium aorta was used to measure the vasorelaxation activity of the isolated compounds.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Degradation Profiling of Nardosinone at High Temperature and in Simulated Gastric and Intestinal Fluids

Xue

Yang

et al. 2023

Molecules

Self Cite

View full text Add to dashboard Cite

show abstract

“…The 3D chemical structure of senkyunolide A, or scopoletin, was obtained from the PubChem database. The X-ray crystal protein preparation and the molecular docking were done using Discovery Studio Client (2019) and Autodock Vina, as described in our previous study ( Fang et al, 2022 ), and the results were visualized with PyMOL.…”

Section: Methodsmentioning

confidence: 99%

Suxiao Jiuxin Pill attenuates acute myocardial ischemia via regulation of coronary artery tone

Zhan

Wang

et al. 2023

Front. Pharmacol.

Self Cite

View full text Add to dashboard Cite

Suxiao Jiuxin Pill (SJP) is a well-known traditional Chinese medicine drug used to manage heart diseases. This study aimed at determining the pharmacological effects of SJP in acute myocardial infarction (AMI), and the molecular pathways its active compounds target to induce coronary artery vasorelaxation. Using the AMI rat model, SJP improved cardiac function and elevated ST segment. LC-MS and GC-MS detected twenty-eight non-volatile compounds and eleven volatile compounds in sera from SJP-treated rats. Network pharmacology analysis revealed eNOS and PTGS2 as the key drug targets. Indeed, SJP induced coronary artery relaxation via activation of the eNOS-NO pathway. Several of SJP’s main compounds, like senkyunolide A, scopoletin, and borneol, caused concentration-dependent coronary artery relaxation. Senkyunolide A and scopoletin increased eNOS and Akt phosphorylation in human umbilical vein endothelial cells (HUVECs). Molecular docking and surface plasmon resonance (SPR) revealed an interaction between senkynolide A/scopoletin and Akt. Vasodilation caused by senkyunolide A and scopoletin was inhibited by uprosertib (Akt inhibitor) and eNOS/sGC/PKG axis inhibitors. This suggests that senkyunolide A and scopoletin relax coronary arteries through the Akt-eNOS-NO pathway. In addition, borneol induced endothelium-independent vasorelaxation of the coronary artery. The Kv channel inhibitor 4-AP, KCa2+ inhibitor TEA, and Kir inhibitor BaCl2 significantly inhibited the vasorelaxant effect of borneol in the coronary artery. In conclusion, the results show that Suxiao Jiuxin Pill protects the heart against acute myocardial infarction.

show abstract

Vasodilatory constituents of essential oil from Nardostachys jatamansi DC.: Virtual screening, experimental validation and the potential molecular mechanisms

Xue

Liu

Oduro

et al. 2023

Arabian Journal of Chemistry

View full text Add to dashboard Cite

Aristolone in Nardostachys jatamansi DC. induces mesenteric vasodilation and ameliorates hypertension via activation of the KATP channel and PDK1-Akt-eNOS pathway

Cited by 4 publications

References 30 publications

Degradation Profiling of Nardosinone at High Temperature and in Simulated Gastric and Intestinal Fluids

Degradation Profiling of Nardosinone at High Temperature and in Simulated Gastric and Intestinal Fluids

Suxiao Jiuxin Pill attenuates acute myocardial ischemia via regulation of coronary artery tone

Vasodilatory constituents of essential oil from Nardostachys jatamansi DC.: Virtual screening, experimental validation and the potential molecular mechanisms

Contact Info

Product

Resources

About