Aristolochic Acids From Aristolochia Bridgesii, a Host-Plant of Battus Polydamas Archidamas

Abstract

The present study confirms the hypothesis that aristolochic acids are present in Aristolochia bridgesii. The chemical composition of aristolochic acids in this species is qualitatively similar to that of Aristolochia chilensis, with the two species differing only in the total amount of aristolochic acids present, and in the relative proportions of each aristolochic acid. These results are consistent with the fact that the two Aristolochia species in Chile both serve as host plants to Battus polydamas archidamas (Lepidoptera, Papilionidae, Troidinii).

Aristolochic Acids in the Roots of Aristolochia Chilensis, a Dangerous Chilean Medicinal Plant

“…25 The AAs identified in A. chilensis roots, expressed as mg/kg of dry material are shown in Table 1. Although the same set of compounds, with the exception of compound (8), were identified in the leaves and stems, 22 their concentrations in the roots are very different. Phenolic components and the 6-0-β-D-glucopyranoside of aristolochic acid-IVa (8) accounted for nearly 72.5% of the total mixture, whereas they represented only 27% of the total in the leaves and stems.…”

“…[14][15][16][17][18][19][20][21][22][23][24] Their UV spectra ( Figure Nº 1) and retention times were compared, and co-injection was performed using standards of AA-I (1), AA-II (2), AA-III (3), AA-IV (4), AA-Ia (5), AA-IIIa (6), and AA-IVa (7), which had been previously isolated from A. chilensis, A. argentina, and Battus polydamas archidamas 22,25,26 . Quantification was based on peak areas of chromatograms taken at 254 nm.…”

“…The clear filtrate was adjusted to pH 2 with HCl and extracted with AcOEt (6 x 10 mL). Evaporation of the combined extracts in vacuo yielded a purified fraction of phenolic AAs (45.5 mg) 22 . This procedure was repeated using five samples (40 g) of A.chilensis roots obtained from five different plants 22 .…”

“…Gradient elution was performed using a mobile phase of 0.1% acetic acid in water (solution A) and 0.1% acetic acid in acetonitrile (solution B) in the following manner: 0-5 min, isocratic elution with 70% A / 30% B; 5-45 min, linear gradient from 70% A / 30% B to 55% A / 45% B. A Waters 2996 diode-array-detector (DAD) was used for detection, and spectra were recorded at wavelengths between 200 and 800 nm 22,24 . The AA fractions from the five collected samples (40 g each), each of which was obtained from a different plant, were analyzed independently.…”

“…In this study, high-performance liquid chromatography with diodearray detection (HPLC-DAD), a technique widely used for the detection and quantification of AAs in herbal medicine [14][15][16][17][18][19][20][21][22][23][24] was used to determine the chemical composition of AAs from the roots of A. chilensis. The findings reveal that the roots contain a significant amount of AAs, making it dangerous to use A. chilensis as a medicinal plant because of the harmful effects of AAs.…”