ARHGAP1 Transported with Influenza Viral Genome Ensures Integrity of Viral Particle Surface through Efficient Budozone Formation

Kuroki, Takahiro; Hatta, Tomohisa; Natsume, Tohru; Sakai, Noriko; Yagi, Akira; Kato, Kohsuke; Nagata, Kyosuke; Kawaguchi, Atsushı

doi:10.1128/mbio.00721-22

Cited by 2 publications

(1 citation statement)

References 70 publications

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“…The cytoplasmic tail of HA possesses multiple basic residues which interact with phosphoinositides (e.g., PIP2), modulating protein clustering and membrane association [ 31 , 32 ]. Additionally, several studies have investigated the interplay between lipids and the IAV matrix protein 2 (M2) [ 30 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ]. It has been proposed that M2 is transported to the apical PM independently from HA and NA through its interaction with the phosphatidylserine (PS)-conjugated, microtubule-associated protein 1 light chain 3 protein (LC3-II) [ 37 , 41 , 42 , 43 ].…”

Section: Introductionmentioning

confidence: 99%

Influenza A Virus Infection Alters Lipid Packing and Surface Electrostatic Potential of the Host Plasma Membrane

Petrich,

Chiantia

2023

Viruses

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The pathogenesis of influenza A viruses (IAVs) is influenced by several factors, including IAV strain origin and reassortment, tissue tropism and host type. While such factors were mostly investigated in the context of virus entry, fusion and replication, little is known about the viral-induced changes to the host lipid membranes which might be relevant in the context of virion assembly. In this work, we applied several biophysical fluorescence microscope techniques (i.e., Förster energy resonance transfer, generalized polarization imaging and scanning fluorescence correlation spectroscopy) to quantify the effect of infection by two IAV strains of different origin on the plasma membrane (PM) of avian and human cell lines. We found that IAV infection affects the membrane charge of the inner leaflet of the PM. Moreover, we showed that IAV infection impacts lipid–lipid interactions by decreasing membrane fluidity and increasing lipid packing. Because of such alterations, diffusive dynamics of membrane-associated proteins are hindered. Taken together, our results indicate that the infection of avian and human cell lines with IAV strains of different origins had similar effects on the biophysical properties of the PM.

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Section: Introductionmentioning

confidence: 99%

Influenza A Virus Infection Alters Lipid Packing and Surface Electrostatic Potential of the Host Plasma Membrane

Petrich,

Chiantia

2023

Viruses

View full text Add to dashboard Cite

show abstract

Influenza A virus infection alters lipid packing and surface electrostatic potential of the host plasma membrane

Petrich

Chiantia

2023

Preprint

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The pathogenesis of influenza A viruses (IAVs) is influenced by several factors, including IAV strain origin and reassortment, tissue tropism and host type. While such factors were mostly investigated in the context of virus entry, fusion and replication, little is known about the viral-induced changes to the host lipid membranes which might be relevant in the context of virion assembly. In this work, we applied several biophysical fluorescence microscope techniques (i.e., Förster energy resonance transfer, generalized polarization imaging and scanning fluorescence correlation spectroscopy) to quantify the effect of infection by two IAV strains of different origin on the plasma membrane (PM) of avian and human cell lines. We found that IAV infection affects the membrane charge of the inner leaflet of the PM. Moreover, we showed that IAV infection impacts lipid-lipid interactions by decreasing membrane fluidity and increasing lipid packing. Because of such alterations, diffusive dynamics of membrane-associated proteins are hindered. Taken to-gether, our results indicate that the infection of avian and human cell lines with IAV strains of different origins had similar effects on the biophysical properties of the PM.

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ARHGAP1 Transported with Influenza Viral Genome Ensures Integrity of Viral Particle Surface through Efficient Budozone Formation

Abstract: The endocytic transport of the influenza viral genome triggers the clustering of viral membrane proteins at the plasma membrane to form the viral budozone. However, host factors that promote viral budozone formation in concert with viral genome transport have not been identified.

Cited by 2 publications

References 70 publications

Influenza A Virus Infection Alters Lipid Packing and Surface Electrostatic Potential of the Host Plasma Membrane

Influenza A Virus Infection Alters Lipid Packing and Surface Electrostatic Potential of the Host Plasma Membrane

Influenza A virus infection alters lipid packing and surface electrostatic potential of the host plasma membrane

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