Argyrophilic nucleolar organizing region associated protein synthesis in hair root cells of humans at different developmental stages and sex

Eröz, Recep; Yılmaz, Seçil; Cücer, Nurhan

doi:10.3109/10520295.2013.769632

Cited by 22 publications

(18 citation statements)

References 25 publications

(11 reference statements)

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“…Morphometric analysis of AgNOR proteins in interphase in cancer cells has been used to identify various benign and malignant lesions, to establish prognoses, and to determine the proliferative activity of cells (Cucer et al, ; Derenzini et al, ; Eroz et al, ). In recent studies, morphometric analysis of AgNOR protein quantity has been also used to evaluate cellular activity in other cell types such as hair root cells of human (Eroz et al, ).…”

Section: Introductionmentioning

confidence: 99%

Nuclear AgNOR protein enhancement in nucleoplasms of peripheral blood lymphocytes of babies/children with down syndrome

Imamoglu

Eröz

Canatan

et al. 2016

Microscopy Res & Technique

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Down syndrome (DS) is one of the most common chromosomal disorders. The factors contributing to the mental retardation together with other defects in this syndrome have not been fully explained. Individuals with DS have extra rRNA gene family since they carry an extra chromosome 21. The few reports available are on the relationship between the nucleolus organizer regions (NORs) and DS phenotype. The in vivo regulation of NORs expression on the extra chromosome 21 is not completely understood. Previous studies have shown that nucleoli of lymphocytes from infants (mostly neonates) with DS contain more in vivo and in vitro nucleolar AgNOR proteins when compared with healthy infants. The objective of this study is to compare the in vivo nuclear AgNOR protein level in nucleoplasms (also called as karyoplasm) of nonstimulated peripheral blood lymphocytes from babies/children with DS and healthy controls. Peripheral blood samples obtained from 20 babies/children with DS and 20 matched healthy controls were smeared on clean glass slides and then AgNOR staining was performed. The AgNOR protein level in nucleoplasms of lymphocytes from both groups was calculated using a computer program. Nearly 100 interphase nuclei per individual were analysed. Average nuclear AgNOR protein levels in nucleoplasms of lymphocytes from babies/children with DS were found to be significantly higher than those of the controls (P < 0.001). On the basis of our present results, we propose that the increase of nuclear AgNOR protein in in vivo conditions may contribute to the formation of DS phenotypes.

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Section: Introductionmentioning

confidence: 99%

Nuclear AgNOR protein enhancement in nucleoplasms of peripheral blood lymphocytes of babies/children with down syndrome

Imamoglu

Eröz

Canatan

et al. 2016

Microscopy Res & Technique

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“…Therefore, argyrophilic NOR (AgNOR)associated proteins are one of the most reliable methods used to visualize nucleoli in interphase nuclei. 12 Numerous studies have been performed concerning the importance of the interphase quantity of AgNOR in tumor pathology, 13,14 hair root cells of humans, 15,16 developmental stages of Down syndrome in infants, 17 and so on. However, to the best of our knowledge, there are no studies in the literature about the relationship between AgNOR proteins and CO exposure in lung cells.…”

Section: Introductionmentioning

confidence: 99%

Is one-time carbon monoxide intoxication harmless? Evaluation by argyrophilic nucleolar-organizing regions staining method

et al. 2014

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In carbon monoxide (CO) poisoning, CO affects the oxygen-carrying capacity of the hemoglobin molecule. Nucleolar-organizing regions (NORs) are genetic loci on chromosomes that are composed of ribosomal DNA and proteins. NORs can be stained with silver. A total of 18 rats were exposed to CO in three different concentrations (1000, 3000, and 5000 ppm) with 6 rats as controls. The animals were euthanized 7 days after CO intoxication. Lung tissues were taken, embedded in paraffin blocks, and sectioned at 5 μm thickness. Argyrophilic nucleolar-organizing region (AgNOR) staining was carried out. One hundred nuclei per individual were evaluated, and total AgNOR number per total nuclear number and total AgNOR area per nuclear area (TAA/NA) for each nucleus were analyzed. The CO exposure groups had significantly higher TAA/NA values and AgNOR numbers than the control group ( p < 0.05). Although the differences between 1000 ppm and the other two CO-exposed groups were meaningful ( p < 0.05) in the TAA/NA values, there were no differences among the CO exposure groups for the AgNOR number ( p > 0.05). The increase in TAA/NA value depends on the increase in the CO exposure. Significant correlations between both the AgNOR values and histopathological scoring methods were found. Therefore, AgNOR staining method may be used as an indirect indicator for evaluating the degree of cell damage rate.

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“…Lavezzi et al used the number of AgNOR in the detection of neuronal damage in newborns. Eröz et al stated that the number of AgNOR in hair follicles increased with the effect of growth hormone. In addition to these reports, there is no study showing relationship between AgNOR and atopic dermatitis.…”

Section: Discussionmentioning

confidence: 99%

The relationship of argyrophilic proteins of the nuclear‐organized regions and atopic dermatitis in children

Gündüz

Okan

Şengil

et al. 2019

Experimental Dermatology

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Backround: The arginophilic proteins of nuclear organized regions (AgNOR), visualized with colloidal silver methods as black dots are known as AgNOR. To date, the relationship between AgNOR and cancer and inflammatory conditions has been investigated. However, there has been no report investigating the relationship between AgNOR and atopic dermatitis. The aim of this study was to investigate the relationship between atopic dermatitis and AgNOR in paediatric patients. Methods: Twenty-nine children with atopic dermatitis and 23 healthy children were included in the study. AgNOR test results were analysed prospectively. Results: The mean AgNOR number (40.19 ± 21.06) in the patient group was significantly higher than the control group (12.83 ± 10.40) (P < .001). Conclusions: This study investigated the association between AgNOR and atopic dermatitis for the first time in the literature. In the study, atopic dermatitis and AgNOR were found to be related. In the study, for the first time with the ROC analysis, AgNOR limit values with high sensitivity and specificity levels were determined in the diagnosis of atopic dermatitis.

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Argyrophilic nucleolar organizing region associated protein synthesis in hair root cells of humans at different developmental stages and sex

Cited by 22 publications

References 25 publications

Nuclear AgNOR protein enhancement in nucleoplasms of peripheral blood lymphocytes of babies/children with down syndrome

Nuclear AgNOR protein enhancement in nucleoplasms of peripheral blood lymphocytes of babies/children with down syndrome

Is one-time carbon monoxide intoxication harmless? Evaluation by argyrophilic nucleolar-organizing regions staining method

The relationship of argyrophilic proteins of the nuclear‐organized regions and atopic dermatitis in children

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