Argusides B and C, Two New Cytotoxic Triterpene Glycosides from the Sea Cucumber <i>Bohadschia argus</i><scp>Jaeger</scp>

Liu, Bao‐Shu; Yi, Yang‐Hua; Li, Ling; Sun, Peng; Yuan, Weiping; Sun, Guo-Quan; Han, Hua; Xue, Mei

doi:10.1002/cbdv.200890115

Cited by 31 publications

(10 citation statements)

References 6 publications

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“…Another study also showed that stichoposide C (isolated from the holothurian Thelenota anax) caused apoptosis in A549, HCT-116, and MCF-7 cells in a dose-dependent manner due to the activation of Fas and caspase-8, cleavage of Bid, mitochondrial damage, and caspase-3 activation ( 40 ).…”

Section: Discussionmentioning

confidence: 99%

Selective Toxicity of Persian Gulf Sea Cucumber (Holothuria parva) and Sponge (Haliclona oculata) Methanolic Extracts on Liver Mitochondria Isolated from an Animal Model of Hepatocellular Carcinoma

et al. 2015

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Background:Natural products isolated from marine environments are well known for their pharmacodynamic potential in diverse disease treatments, such as for cancer or inflammatory conditions. Sea cucumbers are marine animals of the phylum Echinoderm and the class Holothuroidea, with leathery skin and gelatinous bodies. Sponges are important components of Persian Gulf animal communities, and the marine sponges of the genus Haliclona have been known to display broad-spectrum biological activity. Many studies have shown that sea cucumbers and sponges contain antioxidants and anti-cancer compounds.Objectives:This study was designed to determine the selective toxicity of Persian Gulf sea cucumber (Holothuria parva) and sponge (Haliclona oculata) methanolic extracts on liver mitochondria isolated from an animal model of hepatocellular carcinoma, as part of a national project that hopes to identify novel potential anticancer candidates among Iranian Persian Gulf flora and fauna.Materials and Methods:To induce hepatocarcinogenesis, rats were given diethylnitrosamine (DEN) injections (200 mg/kg i.p. by a single dose), and then the cancer was promoted with 2-acetylaminofluorene (2-AAF) (0.02 w/w) for two weeks. Histopathological evaluations were performed, and levels of liver injury markers and a specific liver cancer marker (alpha-fetoprotein), were determined for confirmation of hepatocellular carcinoma induction. Finally, mitochondria were isolated from cancerous and non-cancerous hepatocytes.Results:Our results showed that H. parva methanolic extracts (250, 500, and 1000 µg/mL) and H. oculata methanolic extracts (200, 400, and 800 µg/mL) increased reactive oxygen species (ROS) formation, mitochondrial membrane potential (MMP), mitochondrial swelling, and cytochrome c release in the mitochondria obtained from cancerous hepatocytes, but not in mitochondria obtained from non-cancerous liver hepatocytes. These extracts also induced caspase-3 activation, which is known as a final mediator of apoptosis, in the hepatocytes obtained only from cancerous, not non-cancerous, rat livers.Conclusions:Our results suggest that H. parva and H. oculata may be promising therapeutic candidates for the treatment of HCC, following further confirmatory in vivo experiments and clinical trials.

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Section: Discussionmentioning

confidence: 99%

Selective Toxicity of Persian Gulf Sea Cucumber (Holothuria parva) and Sponge (Haliclona oculata) Methanolic Extracts on Liver Mitochondria Isolated from an Animal Model of Hepatocellular Carcinoma

et al. 2015

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“…For example, arguside A dramatically affects human colorectal carcinoma (HCT-116) cells (IC 50 = 0.14 μM) [ 61 ]. Argusides B and C also demonstrate potent efficacy against adenocarcinomic human alveolar basal epithelial cells (A549), HCT-116, Hep-G2, and human breast adenocarcinoma (MCF-7) cell lines with IC 50 values of 0.48 and 0.43 μg/mL, respectively, on A549 with 0.46 and 0.38 μg/mL, respectively, on HCT-116 [ 62 ]. Argusides D and E were shown to be very effective regarding all aforementioned human tumor cell lines [ 63 ].…”

Section: Biologically Active Compounds With Anticancer Activitymentioning

confidence: 99%

Pharmacological Potential of Sea Cucumbers

Khotimchenko

2018

IJMS

129

103

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This review presents a detailed analysis of published research data focused on the pharmacological activity exerted by biologically active compounds isolated from sea cucumbers belonging to the class of Holothuroidea, phylum Echinodermata. The review contains descriptions of the structure, physico-chemical properties and pharmacological effects of these active substances. Particular attention is given to compounds with anticoagulant, antithrombotic, antioxidant, anticancer, anti-infectious, immune-stimulating and anti-ACE (angiotensin converting enzyme) activities as well as to the substances exerting a regulating influence on lipid and carbohydrate metabolism. All these compounds may be considered as prototypes for development of new pharmaceutical substances and medicines.

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“…They are reported primarily as typical metabolites of terrestrial plants [25]. A few marine species belonging to the phylum Echinodermata [26] namely holothuroids (sea cucumbers) [7,10,13,27,28,29,30,31,32,33] and asteroids, and sponges from the phylum Porifera [13,34,35] produce saponins.…”

Section: Introductionmentioning

confidence: 99%

Discovery of Novel Saponins from the Viscera of the Sea Cucumber Holothuria lessoni

2014

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Sea cucumbers, sometimes referred to as marine ginseng, produce numerous compounds with diverse functions and are potential sources of active ingredients for agricultural, nutraceutical, pharmaceutical and cosmeceutical products. We examined the viscera of an Australian sea cucumber Holothuria lessoni Massin et al. 2009, for novel bioactive compounds, with an emphasis on the triterpene glycosides, saponins. The viscera were extracted with 70% ethanol, and this extract was purified by a liquid-liquid partition process and column chromatography, followed by isobutanol extraction. The isobutanol saponin-enriched mixture was further purified by high performance centrifugal partition chromatography (HPCPC) with high purity and recovery. The resultant purified polar samples were analyzed using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)/MS and electrospray ionization mass spectrometry (ESI-MS)/MS to identify saponins and characterize their molecular structures. As a result, at least 39 new saponins were identified in the viscera of H. lessoni with a high structural diversity, and another 36 reported triterpene glycosides, containing different aglycones and sugar moieties. Viscera samples have provided a higher diversity and yield of compounds than observed from the body wall. The high structural diversity and novelty of saponins from H. lessoni with potential functional activities presents a great opportunity to exploit their applications for industrial, agricultural and pharmaceutical use.

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Argusides B and C, Two New Cytotoxic Triterpene Glycosides from the Sea Cucumber Bohadschia argusJaeger

Cited by 31 publications

References 6 publications

Selective Toxicity of Persian Gulf Sea Cucumber (Holothuria parva) and Sponge (Haliclona oculata) Methanolic Extracts on Liver Mitochondria Isolated from an Animal Model of Hepatocellular Carcinoma

Selective Toxicity of Persian Gulf Sea Cucumber (Holothuria parva) and Sponge (Haliclona oculata) Methanolic Extracts on Liver Mitochondria Isolated from an Animal Model of Hepatocellular Carcinoma

Pharmacological Potential of Sea Cucumbers

Discovery of Novel Saponins from the Viscera of the Sea Cucumber Holothuria lessoni

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