Argonaute4 Modulates Resistance to <i>Fusarium</i><i>brachygibbosum</i> Infection by Regulating Jasmonic Acid Signaling

Pradhan, Maitree; Pandey, Priyanka; Baldwin, Ian Thomas; Pandey, Sarvesh

doi:10.1104/pp.20.00171

Cited by 34 publications

(38 citation statements)

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“…Besides, jasmonate-deficient tomato mutants showed hypersusceptibility to the pathogen [ 31 ], and exogenous JA treatments in wheat reduced both the symptoms of fusarium wilt and mycelia growth [ 32 ]. Most recent studies suggest that host– Fusarium interaction is governed by JA, and that compromised JA levels are associated with increased susceptibility [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Signaling in the Tomato Immunity against Fusarium oxysporum

et al. 2021

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New strategies of control need to be developed with the aim of economic and environmental sustainability in plant and crop protection. Metabolomics is an excellent platform for both understanding the complex plant–pathogen interactions and unraveling new chemical control strategies. GC-MS-based metabolomics, along with a phytohormone analysis of a compatible and incompatible interaction between tomato plants and Fusarium oxysporum f. sp. lycopersici, revealed the specific volatile chemical composition and the plant signals associated with them. The susceptible tomato plants were characterized by the over-emission of methyl- and ethyl-salicylate as well as some fatty acid derivatives, along with an activation of salicylic acid and abscisic acid signaling. In contrast, terpenoids, benzenoids, and 2-ethylhexanoic acid were differentially emitted by plants undergoing an incompatible interaction, together with the activation of the jasmonic acid (JA) pathway. In accordance with this response, a higher expression of several genes participating in the biosynthesis of these volatiles, such as MTS1, TomloxC,TomloxD, and AOS, as well as JAZ7, a JA marker gene, was found to be induced by the fungus in these resistant plants. The characterized metabolome of the immune tomato plants could lead to the development of new resistance inducers against Fusarium wilt treatment.

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Section: Discussionmentioning

confidence: 99%

Signaling in the Tomato Immunity against Fusarium oxysporum

et al. 2021

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“…To evaluate if Na‐miR390 is involved in the resistance response to a natural fungal pathogen, single leaf inoculations with Fusarium brachygibbosum Utah 4 isolates were performed (Pradhan et al, 2020 ), and the development of visible disease symptoms was recorded. No significant differences in the diameter of chlorotic/necrotic lesions of WT and miR390‐overexpressing plants were observed during infection with the Fusarium pathogen (Figures 2b and S3 ).…”

Section: Resultsmentioning

confidence: 99%

“…miRNAs are thought to play indispensable roles in a plant's phenotypic plasticity, being involved in the regulation of differentiation, development and reproduction, and mediating responses to environmental fluctuations (Brant & Budak, 2018 ; Li et al, 2017 ; Song et al, 2019 ). In studies that have manipulated the expression of different components of the small‐RNA (smRNA) pathways, evidence for roles in the diverse plastic responses that allow plants to adapt to environmental challenges in natural habitats has been reported (Pandey et al, 2008 ; Pandey & Baldwin, 2007 ; Pradhan et al, 2017 , 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…The effectors of miRNA action, the Argonaute (AGO) proteins, are conserved across all lineages of photosynthetic organisms (Pradhan et al, 2020 ), indicating that miRNA‐driven regulation of gene expression could be ancient. However, individual miRNAs/miRNA‐families appear to evolve rapidly, as suggested by their frequent birth and loss in lineage‐ and species‐specific manners (Cuperus et al, 2011 ; Nozawa et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, the composition and role of the RNA‐directed RNA polymerase (RdR), DCL, and AGO components of the smRNA machinery during adaptation to these natural challenges have also been studied (Navarro‐Quezada et al, 2020 ). The composition of miRNAs in N. attenuata's genome (Xu et al, 2017 ) and its reprograming during herbivore attack, pathogen infection, and AMF colonization are also known (Pandey et al, 2018 ; Pradhan et al, 2017 , 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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microRNA390 modulates Nicotiana attenuata's tolerance response to Manduca sexta herbivory

et al. 2021

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miR390 is a highly conserved miRNA in plant lineages known to function in growth and development processes, such as lateral root development, and in responses to salt and metal stress. In the ecological model species, Nicotiana attenuata , miR390's biological function remains unknown, which we explore here with a gain‐of‐function analysis with plants over‐expressing (OE‐) N. attenuata miR390 (Na‐miR390) in glasshouse and natural environments. OEmiR390 plants showed normal developmental processes, including lateral root formation or reproductive output, in plants grown under standard conditions in the glasshouse. OEmiR390 plants did not have dramatically altered interactions with arbuscular mycorrhizal fungi (AMF), Fusarium pathogens, or herbivores. However, Na‐miR390 regulated the plant's tolerance of herbivory. Caterpillar feeding elicits the accumulation of a suite of phytohormones, including auxin and jasmonates, which further regulate host‐tolerance. The increase in Na‐miR390 abundance reduces the accumulation of auxin but does not influence levels of other phytohormones including jasmonates (JA, JA‐Ile), salicylic acid (SA), and abscisic acid (ABA). Na‐miR390 overexpression reduces reproductive output, quantified as capsule production, when plants are attacked by herbivores. Exogenous auxin treatments of herbivore‐attacked plants restored capsule production to wild‐type levels. During herbivory, Na‐miR390 transcript abundances are increased; its overexpression reduces the abundances of auxin biosynthesizing YUCCA and ARF (mainly ARF4 ) transcripts during herbivory. Furthermore, the accumulation of auxin‐regulated phenolamide secondary metabolites (caffeoylputrescine, dicaffeoylspermidine) is also reduced. In N. attenuata , miR390 functions in modulating tolerance responses of herbivore‐attacked plants.

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Barley AGO4 proteins show overlapping functionality with distinct small RNA-binding properties in heterologous complementation

Miloro,

Kis,

Havelda

et al. 2024

Plant Cell Rep

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Key message Barley AGO4 proteins complement expressional changes of epigenetically regulated genes in Arabidopsis ago4-3 mutant and show a distinct affinity for the 5′ terminal nucleotide of small RNAs, demonstrating functional conservation and divergence. Abstract The function of Argonaute 4 (AGO4) in Arabidopsis thaliana has been extensively characterized; however, its role in monocots, which have large genomes abundantly supplemented with transposable elements (TEs), remains elusive. The study of barley AGO4 proteins can provide insights into the conserved aspects of RNA-directed DNA methylation (RdDM) and could also have further applications in the field of epigenetics or crop improvement. Bioinformatic analysis of RNA sequencing data identified two active AGO4 genes in barley, HvAGO4a and HvAGO4b. These genes function similar to AtAGO4 in an Arabidopsis heterologous complementation system, primarily binding to 24-nucleotide long small RNAs (sRNAs) and triggering methylation at specific target loci. Like AtAGO4, HvAGO4B exhibits a preference for binding sRNAs with 5′ adenine residue, while also accepting 5′ guanine, uracil, and cytosine residues. In contrast, HvAGO4A selectively binds only sRNAs with a 5′ adenine residue. The diverse binding capacity of barley AGO4 proteins is reflected in TE-derived sRNAs and in their varying abundance. Both barley AGO4 proteins effectively restore the levels of extrachromosomal DNA and transcript abundancy of the heat-activated ONSEN retrotransposon to those observed in wild-type Arabidopsis plants. Our study provides insight into the distinct binding specificities and involvement in TE regulation of barley AGO4 proteins in Arabidopsis by heterologous complementation.

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Argonaute4 Modulates Resistance to Fusariumbrachygibbosum Infection by Regulating Jasmonic Acid Signaling

Cited by 34 publications

References 100 publications

Signaling in the Tomato Immunity against Fusarium oxysporum

Signaling in the Tomato Immunity against Fusarium oxysporum

microRNA390 modulates Nicotiana attenuata's tolerance response to Manduca sexta herbivory

Barley AGO4 proteins show overlapping functionality with distinct small RNA-binding properties in heterologous complementation

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