Argon Laser Photocoagulation–Induced Modification of Gene Expression in the Retina

Wilson, Ann; Hobbs, Bridget G.; Shen, Weiyong; Speed, T. P.; Schmidt, Uli; Begley, C. Glenn; Rakoczy, P. Elizabeth

doi:10.1167/iovs.02-0622

Cited by 73 publications

(57 citation statements)

References 34 publications

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“…We believe that the effectiveness of SDM PRP we have observed at the low laser irradiance levels employed in this and our previous report of SDM for diabetic macular oedema support the relevance of SDM theory in clinical practice. 19,40,41 This study reviews the experience of a single surgeon employing a novel photocoagulation strategy in a clinical setting to treat diabetic retinopathy. Although visual acuity outcomes were assessed, other tests of retinal function such as visual field testing and electrophysiology were not performed.…”

Section: Discussionmentioning

confidence: 99%

Subthreshold diode micropulse panretinal photocoagulation for proliferative diabetic retinopathy

Luttrull

Musch

Spink

2007

Eye

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Section: Discussionmentioning

confidence: 99%

Subthreshold diode micropulse panretinal photocoagulation for proliferative diabetic retinopathy

Luttrull

Musch

Spink

2007

Eye

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“…Laser burns destroy the ischemic retinal tissue that is a source of intraocular VEGF (25,26), which is followed by alterations in gene expression, as has been observed in mice retina after PRP. Specifically, genes inhibiting VEGF expression (e.g., angiotensin II type 2 receptor) were found to be upregulated, whereas genes promoting VEGF expression (e.g., fibroblast growth factors 14 and 16, interleukin-1␤, calcitonin receptor-like receptor, and plasminogen activator inhibitor 2) were found to be downregulated (27). These changes may lower the intraocular VEGF levels and hence favor the initial therapeutic response to PRP.…”

Section: Research Design Andmentioning

confidence: 96%

Metabolic Control of Diabetes Is Associated With an Improved Response of Diabetic Retinopathy to Panretinal Photocoagulation

Kotoula

Koukoulis²,

Ζιντζαράς³

et al. 2005

Diabetes Care

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OBJECTIVE -To study the influence of glycemic control and the presence of microalbuminuria on the initial response to panretinal photocoagulation (PRP) in patients with a high-risk proliferative diabetic retinopathy (PDR).RESEARCH DESIGN AND METHODS -This was a prospective cohort study with a two-by-two factorial design. We used full-scattered PRP to treat 115 eyes of type 2 diabetic patients who have high-risk PDR. HbA 1c (A1C) and albumin levels in 24-h urine were constantly monitored during the preenrollment, treatment, and posttreatment periods. At a follow-up visit 12 weeks after the last PRP session, the fundus was examined for characteristics of regression from high-risk PDR and the response to PRP was determined to be successful or unsuccessful. The eyes were categorized into four groups based on average A1C levels and the presence or absence of microalbuminuria. The data were analyzed using a logistic regression model. Our statistical analysis determined the probability of achieving a satisfactory response to PRP in association with A1C levels and the presence or absence of microalbuminuria.RESULTS -Of the 115 eyes examined, 65 (56.5%) had a successful initial response to PRP and 50 (43.5%) did not. The probability of a satisfactory response to PRP was related to A1C levels (P Ͻ 0.05) but not to microalbuminuria and its interaction with hemoglobin glycosylation (P Ն 0.05).CONCLUSIONS -Low levels of hemoglobin glycosylation (A1C Ͻ8%) during the pretreatment, treatment, and posttreatment periods are associated with a regression of proliferative diabetic retinopathy after PRP. Diabetes Care 28:2454 -2457, 2005D iabetic retinopathy has been the subject of in-depth study for the last 3 decades, during which time randomized and controlled multicenter clinical trials have provided important information about the natural history and treatment of the disease. Panretinal photocoagulation (PRP) is the treatment of choice for proliferative diabetic retinopathy (PDR) (1). However, some patients do not respond to PRP and eventually experience legal blindness (2).Hyperglycemia is an important risk factor for the development of microvascular disease in diabetic patients. There is a direct relation between the degree of glycemic control and the incidence and progression of retinopathy (3-11). However, the influence of metabolic control of diabetes on the response of diabetic retinopathy to PRP has not been previously studied. Linking PRP effectiveness with metabolic control of diabetes may provide useful information for visual prognosis in diabetic patients.Data from most studies suggest an association between diabetic nephropathy, as manifested by microalbuminuria, and diabetic retinopathy (12). There are no data, however, on whether the presence of microalbuminuria is a negative prognostic factor for the efficacy of PRP. The aim of this study was to assess the influence of the degree of glycemic control and microalbuminuria on the initial response to PRP in patients with high-risk PDR. RESEARCH DESIGN AND METHODS -Thi...

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“…Apart from taking into account the main confounding factors, discussed above (vitreous haemorrhage and intravitreal protein concentration) subjects who had undergone laser photocoagulation in the preceding 6 months were excluded. This is because a significant alteration in the balance of intravitreal growth factors and transcriptional activity in the retina has been shown following this procedure [12]. To carry out this comparative proteomic analysis, we used fluorescence-based difference gel electrophoresis (DIGE) [13].…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of human vitreous fluid by fluorescence-based difference gel electrophoresis (DIGE): a new strategy for identifying potential candidates in the pathogenesis of proliferative diabetic retinopathy

Garcia-Ramírez

Canals

Hernández³

et al. 2007

Diabetologia

151

135

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Aims/hypothesis The aim of this study was to compare the protein profile of vitreous fluid from diabetic patients with proliferative diabetic retinopathy (PDR) with that from nondiabetic patients with idiopathic macular holes (MH). The mRNA of proteins differentially produced was also assessed in the retinas from diabetic and non-diabetic donors. Materials and methods Vitreous humour from type 1 diabetic patients with PDR (n=8) and from non-diabetic patients with MH (n=10) closely matched in terms of age were studied. The comparative proteomic analysis was performed using fluorescence-based difference gel electrophoresis (DIGE). Differentially produced proteins (abundance ratio >1.4, p<0.05) were identified by mass spectrometry. Expressions of mRNA were measured by real-time RT-PCR in retinas from ten human eyes obtained at post-mortem (five eyes from diabetic subjects and five eyes from non-diabetic subjects). Results Eight proteins were highly produced in PDR patients in comparison with non-diabetic subjects: zinc-α 2 -glycoprotein (ZAG), apolipoprotein (apo) A1, apoH, fibrinogen A, and the complement factors C3, C4b, C9 and factor B). We found three proteins that were underproduced in PDR subjects: pigment epithelial derived factor (PEDF), interstitial retinol-binding protein (IRBP) and inter-α-trypsin inhibitor heavy chain (ITIH2). There was no overlap in the vitreous levels of the above-mentioned proteins between PDR patients and non-diabetic control subjects. The differential production of ZAG, C3, factor B, PEDF and IRBP was further confirmed by western blot, and was in agreement with mRNA levels detected in the retina. Conclusions/interpretation Proteomic analysis by DIGE, which permits an accurate quantitative comparison, was useful in identifying new potential candidates involved in the pathogenesis of PDR.

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Argon Laser Photocoagulation–Induced Modification of Gene Expression in the Retina

Cited by 73 publications

References 34 publications

Subthreshold diode micropulse panretinal photocoagulation for proliferative diabetic retinopathy

Subthreshold diode micropulse panretinal photocoagulation for proliferative diabetic retinopathy

Metabolic Control of Diabetes Is Associated With an Improved Response of Diabetic Retinopathy to Panretinal Photocoagulation

Proteomic analysis of human vitreous fluid by fluorescence-based difference gel electrophoresis (DIGE): a new strategy for identifying potential candidates in the pathogenesis of proliferative diabetic retinopathy

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