Arginine vasotocin (AVT)/mesotocin (MT) receptors in chickens: Evidence for the possible involvement of AVT-AVPR1 signaling in the regulation of oviposition and pituitary prolactin expression

Wu, Chao; Lv, Can; Wan, Ying; Li, Xiaoxiao; Zhang, Jiannan; Li, Juan; Wang, Yajun

doi:10.1016/j.ygcen.2019.05.013

Cited by 22 publications

(23 citation statements)

References 74 publications

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“…In this study, we found that many receptors are expressed in endocrine/non-endocrine cell clusters (Figure 6). All these findings not only support previous pioneering findings regarding the hormonal regulation of avian pituitary gonadotrophs (e.g., GnRH and gonadal steroids) (Hall et al, 1986;Sharp et al, 1987;Millar, 2005), lactotrophs (VIP, dopamine, CORT, and NPW) (Macnamee et al, 1986;el Halawani et al, 1990;Al Kahtane et al, 2003;Fu and Porter, 2004;Bu et al, 2016;Scanes, 2016;Lv et al, 2018), thyrotroph (CRH, T3, GCGL and SST) (De Groef et al, 2003;De Groef et al, 2007;Huang et al, 2014), and corticotroph functions (AVT, CRH, and CORT) (Mikhailova et al, 2007;Selvam et al, 2013;Wu et al, 2019), but also hints that many novel signals from the hypothalamus and peripheral tissues (e.g., NPW, serotonin, SST, insulin, PP, and PRLH) could regulate pituitary cell functions, in which nearly all functional axes (e.g., HPG, HPT, and HPA) are inter-connected. Meanwhile, our findings also strongly suggest that peripheral signals from the adipose tissue (adiponectin), gastrointestinal tract (serotonin), and pancreas (GCG, INS, SST, and PP) are likely involved in the regulation of avian pituitary functions.…”

Section: Receptors Expressed In Pituitary Cell Clusterssupporting

confidence: 85%

Single-Cell RNA Sequencing Analysis of Chicken Anterior Pituitary: A Bird’s-Eye View on Vertebrate Pituitary

Zhang

et al. 2021

Front. Physiol.

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It is well-established that anterior pituitary contains multiple endocrine cell populations, and each of them can secrete one/two hormone(s) to regulate vital physiological processes of vertebrates. However, the gene expression profiles of each pituitary cell population remains poorly characterized in most vertebrate groups. Here we analyzed the transcriptome of each cell population in adult chicken anterior pituitaries using single-cell RNA sequencing technology. The results showed that: (1) four out of five known endocrine cell clusters have been identified and designated as the lactotrophs, thyrotrophs, corticotrophs, and gonadotrophs, respectively. Somatotrophs were not analyzed in the current study. Each cell cluster can express at least one known endocrine hormone, and novel marker genes (e.g., CD24 and HSPB1 in lactotrophs, NPBWR2 and NDRG1 in corticotrophs; DIO2 and SOUL in thyrotrophs, C5H11ORF96 and HPGDS in gonadotrophs) are identified. Interestingly, gonadotrophs were shown to abundantly express five peptide hormones: FSH, LH, GRP, CART and RLN3; (2) four non-endocrine/secretory cell types, including endothelial cells (expressing IGFBP7 and CFD) and folliculo-stellate cells (FS-cells, expressing S100A6 and S100A10), were identified in chicken anterior pituitaries. Among them, FS-cells can express many growth factors, peptides (e.g., WNT5A, HBEGF, Activins, VEGFC, NPY, and BMP4), and progenitor/stem cell-associated genes (e.g., Notch signaling components, CDH1), implying that the FS-cell cluster may act as a paracrine/autocrine signaling center and enrich pituitary progenitor/stem cells; (3) sexually dimorphic expression of many genes were identified in most cell clusters, including gonadotrophs and lactotrophs. Taken together, our data provides a bird’s-eye view on the diverse aspects of anterior pituitaries, including cell composition, heterogeneity, cell-to-cell communication, and gene expression profiles, which facilitates our comprehensive understanding of vertebrate pituitary biology.

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Section: Receptors Expressed In Pituitary Cell Clusterssupporting

confidence: 85%

Single-Cell RNA Sequencing Analysis of Chicken Anterior Pituitary: A Bird’s-Eye View on Vertebrate Pituitary

Zhang

et al. 2021

Front. Physiol.

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“…Furthermore, almost all DEGs enriched in this pathway, including OPRM1, F2RL2, GLRA2, HTR7, HTR2B, OPRK1, P2RY13, GRM4, LEPR, HTR1B, LPAR3, AVPR1A, and GABRP, were significantly upregulated in the ovarian stroma of SWG. As a receptor for oxytocin, AVPR1A was shown to mediate the secretion of ACTH and PRL by binding AVT to finely regulate ovulation in chickens [31]. Expression levels of OPRK1 were closely related to oocyte maturation [32] and maternal behavior [33].…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis Suggests Key Roles for 5-Hydroxytryptamlne Receptors in Control of Goose Egg Production

Ouyang

Wang

et al. 2020

Genes

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To date, research on poultry egg production performance has only been conducted within inter or intra-breed groups, while those combining both inter- and intra-breed groups are lacking. Egg production performance is known to differ markedly between Sichuan white goose (Anser cygnoides) and Landes goose (Anser anser). In order to understand the mechanism of egg production performance in geese, we undertook this study. Here, 18 ovarian stromal samples from both Sichuan white goose and Landes goose at the age of 145 days (3 individuals before egg production initiation for each breed) and 730 days (3 high- and low egg production individuals during non-laying periods for each breed) were collected to reveal the genome-wide expression profiles of ovarian mRNAs and lncRNAs between these two geese breeds at different physiological stages. Briefly, 58, 347, 797, 777, and 881 differentially expressed genes (DEGs) and 56, 24, 154, 105, and 224 differentially expressed long non-coding RNAs (DElncRNAs) were found in LLD vs. HLD (low egg production Landes goose vs. high egg production Landes goose), LSC vs. HSC (low egg production Sichuan White goose vs. high egg production Sichuan white goose), YLD vs. YSC (young Landes goose vs. young Sichuan white goose), HLD vs. HSC (high egg production Landes goose vs. high egg production Sichuan white goose), and LLD vs. LSC (low egg production Landes goose vs. low egg production Sichuan white goose) groups, respectively. Functional enrichment analysis of these DEGs and DElncRNAs suggest that the “neuroactive ligand–receptor interaction pathway” is crucial for egg production, and particularly, members of the 5-hydroxytryptamine receptor (HTR) family affect egg production by regulating ovarian metabolic function. Furthermore, the big differences in the secondary structures among HTR1F and HTR1B, HTR2B, and HTR7 may lead to their different expression patterns in goose ovaries of both inter- and intra-breed groups. These results provide novel insights into the mechanisms regulating poultry egg production performance.

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“…Total RNA was isolated by RNAzol (MRC, Cincinnati, OH) according to the manufacturer's instructions and dissolved in diethylpyrocarbonate-treated H 2 O (DEPC-H 2 O). Total RNA was reverse transcribed into cDNA using Moloney murine leukemia virus (MMLV) reverse transcriptase (Takara, Dalian, China), as described in our recent study (32). In brief, oligodeoxythymide and total RNA (2 μg) were mixed in a total volume of 5 μL, incubated at 70 °C for 10 min, and cooled at 4 °C for 2 min.…”

Section: Methodsmentioning

confidence: 99%

“…Reverse transcription (RT) was performed at 42 °C for 90 min. RT negative controls were performed under the same condition without the addition of reverse transcriptase (32).…”

Section: Methodsmentioning

confidence: 99%

Identification of a Novel Functional Corticotropin-Releasing Hormone (CRH2) in Chickens and Its Roles in Stimulating Pituitary TSHβ Expression and ACTH Secretion

Fan

Yang

et al. 2019

Front. Endocrinol.

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Corticotropin-releasing hormone (CRH), together with its structurally and functionally related neuropeptides, constitute the CRH family and play critical roles in multiple physiological processes. Recently, a novel member of this family, namely CRH2, was identified in vertebrates, however, its functionality and physiological roles remain an open question. In this study, using chicken (c-) as the animal model, we characterized the expression and functionality of CRH2 and investigated its roles in anterior pituitary. Our results showed that (1) cCRH2 cDNA is predicted to encode a 40-aa mature peptide, which shares a higher amino acid sequence identity to cCRH (63%) than to other CRH family peptides (23–38%); (2) Using pGL3-CRE-luciferase reporter system, we demonstrated that cCRH2 is ~15 fold more potent in activating cCRH receptor 2 (CRHR2) than cCRHR1 when expressed in CHO cells, indicating that cCRH2 is bioactive and its action is mainly mediated by CRHR2; (3) Quantitative real-time PCR revealed that c CRH2 is widely expressed in chicken tissues including the hypothalamus and anterior pituitary, and its transcription is likely controlled by promoters near exon 1, which display strong promoter activity in cultured DF-1 and HEK293 cells; (4) In cultured chick pituitary cells, cCRH2 potently stimulates TSH β expression and shows a lower potency in inducing ACTH secretion, indicating that pituitary/hypothalamic CRH2 can regulate pituitary functions. Collectively, our data provides the first piece of evidence to suggest that CRH2 play roles similar, but non-identical, to those of CRH, such as its differential actions on pituitary, and this helps to elucidate the roles of CRH2 in vertebrates.

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Arginine vasotocin (AVT)/mesotocin (MT) receptors in chickens: Evidence for the possible involvement of AVT-AVPR1 signaling in the regulation of oviposition and pituitary prolactin expression

Cited by 22 publications

References 74 publications

Single-Cell RNA Sequencing Analysis of Chicken Anterior Pituitary: A Bird’s-Eye View on Vertebrate Pituitary

Single-Cell RNA Sequencing Analysis of Chicken Anterior Pituitary: A Bird’s-Eye View on Vertebrate Pituitary

Comparative Transcriptome Analysis Suggests Key Roles for 5-Hydroxytryptamlne Receptors in Control of Goose Egg Production

Identification of a Novel Functional Corticotropin-Releasing Hormone (CRH2) in Chickens and Its Roles in Stimulating Pituitary TSHβ Expression and ACTH Secretion

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