Arginine uptake by rabbit spermatozoa

Radany, E. W.; Atherton, R. W.; Forrester, Ian T.

doi:10.1016/0003-9861(81)90244-7

Cited by 11 publications

(9 citation statements)

References 24 publications

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“…The above observation is in agreement with the report from Radany et al (1981), in which competitive experiments with L-arginine analogs and amino acids show the active role of the guanidino group in L-arginine transport across the sperm cell. This indicates that L-arginine plays an important role in enhancing membrane permeability and thereby increased substrate permeation/utilization and concomitant lactate production.…”

Section: Mode Of Action Of Argininesupporting

confidence: 93%

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Arginine Activates Glycolysis of Goat Epididymal Spermatozoa: An NMR Study

Patel

Srivastava

Phadke

et al. 1998

Biophysical Journal

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The present study explores the mechanism underlying the action of L-arginine on the metabolic activity of spermatozoa. Goat epididymal spermatozoa were incubated with different concentrations of L-arginine to determine its effect on the utilization of glucose, fructose, and pyruvate. NMR techniques have been applied to elucidate the effect of L-arginine, L-lysine, and L-ornithine on the glycolysis of epididymal goat spermatozoa. Whereas 31P NMR has been used to estimate the change of pH in the presence of different concentrations of L-arginine, 13C NMR has been used to estimate the substrate consumption and lactate production. At optimal concentration of L-arginine, the forward metabolic rates have been found to increase by two to three times over control experiments. Arginine is not consumed in these reactions, but acts as an activator. Longitudinal relaxation time (T1) measurements indicate that the guanidino group of L-arginine plays an active role in binding to cells. The amino acid L-lysine is less effective, and L-ornithine is ineffective.

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Section: Mode Of Action Of Argininesupporting

confidence: 93%

“…These molecules enter the glycolytic pathway at different stages. The requisite amount of L-arginine was added to the cell suspension, and incubation was allowed for half an hour for equilibration (Radany et al, 1981). The substrates were then added, and 13 C NMR spectra were recorded as a function of time.…”

Section: Cellular Metabolismmentioning

confidence: 99%

Arginine Activates Glycolysis of Goat Epididymal Spermatozoa: An NMR Study

Patel

Srivastava

Phadke

et al. 1998

Biophysical Journal

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“…L-arginine is amino acid plays an important role in stimulating sperm motility in rabbits (Radany et al, 1981), humans (Aydin et al, 1995) and goats (Patel et al, 1998) under in vitro conditions. L-arginine prevents membrane lipid peroxidation in spermatozoa under different peroxidation conditions (Srivastava et al, 2006, Evan et.…”

Section: Introductionmentioning

confidence: 99%

Effect of Addition L-Arginine to Extender of Poor Motile Holstein Bulls Spermatozoa.

Abdallah

Eliraqy

2019

Journal of Productivity and Development

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This study was carried out to investigate the effect of addition of different levels of L-arginine (0, 3, 5, 7 and 10 mM) to improve the poor motile of Holstein bulls spermatozoa. Semen collected from five Holstein bulls once weekly and ejaculates with 45-50% mass motility was pooled. Semen was diluted with Tris-egg yolk extender at 37C° and divided into five parts,1 st part without supplement (control), other four parts 2 nd , 3 rd , 4 th and 5 th were supplemented with 3, 5, 7 and 10 mM Larginine respectively. Diluted semen was equilibrated at 4 C° for 4 h, then, filled in 0.25 ml French straws. Straws were kept on liquid nitrogen vapors for 10 min and then plunged and stored in liquid nitrogen at -196C°. Progressive sperm motility, live sperm, abnormal sperm and sperm acrosome integrity were determined post-dilution, equilibration and post-thawing processing.The results were referred that extenders with concentrations of 5 and 7mM L-Arginine were improved all parameters studied than 3; 10 mM L-arginine extenders and control extender at different cryopreservation processes. The percentage of DNA damage were 20. 0, 10.8, 4.40, 3.20 and 9.40 for control and 3, 5, 7 and 10 mM L-arginine extenders respectively. Significant differences in chromatin integrity were observed among control and treatments (P<0. 05). Also, fertility rate was higher in Friesian cows artificially inseminated with 5 and 7 mM L-arginine (65 and 66%) compared with control (45%).Conclusively, it could be concluded that adding L-arginine with concentrations of 5 or 7mM to Tris-extender improved the freezability and fertility of poor quality of Holstein bull spermatozoa.

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“…The effect of l‐ arginine on sperm motility was tested using the Sander‐Cramer test (Sander & Cramer, 1941). Semen samples were previously incubated with Tyrode medium (control) or with the required quantity of l‐ arginine solution for 30 min, which is the optimum time for maximum uptake and equilibration by the cells (Randany et al. , 1981).…”

Section: Methodsmentioning

confidence: 99%

Effect of anion channel blockers on l-arginine action in spermatozoa from asthenospermic men

Srivastava

Agarwal

2010

Andrologia

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In earlier studies, we have established that l-arginine enhances motility and metabolic rate in spermatozoa of goat, bull and mouse. In the present study this work was extended to human sperm cells obtained from the semen samples of asthenospermic patients, which are characterised by low motility. The metabolic rate was followed by monitoring the glucose consumption (1-(13)C glucose as substrate) and the production of lactate in sperm cells, using (13)C NMR. The stimulatory effect of l-arginine was neutralised on adding an NO-synthase inhibitor like N(omega)-nitro-L-arginine methyl ester. On the other hand, the inactive d-enantiomorph did not affect the stimulatory effect of l-arginine. This strongly suggests that L-arginine acts through the NO signal pathway. We also demonstrated that the stimulatory effect of L-arginine was inhibited in the presence of anion channel inhibitors like 4-acetamido-4'-isothiocyanostilbene-2,2'-disulphonic acid, 2,4-dinitrophenol and carbonyl cyanide m-chlorophenylhydrazone. Furthermore, bicarbonate supplementation was found to be essential for the action of L-arginine. These observations indicate that L-arginine induces NO synthesis and stimulates motility and metabolism only when an active anion transport system is present.

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Arginine uptake by rabbit spermatozoa

Cited by 11 publications

References 24 publications

Arginine Activates Glycolysis of Goat Epididymal Spermatozoa: An NMR Study

Arginine Activates Glycolysis of Goat Epididymal Spermatozoa: An NMR Study

Effect of Addition L-Arginine to Extender of Poor Motile Holstein Bulls Spermatozoa.

Effect of anion channel blockers on l-arginine action in spermatozoa from asthenospermic men

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